Abstract
Jatropha curcas is an oil bearing species with multiple uses and considerable economic potential as a biofuel crop. The effect of NaCl stress on growth, ion accumulation, contents of protein, proline, and antioxidant enzymes activity in callus cultures of J. curcas was investigated. Exposure of callus to NaCl decreased growth in a concentration dependent manner. NaCl treated callus accumulated Na and declined in K, Ca and Mg contents. Na/K ratio increased steadily as a function of external NaCl treatment. NaCl induced significant differences in quality and quantity of proteins, whereas, proline accumulation remained more or less constant with treatment. NaCl stress enhanced the activity of superoxide dismutase (SOD; E.C. 1.15.1.1) and peroxidase (POX; E.C. 1.11.1.7). Further in the isoenzyme studies, four SOD isoenzymes (SOD 1, 2, 3, and 4) and two POX isoenzymes (POX 1 and 2) were detected with the treatment. NaCl strongly induced activity of SOD 4 isoenzyme in 40, 60, 80 mM and POX 2 isoenzyme in 40 and 80 mM NaCl concentrations. Increase in antioxidant enzymes activity could be a response to cellular damage induced by NaCl. This increase could not stop the deleterious effects of NaCl, but it reduced stress severity and thus allowed cell growth to occur.
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Apse M.P., Aharon G.S., Snedden W.A. & Blumwald E. 1999. Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis. Science 285: 256–1258.
Bajji M., Kinet J.M. & Lutts S. 1998. Salt stress on roots and leaves of Atriplex halimus L. and their corresponding callus culture. Plant Sci. 137: 131–142.
Bates L.R., Waldren R.P. & Teare I.D. 1973. A rapid determination of free proline for water stress studies. Plant Soil 39: 205–207.
Beauchamp C.O. & Fridovich I. 1971. Superoxide dismutase; improved assays and an assay applicable to acrylamide gels. Ann. Biochem. 44: 276–287.
Bradford M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
Cherian S. & Reddy M.P. 2003. Evaluation of NaCl tolerance in callus cultures of Suaeda nudiflora Moq. Biol. Plant. 46: 193–198.
Cramer G.R., Lauchli A. & Polito A. 1985. Displacement of Ca by Na from the plasmalemma of root cells. A primary response to salt stress. Plant Physiol. 79: 207–211.
Damerval C., Le Guilloux M., Blaisonneau J. & de Vienne D. 1987. A simplification of Heukeshoven and Dernick’s silver staining of proteins. Electrophoresis 8: 158–159
Elkahoui S., Hernandez J.A., Abdelly C., Ghrir R. & Limam F. 2005. Effects of salt on lipid peroxidation and antioxidant enzyme activities of Catharanthus roseus suspension cells. Plant Sci. 168: 607–613.
Lacerda C.F., Cambraia J., Cano M.A.O. & Ruiz H.A. 2001. Plant growth and solute accumulation and distribution in two sorghum genotypes under NaCl stress. Rev. Bras. Fisiol. Veg. 13: 270–284.
Francis G., Edinger R. & Becker K. 2005. A concept for simultaneous wasteland reclamation, fuel production, and socioeconomic development in degraded areas in India. Need, potential and perspectives of Jatropha plantations. Nat. Resources Forum 29: 12–24.
Giannopolitis C.N. & Ries S.K. 1977. Superoxide dismutase occurrence in higher plants. Plant Physiol. 59: 309–314.
Gulati A. 1989. Enzyme activity and isoenzyme patterns of Coccinia grandis in relation to sex expression. Curr. Sci. 58: 64–68.
Hall J.L. & Flowers T.J. 1973. The effect of salt on protein synthesis in the halophytes Suaeda maritime. Planta 110: 361–368.
Hernandez J.A., Jimenez A., Mullineaux P.M. & Sevilla F. 2000. Tolerance of pea (Pisum sativum L.) to long term salt stress is associated with induction of antioxidant defenses. Plant Cell Environ. 23: 853–862.
Laemmli U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.
Maggio A., Reddy M.P. & Joly R.J. 2000. Leaf gas exchange and solute accumulation in the halophyte Salvadora persica grown at moderate salinity. Environ. Exp. Bot. 44: 31–38.
McCoy T.J. 1987. Tissue culture evaluation of NaCl tolerance in Medicago sativa species: cellular versus whole plant response. Plant Cell Rep. 6: 31–34.
Meloni D.A. Gulotta M.R., Martinez, C.A. & Olive M.A. 2004. The effect of salt stress on growth, nitrate reduction and proline and glycinebetaine accumulation in Prosopis alba. Braz. J. Plant Physiol. 16: 39–46.
Misra H.P. & Fridovich I. 1977. Superoxide dismutase and peroxidase: A positive activity stain applicable to polyacrylamide gel electropherograms. Arch. Biochem. Biophys. 183: 511–515.
Murashige T. & Skoog F.A. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473–497.
Niknam V., Razavi N., Ebrahimzadeh H. & Sharifizadeh B. 2006. Effect of NaCl on biomass, protein and proline contents, and antioxidant enzymes in seedling and calli of two Trigonella species. Biol. Plant. 50: 591–596.
Petrusa L.M. & Winicov I. 1997. Proline status in salt-tolerant and salt-sensitive alfalfa cell lines and plants in response to NaCl. Plant Physiol. Biochem. 35: 303–310.
Piqueras A., Hernandez J.A., Olmos E., Hellin E. & Sevilla F. 1996. Changes in antioxidant (CT) enzymes and organic solutes associated with adaptation of citrus cells to salt stress. Plant Cell Tiss. Org. Culture 45: 53–60.
Reddy M.P. & Iyengar E.R.R. 1999. Crop responses to salt stress: Seawater application and prospects, pp. 1041–1068. In: Pessarakli M.M. (ed.), Handbook of Plant and Crop Stress. Marcel Dekker In. New York.
Shannon L.M., Key E. & Lew J.Y. 1966. Peroxidase isoenzymes from horse radish roots: Isolation and physiological properties. J. Biol. Chem. 241: 2166–2172.
Shibli R.A., Kushad M., Yousef G.G. & Lila M.A. 2007. Physiological and biochemical responses of tomato microshoots to induced salinity stress with associated ethylene accumulation. Plant Growth Regul. 51: 159–169
Shibli R.A., Sawwan J., Swaidat I. & Tahat M. 2001. Increased phosphorus mitigates the adverse effects of salinity in tissue culture. Comm. Soil Sci. Plant Anal. 32: 429–440.
Stewart G.R. & Lee J.A. 1974. The role of proline accumulation in halophytes. Planta 20: 279–289.
Takeda Y. 1982. Development study on Jatropha curcas (sabudum) oil as a substitute for diesel engine oil in Thailand. J. Agri. Assoc. 120: 1–8.
Uprety D.C. & Sarin M.N. 1976. Physiological studies on salt tolerance in Pisum sativum L. IV, Ionic composition and nitrogen metabolism. Acta Agron. Hung. 25: 455–460.
Zhu J.K. 2001. Plant salt tolerance. Trends Plant Sci. 6: 66–71.
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Kumar, N., Pamidimarri, S.D.V.N., Kaur, M. et al. Effects of NaCl on growth, ion accumulation, protein, proline contents and antioxidant enzymes activity in callus cultures of Jatropha curcas . Biologia 63, 378–382 (2008). https://doi.org/10.2478/s11756-008-0054-7
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DOI: https://doi.org/10.2478/s11756-008-0054-7