Skip to main content
SpringerLink
Log in

Changes in photosynthetic apparatus of mustard (Sinapis alba) cotyledons following cycloheximide and kinetin treatments

  • Published:
Biologia Aims and scope Submit manuscript

Abstract

Biochemical and accompanying structural characteristics of the photosynthetic process were studied in mustard seedlings cultivated on medium with increasing concentrations of cycloheximide alone as well as in combination with various kinetin concentrations. After 7 days of cultivation the contents of total chlorophyll, carotenoids and content of Rubisco in mustard cotyledons were determined. The content of chlorophyll pigments and carotenoids decreased in dependence of cycloheximide concentration. Following antibiotic treatment the content of both Rubisco subunits markedly decreased. In addition cycloheximide caused disturbance in mesophyll organization and chloroplast ultrastructure.

Kinetin applied with cycloheximide increased the amount of photosynthetic pigments as well as of Rubisco, compared to the cycloheximide alone. In the seedlings treated with cycloheximide+kinetin the structure of leaf mesophyll and chloroplast membrane system was similar to control. Our results indicate that kinetin diminished the negative effects of cycloheximide on photosynthetic pigments and Rubisco as well as on the structural traits of the cotyledons.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aliferis K.A. & Chrysayi-Tokousbalides M. 2005. On the mode of action of the phytotoxin (8R,16R)-(−)-pyrenophorin. Pestic. Biochem. Phys. 86: 7–14.

    Article  CAS  Google Scholar 

  • Arteca R.N. 1996. Plant growth substances. Chapman & Hall Press, New York, 332 pp.

    Google Scholar 

  • Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal. Biochem. 72: 248–254.

    Article  PubMed  CAS  Google Scholar 

  • Costa M.L., Civello P.M., Chaves A.R. & Martines G.A. 2005. Effect of ethephon and 6-benzylaminopurine on chlorophyll degrading enzymes and a peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica oleracea L.) at 20°C. Postharvest Biol. Tec. 35: 191–199.

    Article  CAS  Google Scholar 

  • Folly P. & Engel N. 1999. Chlorophyll b to chlorophyll a conversion precedes chlorophyll degradation in Hordeum vulgare L. J. Biol. Chem. 274: 21811–21816.

    Article  PubMed  Google Scholar 

  • Hanten J.J. & Pierce S.K. 2001. Synthesis of several light-harvesting complex I polypeptides is blocked by cyclohex-imide in symbiotic chloroplasts in the sea slug, Elysia chlorotica (Gould): a case for horizontal gene transfer between alga and animal? Biol. Bull. 201: 34–44.

    Article  PubMed  CAS  Google Scholar 

  • Harms J., Schluenzen F., Zarivach R., Bashan A., Gat S., Agmon I., Bartels H., Franceschi F. & Yonath A. 2001. High resolution structure of the large ribosomal subunit from a mesophilic eubacterium. Cell 107: 679–688.

    Article  PubMed  CAS  Google Scholar 

  • Hudák J., Baloun J. & Baluška F. 1990. Plastid senescence 3. Changes of chloroplast structure induced by antibiotics. Acta Fac. Rerum Phy. 25: 43–50.

    Google Scholar 

  • Hudák J., Herich R. & Bobák M. 1983. Plastidy. Veda, Bratislava, 152 pp.

    Google Scholar 

  • Hudák J., Vizárová G., Šikulová J. & Ovečková O. 1996. Effect of cytokinins produced by strains of Agrobacterium tumefaciens with binary vectors on plastids in senescent barley leaves. Acta Physiol. Plant. 18: 205–210.

    Google Scholar 

  • Karnovsky M.J. 1965. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27: 137A–138A.

    Google Scholar 

  • Kaup M.T., Froese C.D. & Thompson J.E. 2002. A role for diacylglycerol acyltransferase during leaf senescence. Plant Physiol. 129: 1616–1626.

    Article  PubMed  CAS  Google Scholar 

  • Kirk J.T.O. & Tilney-Bassett R.A.E. 1978. The Plastids. Elsevier/North-Holland Biomedical Press, Amsterdam, 960 pp.

    Google Scholar 

  • Kuroda H., Masuda T., Fusada N., Ohta H., Shioi Y. & Takamiya K. 2000. Expression of NADPH-protochlorophyllide oxidore-ductase gene in fully greened leaves of cucumber. Plant Cell Physiol. 41: 226–229.

    PubMed  CAS  Google Scholar 

  • Laemmli U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.

    Article  PubMed  CAS  Google Scholar 

  • Lichtenthaler H.K. 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Method. Enzymol. 148: 350–382.

    Article  CAS  Google Scholar 

  • Lux A. 1981. Rýchla metóda farbenia polotenkých rezov z rastlinných objektov. Biológia 36: 753–757.

    Google Scholar 

  • Machlicová A., Slováková L’. & Hudák J. 2005. Regeneration of degenerated plastids by erythromycin in mustard cotyledons after kinetin influence. Acta Physiol. Plant. Supplement 27: 21–22.

    Google Scholar 

  • Maniatis T., Fritsch E.K. & Sambrook J. 1989. Molecular cloning: A laboratory manual. Cold Spring Harbor, New York, pp. 1847–1859.

    Google Scholar 

  • Martin C. & Thimann K.V. 1972. The role of protein synthesis in the senescence of leaves. The formation of protease. Plant Physiol. 49: 64–71.

    PubMed  CAS  Google Scholar 

  • Mellis A. 1991. Dynamics of photosynthetic membrane composition and function. Biochim. Biophys. Acta 1058: 87–106.

    Article  Google Scholar 

  • Metwally A., Tsonev T. & Zeinalov Y. 1997. Effect of cytokinins on the photosynthetic apparatus in water-stressed and rehydrated bean plants. Photosynthetica 34: 563–567.

