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Phylogenetic relationships of species in Pseudoroegneria (Poaceae: Triticeae) and related genera inferred from nuclear rDNA ITS (internal transcribed spacer) sequences

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Abstract

To evaluate the phylogenetic relationships of species in Pseudoroegneria and related genera, the nuclear ribosomal internal transcribed spacer (ITS) sequences were analyzed for eighteen Pseudoroegneria (St), two Elytrigia (E e St), two Douglasdeweya (StP), three Lophopyrum (E e and E b), three Agropyron (P), two Hordeum (H), two Australopyrum (W) and two Psathyrostachys (Ns) accessions. The main results were: (i) Pseudoroegneria gracillima, P. stipifolia, P. cognata and P. strigosa (2x) were in one clade, while P. libanotica, P. tauri and P. spicata (2x) were in the other clade, indicating there are the differentiations of St genome among diploid Pseudoroegneria species; (ii) P. geniculata ssp. scythica, P. geniculata ssp. pruinifera, Elytriga caespitosa and Et. caespitosa ssp. nodosa formed the E e St clade with 6-bp indel in ITS1 regions; and (iii) Douglasdeweya wangii, D. deweyi, Agropyron cristatum and A. puberulum comprised the P clade. It is unreasonable to treat P. geniculata ssp. scythica and P. geniculata ssp. pruinifera as the subspecies of P. geniculata, and they should be transferred to a new genus Trichopyrum, which consists of species with E e St genomes. It is also suggested that one of the diploid donor of D. wangii and D. deweyi is derived from Agropyron species, and it is reasonable to treat tetraploid species with StP genomes into Douglasdeweya.

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Abbreviations

BI:

Bayesian inference

bp:

base pairs

ITS:

internal transcribed spacer

ML:

maximum likelihood

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Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, 611830, Sichuan, People’s Republic of China

    Haiqing Yu, Xing Fan, Chun Zhang & Yonghong Zhou

  2. Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Yaan, 625014, Sichuan, People’s Republic of China

    Haiqing Yu & Yonghong Zhou

  3. College of Biology and Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, People’s Republic of China

    Chunbang Ding & Xiaoli Wang

Authors
  1. Haiqing Yu
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  2. Xing Fan
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  3. Chun Zhang
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  4. Chunbang Ding
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  5. Xiaoli Wang
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  6. Yonghong Zhou
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Correspondence to Yonghong Zhou.

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Yu, H., Fan, X., Zhang, C. et al. Phylogenetic relationships of species in Pseudoroegneria (Poaceae: Triticeae) and related genera inferred from nuclear rDNA ITS (internal transcribed spacer) sequences. Biologia 63, 498–505 (2008). https://doi.org/10.2478/s11756-008-0091-2

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