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Pollen morphology and anatomical features of Lilium (Liliaceae) taxa from Turkey

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Abstract

In this study, pollen grains and anatomical features of Turkish lilies were investigated under the electron (SEM) and light (LM) microscope. LM and SEM observations showed that the pollen grains are monosulcate, heteropolar, elliptical in polar view and oblate. Numerical results based on combined palynological and anatomical characters were discussed and compared with traditional taxonomic treatments. It was found that the midrib shape, mesophyll type, P/E (polar/equatorial), sulcus length, and lumina width are the most valuable traits in separating the examined taxa. The numerical analysis showed that Lilium candidum L. differs from the rest Turkish Lilium and also confirmed a close relationship between L. szovitsianum Fisch. & Avé-Lall. and L. armenum Miscz. ex Grossh. Also this study is the first report dealing with anatomical and palynological features of all Turkish lilies.

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References

  • Akman Y. & Ketenoğlu O. 1986. The climate and vegetation of Turkey. Proceedings of the Royal Society of Edinburgh-Section B. Biological Sciences 89: 123–134.

    Google Scholar 

  • Aktaş K., Baran P., Özdemir C. & Altan Y. 2009. Anatomical properties of endemic Lilium carniolicum Bernh. ex W. Koch var. artvinense (Miscz.) Davis & Henderson (Liliaceae) in Turkey. J. Sci. Technol. 3: 18–24.

    Google Scholar 

  • Baker J.G. 1871. A new synopsis of all the known lilies. Gard. Chron. 28: 104.

    Google Scholar 

  • Baranova M.V. 1985. Palynoderm ultrastructure and morphological types of pollen grains in the genus Lilium (Liliaceae). Bot. Žurn. 70: 297–304. (In Russian)

    Google Scholar 

  • Baranova M.V. 1988. A synopsis of the system of the genus Lilium (Liliaceae). Bot. Žurn. 73: 1319–1329.

    Google Scholar 

  • Ceter T., Ekici M., Pınar N.M. & Ozbek F. 2013. Pollen morphology of Astragalus L. section Hololeuce Bunge (Fabaceae) in Turkey. Acta Bot. Gallica 160: 43–52.

    Article  Google Scholar 

  • Clement C. & Audran J.C. 1995. Anther wall layers control pollen sugar nutrition in Lilium. Protoplasma 187: 172–181.

    Article  CAS  Google Scholar 

  • Comber H.F. 1949. A new classification of the genus Lilium. Lily Year-Book 13: 86–105.

    Google Scholar 

  • Coskuncelebi K. & Beyazoglu O. 1999. The anatomy of Lilium ciliatum P.H. Davis. In: International Symposium on Protection of Natural Environmental and Ehrami Karacam. 1999 Sept 23–25, 67: 799–806. Kütahya.

    Google Scholar 

  • Coskuncelebi K., Inceer H. & Beyazoglu O. 2005. Karyotypic variation in Lilium ssp. Bangl. J. Bot. 34: 85–89.

    Google Scholar 

  • Davis P.H. & Henderson D.M. 1984. Lilium L., vol. 8 pp. 279–284. In: Davis P.H. (ed), Flora of Turkey and the East Aegean Islands, Edinburgh University Press, Edinburgh.

    Google Scholar 

  • Dhyani A., Bahuguna Y.M., Semwal D.P., Nautiyal B.P. & Nautiyal M.C. 2009. Anatomical features of Lilium polyphyllum D. Don ex Royle (Liliaceae). J. Amer. Sci. 5(5): 85–90.

    Google Scholar 

  • Du Y., Wei C., Wang Z., Li S., He H. & Jia G. 2014. Lilium spp. pollen in China (Liliaceae): Taxonomic and phylogenetic implications and pollen evolution related to environmental conditions. Plos One 9(1): doi:10.1371/journal.pone.0087841.

    Google Scholar 

  • Endlicher S. 1840. Genera Plantarum Secundum Ordines Naturales Disposita. Fr. Beck, Wien.

    Google Scholar 

  • Erdtman G. 1952. Pollen Morphology and Plant Taxonomy. Angiosperms. M.A., Waltham.

    Google Scholar 

  • Furness C.A. & Rudall P.J. 2001. Pollen and anther characters in monocot systematics. Grana 40: 17–25.

    Article  Google Scholar 

  • Gao Y.D., Hohenegger M., Harris A.J., Zhou S.D., He X.J. & Wan J. 2012. A new species in the genus Nomocharis Franchet (Liliaceae): evidence that brings the genus Nomocharis into Lilium. Plant Syst. Evol. 298: 69–85.

    Article  Google Scholar 

  • Gämperle R. 1998. A new species of lily from Turkey. Quart. Bull. Alp. Gard. Soc. 66: 378–389.

