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Comprehensive characterization of human adipose tissue-derived stem cells expanded in vitro

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Abstract

Adipose tissue seems to be a rich and safe source of mesenchymal stem cells (MSCs). The present study was aimed to investigate the biological and morphological characteristics of human adipose tissue-derived stem cells (ATSCs). Light and transmission electron microscopy were used. Course of proliferation was analyzed by growth curve. Expression of surface antigens was assessed by flow cytometry. Chondrogenic potential was assessed by immunohistochemistry. Obtained results showed morphology typical of fibroblastoid cells. TEM analysis proved ultrastructural morphology similar to MSCs from other sources. ATSCs reflected their proteosynthetic and metabolic activity. Each cell had irregular shape of nucleus with noticeable nucleoli. Abundant cisterns of rough endoplasmic reticulum were present in their cytoplasm. Karyotype mapping showed normal count of human chromosomes (46,XX). The growth curve revealed high capability for proliferation and population doubling time was 27.36 hours. ATSCs were positive for CD13, CD29, CD44, CD73, CD90, CD105 and CD106, but did not express CD14, CD34, CD45 and HLA-DR. It was also proved that ATSCs underwent chondrogenic differentiation in vitro. On the basis of obtained results it should be emphasized that ATSCs are typical MSCs and after further investigations they may be used in tissue engineering and regenerative medicine.

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Authors and Affiliations

  1. Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University, Sasinkova 4, SK-811 08, Bratislava, Slovakia

    Ľuboš Danišovič, Marcela Kuniaková, Zuzana Varchulová-Nováková & Daniel Böhmer

  2. Clinic of Plastic Surgery, Faculty of Medicine, Comenius University, Pažítková 4, SK-821 01, Bratislava, Slovakia

    Martin Boháč

  3. Institute of Histology and Embryology, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08, Bratislava, Slovakia

    Martin Boháč, Ivan Varga, Jana Rusnáková & Štefan Polák

  4. Institute of Histology and Embryology, Faculty of Medicine, Slovak Medical University, Limbova 12, SK-833 03, Bratislava, Slovakia

    Ivan Varga

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  1. Ľuboš Danišovič
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  2. Marcela Kuniaková
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  3. Zuzana Varchulová-Nováková
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Correspondence to Ľuboš Danišovič.

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Danišovič, Ľ., Kuniaková, M., Varchulová-Nováková, Z. et al. Comprehensive characterization of human adipose tissue-derived stem cells expanded in vitro . Biologia 68, 747–753 (2013). https://doi.org/10.2478/s11756-013-0201-7

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  • DOI: https://doi.org/10.2478/s11756-013-0201-7

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