    Article  CAS  Google Scholar 

  • Mok D.W.S. & Mok M.C. 2001. Cytokinin metabolism and action. Annu. Rev. Plant Phys. 52: 89–118.

    Article  CAS  Google Scholar 

  • Naik G.R., Mukherjee I. & Reid D.M. 2002. Influence of cytokinins on the methyljasmonate-promoted senescence in Helianthus annuus cotyledons. Plant Growth Regul. 38: 61–68.

    Article  CAS  Google Scholar 

  • Naito K., Ikeda K., Saito T., Suzuki H., Yamada M. & Kuraishi S. 1980. Importance of RNA synthesis within the lag phase preceding benzyladenine-induced growth of cucumber cotyledons in the dark. Plant Cell Physiol. 21: 1419–1430.

    CAS  Google Scholar 

  • Niwa Y., Kato T., Tabata S., Seki M., Kobayashi M., Shinozaki K. & Moriyasu Y. 2004. Disposal of chloroplasts with abnormal function into the vacuole in Arabidopsis thaliana cotyledon cells. Protoplasma 223: 229–232.

    Article  PubMed  Google Scholar 

  • Peterson L.W. & Huffaker R.C. 1975. Loss of ribulose 1,5-diphosphate carboxylase and increase in proteolytic activity during senescence of detached primary barley leaves. Plant Physiol. 55: 1009–1015.

    PubMed  CAS  Google Scholar 

  • Pjon Ch.J., Kim Y.M. & Pak J.Y. 1992. Interaction of cycloheximide and light on chlorophyll content and chloroplast ultrastructure in Brassica campestris. J. Plant Res. 105: 681–685.

    CAS  Google Scholar 

  • Salopek-Sondi B., Kovač M., Prebeg T. & Magnus W. 2002. Developing fruit direct post-floral morphogenesis in Helleborus niger L. J. Exp. Bot. 375: 1949–1957.

    Article  Google Scholar 

  • Sanita di Toppi L., Vurro E., Rossi L., Marabottini R., Musetti R., Careri M., Maffini M., Mucchino C., Corradini C. & Badiani M. 2007. Different compensatory mechanisms in two metal-accumulating aquatic macrophytes exposed to acute cadmium stress in outdoor artificial lakes. Chemosphere 68: 769–780.

    Article  PubMed  CAS  Google Scholar 

  • Schmülling T., Schäfer S. & Romanov G. 1997. Cytokinins as regulators of gene expression. Physiol. Plant. 100: 505–519.

    Article  Google Scholar 

  • Tenson T. & Mankin A. 2006. Antibiotics and the ribosome. Mol. Microbiol. 59: 1664–1677.

    Article  PubMed  CAS  Google Scholar 

  • Voicu M.C. & Zwiazek J.J. 2003. Cycloheximide inhibits root water flow and stomatal conductance in aspen (Populus tremuloides) seedlings. Plant Cell Environ. 28: 199–208.

    Google Scholar 

  • Wildman R.B. & Hunt P. 2005. Phytoferritin associated with yellowing in leaves of Cocos nucifera (Arecaceae). Protoplasma 87: 121–134.

    Article  Google Scholar 

  • Wingler A., von Schaewen A., Leegood R.C., Lea P.J. & Qick W.P. 1998. Regulation of leaf senescence by cytokinin, sugars and light. Effects on NADH-dependant hydroxypyruvate reductase. Plant Physiol. 116: 329–335.

    Article  CAS  Google Scholar 

  • Wittenbach V.A, Lin W. & Hebert R. 1982. Vacuolar localization of proteases and degradation of chloroplasts in mesophyll protoplasts from senescing primary wheat leaves. Plant Physiol. 69: 98–102.

    Article  PubMed  CAS  Google Scholar 

  • Yagisawa F., Mori, T., Higashiyama T., Kuroiwa H. & Kuroiwa T. 2003. Regulation of Brassica rapa chloroplast proliferation in vivo and in cultured leaf discs. Protoplasma 222: 139–148.

    Article  PubMed  CAS  Google Scholar 

  • Yaronskaya E.B., Gritskevich E.R., Trukhanovets N.L. & Averina N.G. 2007. Effect of kinetin on early stages of chlorophyll biosynthesis in streptomycin-treated barley seedlings. Russ. J. Plant Phys. 54: 388–395.

    Article  CAS  Google Scholar 

  • Zavaleta-Mancera H.A., Thomas B.J., Thomas H. & Scott I.M. 1999. Regreening of senescent Nicotiana leaves. J. Exp. Bot. 50: 1683–1689.

    Article  CAS  Google Scholar 

  • Zechleder V., Bišová K., Vítová M., Kubín Š. & Hendrzchová J. 2002. Variety of cell cycle patterns in the alga Scenedesmus quadricauda (Chlorophyta) as revealed by application of illumination regimes and inhibitors. Eur. J. Phycol. 37: 361–371.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 14, SK-84523, Bratislava, Slovakia

    Adriana Machlicová

  2. Department of Plant Physiology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina B-2, SK-84215, Bratislava 4, Slovakia

    Adriana Machlicová, L’udmila Slováková & Ján Hudák

Authors
  1. Adriana Machlicová
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L’udmila Slováková
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ján Hudák
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Adriana Machlicová.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Machlicová, A., Slováková, L. & Hudák, J. Changes in photosynthetic apparatus of mustard (Sinapis alba) cotyledons following cycloheximide and kinetin treatments. Biologia 63, 664–669 (2008). https://doi.org/10.2478/s11756-008-0128-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2478/s11756-008-0128-6

Key words

Search

Navigation