    Google Scholar 

  • Hodgson J.G., Sharafi M., Jalili A., Diaz S., Montserrat-Marti G., Palmer C., Cerabolini B. et al. 2010. Stomatal vs. genome size in angiosperms: the somatic tail wagging the genomic dog. Ann. Bot. 105: 573–584.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • İkinci N. 2005. Revision of the genus Lilium (Liliaceae) in Turkey. PhD thesis, Abant İzzet Baysal University, Turkey.

    Google Scholar 

  • İkinci N. 2011. Molecular phylogeny and divergence times estimates of Lilium section Liriotypus (Liliaceae) based on plastid and nuclear ribosomal ITS DNA sequence data. Turk. J. Bot. 35: 319–330.

    Google Scholar 

  • İkinci N. 2012. Lilium L., pp. 609–610. In: Güner A., Aslan S., Ekim T., Vural M. & Babaç M.T. (eds), Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul.

    Google Scholar 

  • İkinci N., Oberprieler C. & Güner A. 2006. On the origin of European lilies: Phylogenetic analysis of Lilium section Liriotypus using sequences of the nuclear ribosomal transcribed spacers. Willdenowia 36: 647–656.

    Article  Google Scholar 

  • İkinci N. & Oberprieler C. 2010. Genetic relationship among NE Turkish Lilium L. (Liliaceae) species based on a random amplified polymorphic DNA analysis. Plant Syst. Evol. 284: 41–48.

    Article  Google Scholar 

  • Inceer H., Hayırlıoglu-Ayaz S. & Beyazoglu O. 2002. Karyological study on some taxa of the genus Lilium L. (Liliaceae). Pak. J. Bot. 34: 33–40.

    Google Scholar 

  • Kameshwari M.N.S. 2011. Pollen morphology in some members of Liliaceae. Int. J. Engineer. Sci. Technol. 3(5): 3825–3830.

    Google Scholar 

  • Kaviani B., Dehkaei M.N.P., Darabi A.H., Rafizadeh A. & Rahmati B. 2008. The anatomical properties of endemic Lilium ledebourii (Baker) Bioss. (Liliaceae) species. Int. J. Bot. 4: 62–66.

    Article  Google Scholar 

  • Kesselring W. 1932. Lilium monadelphum and its allies. Lily Year-Book 1: 66–71.

    Google Scholar 

  • Kim Y.S. & Lee W.B. 1990. A study of anatomical characters on the genus Lilium L. in Korea. Korean J. Plant Tax. 20: 179–189. (In Korean)

    Google Scholar 

  • Kosenko V.N. 1999. Contributions to the pollen morphology and taxonomy of the Liliaceae. Grana 38: 20–30.

    Article  Google Scholar 

  • Kuprianova L.A. 1983. Erythronium, Tulipa, Lillium, pp. 134, 139–141, 147–149. In: Spores of pteridophytes and pollen of gymnosperms and monocotyledons of the flora of the European Russia, Nauka, Leningrad.

    Google Scholar 

  • Lee C.S., Kim S.C., Yeau S.H. & Lee N.S. 2011. Major Lineages of the Genus Lilium (Liliaceae) Based on nrDNA ITS Sequences, with Special Emphasis on the Korean Species. J. Plant Biol. 54: 159–171

    Article  Google Scholar 

  • Lersten N.R. & Curtis J.D. 2001. Idioblasts and other unusual internal foliar secretary structures in Scrophulariaceae. Plant Syst. Evol. 227: 63–73.

    Article  Google Scholar 

  • Makbul S., Turkmen Z., Coskuncelebi K. & Beyazoglu O. 2008. Anatomical and pollen characters in the genus Epilobium L. (Onagraceae) from Northeast Anatolia. Acta Biol. Cracov. Bot. 50: 57–67.

    Google Scholar 

  • Mandenova I. 1940. Lilii Kavkaza: Caucasian lilies, vol 8: 149–208, Trudy Tbilis Bot. Inst.

    Google Scholar 

  • Masoumi S.M. 2012. Pollen morphology of Erythronium L. (Liliaceae) and its systematic relationships. J. Basic. Appl. Sci. Res. 2(2): 1833–1838.

    Google Scholar 

  • McRae E.A. 1998. Lilies, a Guide for Growers and Collectors. Timber Press, Portland.

    Google Scholar 

  • Muratović E., Bogunić F., Šoljan D. & Martin J. 2010a. Stomata and polen grain characteristics of two endemic lilies: Lilium bosniacum and L. carniolicum (Liliaceae). Phytol. Balcan. 16: 285–292.

    Google Scholar 

  • Muratović E., Hidalgo O., Garnatje T. & Siljak-Yakovlev S. 2010b. Molecular phylogeny and genome size in European lilies (Genus Lilium, Liliaceae). Adv. Sci. Lett. 3: 180–189.

