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Production of SacI and SacII isoschizomers by soil streptomycetes

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Abstract

Five fresh soil Streptomyces spp. strains were isolated, phylogenetically characterized on the basis of 16S rDNA sequences and analyzed for the presence of restriction modification systems. Three type II site-specific endonucleases were detected and partially purified. Two isolated enzymes were isoschizomers of SacI restriction endonuclease recognizing 5′-GAGCTC-3′ sequence; the third one recognised 5′-CCGCGG-3′ sequence and it was an isoschizomer of SacII. SacII like modification was observed in other two isolates having no detectable restriction activity. The lack of correlation between restriction and modification phenotypes and phylogenetic classification of the isolates indicates efficient gene transfer mechanism in the Streptomyces genus.

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Authors and Affiliations

  1. Department of Biochemistry, Institute of Chemistry, Faculty of Science, P. J. Safarik University, Moyzesova 11, SK-04001, Kosice, Slovakia

    Timea Tothova

  2. Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, SK-84551, Bratislava, Slovakia

    Andrej Godany

  3. Institute of Animal Physiology, Slovak Academy of Sciences, Soltesovej 4–6, SK-04001, Kosice, Slovakia

    Peter Javorsky & Peter Pristas

Authors
  1. Timea Tothova
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  2. Andrej Godany
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  3. Peter Javorsky
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  4. Peter Pristas
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Correspondence to Peter Pristas.

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Tothova, T., Godany, A., Javorsky, P. et al. Production of SacI and SacII isoschizomers by soil streptomycetes. Biologia 62, 381–385 (2007). https://doi.org/10.2478/s11756-007-0095-3

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  • DOI: https://doi.org/10.2478/s11756-007-0095-3

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