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Investigation of the internal bacterial flora of Eurygaster integriceps (Hemiptera: Scutelleridae) and pathogenicity of the flora members

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Abstract

Eurygaster integriceps (Hemiptera: Scutelleridae) is one of the most serious pest of wheat and other cereal crops throughout the Near and Middle East including Turkey. To contribute biological control of this pest, we investigated the culturable bacterial flora of E. integriceps and their virulence against it. We also tested different entomopathogenic fungi (Isaria fumosorosea ARSEF8356, Beauveria bassiana ARSEF8356, Metarhizium brunneum ARSEF8671 and Nomurae rileyi ARSEF1670) against this pest under laboratory conditions. Bacterial isolates were characterized based on morphological, biochemical, physiological and molecular characteristics (16S rRNA sequencing). The isolates were identified as Pantoea sp. (S1, S5, S7, S8, S10 and S11), P. agglomerans (S2, S3 and S4), Pseudomonas sp. S6 and Micrococcus luteus S9. The highest mortality within bacterial isolates was observed from Pantoea sp. S1, P. agglomerans S4 and Pantoea sp. S7 with 100%. Mortality of other bacterial species ranged from 33% to 88%. The highest mortality among the tested entomopathogenic fungi was obtained from Isaria fumosorosea ARSEF 8333 with 100%. Mortality of other fungi ranged from 33% to 50%. Consequently, Pantoea sp. S1, P. agglomerans S4, Pantoea sp. S7, Beauveria bassiana ARSEF 8356 and Isaria fumosorosea ARSEF 8356 seem to be promising candidates in the control of E. integriceps.

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Abbreviations

API:

Analytical profile index

ARSEF:

ARS Collection of Entomopathogenic Fungal Cultures

cfu:

Colonyforming unit

KIA:

Kligler Iron Agar

LSD:

Least Significant Difference

MEGA:

Molecular Evolutionary Genetics Analysis

NCCL:

National Committee for Clinical Laboratory

NJ:

Neighbor joining

SPSS:

Statistical Package for the Social Sciences

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Authors and Affiliations

  1. Department of Biology, Faculty of Arts and Sciences, Recep Tayyip Erdoğan University, 53100, Rize, Turkey

    Ömer Çelebi

  2. Faculty of Engineering and Architecture, Genetic and Bioengineering, Ahi Evran University, Kırşehir, 40100, Turkey

    Elif Sevim & Ali Sevim

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  1. Ömer Çelebi
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  2. Elif Sevim
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  3. Ali Sevim
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Correspondence to Ali Sevim.

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Çelebi, Ö., Sevim, E. & Sevim, A. Investigation of the internal bacterial flora of Eurygaster integriceps (Hemiptera: Scutelleridae) and pathogenicity of the flora members. Biologia 69, 1365–1375 (2014). https://doi.org/10.2478/s11756-014-0445-x

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