    Article  Google Scholar 

  • Muratović E., Robin O., Bogunić F., Šoljan D. & Siljak-Yakovlev S. 2010c. Karyotype evolution and speciation of European lilies from Lilium sect. Liriotypus. Taxon 59: 165–175.

    Google Scholar 

  • Nishikawa T., Okazaki K., Arakawa K. & Nagamine T. 2001. Phylogenetic analysis of section Sinomartagon in genus Lilium using sequences of the internal transcribed spacer region in nuclear ribosomal DNA. Breed. Sci. 51: 39–46.

    Article  CAS  Google Scholar 

  • Özdemir C. 2003. Morphological, anatomical and cytological characteristics of endemic Lilium ciliatum P.H. Davis (Liliaceae) in Turkey. Pak. J. Bot. 35: 99–110.

    Google Scholar 

  • Özen F., Temeltaş H. & Aksoy Ö. 2012. The anatomy and morphology of the medicinal plant, Lilium candidum L. (Liliaceae), distributed in Marmara region of Turkey. Pak. J. Bot. 44: 1185–1192.

    Google Scholar 

  • Podani J. 1993. Multivariate Data Analysis in Ecology and Systematic, A Methodological Guide to Syn-Tax 5.0 Package SPB Academic Publishing, Netherlands.

    Google Scholar 

  • Punt W., Hoen P.P., Blackmore S., Nilsson S. & Le Thomas A. 2007. Glossary of pollen and spore terminology. Rev. Palaeobot. Palyno. 143: 1–81.

    Article  Google Scholar 

  • Pupuleku B., Kapidani G., Kallajxhiu N., Naqellari P. & Turku S. 2010. Palynological studies of pollen grains of Albania’s endemic plant Festucopsis serpentini (C.E. Hubbard) Melderis and Lilium martagon L. in three different habitats. Natura Montenegrina 9: 431–440.

    Google Scholar 

  • Rešetnik I., Liber Z., Satovic Z., Cigić P. & Nikolić T. 2007. Molecular phylogeny and systematics of the Lilium carniolicum group (Liliaceae) based on nuclear ITS sequences. Plant Syst. Evol. 265: 45–58.

    Article  Google Scholar 

  • Rønsted N., Law S., Thornton H., Fay M.F. & Chase M.W. 2005. Molecular phylogenetic evidence for the monophyly of Fritillaria and Lilium (Liliaceae; Liliales) and the infrageneric classification of Fritillaria. Mol. Phylogenet. Evol. 35: 509–527.

    Article  PubMed  Google Scholar 

  • Sneath P.H.A. & Sokal R.R. 1973. Numerical Taxonomy: The Principles and Practice of Numerical Classification.W.H. Freeman and Company, San Francisco.

    Google Scholar 

  • Tekşen M., Aytaç Z. & Pınar N.M. 2010. Pollen morphology of the genus Fritillaria L. (Liliaceae) in Turkey. Turk. J. Bot. 34: 397–416.

    Google Scholar 

  • Troia A., Raimondo F.M., Castellano G. & Spadaro V. 2012. Morphological, karyological and taxonomic remarks on Ferulago nodosa (L.) Boiss. (Apiaceae). Plant Biosyst. 146: 330–337.

    Article  Google Scholar 

  • Vardar Y. 1987. Botanikte Preparasyon Tekniği. Ege Üniversitesi, Fen Fakültesi Yayınlar, İzmir.

    Google Scholar 

  • Wilson E.H. 1925. The Lilies of Eastern Asia, A Monograph. Dulau and Company Ltd., London.

    Google Scholar 

  • Woodcock H.B.D. & Stearn W.T. 1950. Lilies of the World: Their Cultivation and Classification. Country Life, London.

    Google Scholar 

  • Yentür S. 2003. Bitki Anatomisi. İstanbul Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, İstanbul.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Faculty of Sciences and Arts, Recep Tayyip Erdoğan University, Rize, Turkey

    Seher Güven, Seda Okur & Serdar Makbul

  2. Department of Biology, Science Faculty, Karadeniz Technical Universty, Trabzon, Turkey

    Mine Sezen Demırel & Kamil Coskuncelebi

  3. Department of Biomedical Engineering, Faculty of Engineering and Architecture, Yeni Yüzyıl University, İstanbul, Turkey

    Osman Beyazoğlu

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  1. Seher Güven
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  2. Seda Okur
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  3. Mine Sezen Demırel
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  4. Kamil Coskuncelebi
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  5. Serdar Makbul
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  6. Osman Beyazoğlu
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Correspondence to Seda Okur.

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Güven, S., Okur, S., Demırel, M.S. et al. Pollen morphology and anatomical features of Lilium (Liliaceae) taxa from Turkey. Biologia 69, 1122–1133 (2014). https://doi.org/10.2478/s11756-014-0416-2

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  • DOI: https://doi.org/10.2478/s11756-014-0416-2

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