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Role of antioxidant vitamins administration on the oxidative stress

  • Research Article
  • Published:
Central European Journal of Medicine

Abstract

The health-promoting effects of antioxidant vitamins C and E supplementation are unclear. This study investigated the effects of vitamins C and E on the activities of reactive oxygen species (ROS)-scavenging enzymes and protein and lipid peroxidation statuses under resting and exercise-induced conditions. Thirteen healthy, previously untrained males (age 20–21 years) participated in this study. Seven subjects performed physical exercise using a cycle ergometer, and six performed a 6-min walk test (6MWT) prior to vitamin administration and after 1-week oral administration of vitamin C (1000 mg/day) and vitamin E (300 IU/day). Venous blood samples were collected before and after exercise. Plasma vitamin C concentration, superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity, and protein carbonyl and thiobarbituric acid-reactive substance (TBARS) contents were measured. Antioxidant supplementation increased vitamin C concentration by 34% (p<0.05), decreased SOD activity by 17% (p<0.05), increased GPx activity by 13% (p<0.05), and increased the GPx/SOD activity ratio by 37% (p<0.05). Protein carbonyl and TBARS contents were unaffected. Antioxidant vitamins effectively increase the plasma GPx/SOD activity ratio, but fail to reduce protein carbonyl levels induced by exercise.

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Authors and Affiliations

  1. Department of Rehabilitation, Kobe International University, 9-1-6, Koyocho-naka, Higashinada, Kobe, 658-0032, Japan

    Miki Tomoeda & Masahito Murakami

  2. Departments of Pathology, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, Higashinari, Osaka, 537-8511, Japan

    Miki Tomoeda, Chiaki Kubo, Hidenori Yoshizawa, Michiko Yuki, Masanori Kitamura, Shigenori Nagata, Yasuko Nishizawa & Yasuhiko Tomita

  3. Departments of Clinical laboratory, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, Higashinari, Osaka, 537-8511, Japan

    Yasuko Nishizawa

Authors
  1. Miki Tomoeda
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  2. Chiaki Kubo
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  3. Hidenori Yoshizawa
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  9. Yasuhiko Tomita
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Correspondence to Yasuhiko Tomita.

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Tomoeda, M., Kubo, C., Yoshizawa, H. et al. Role of antioxidant vitamins administration on the oxidative stress. cent.eur.j.med 8, 509–516 (2013). https://doi.org/10.2478/s11536-013-0173-6

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  • DOI: https://doi.org/10.2478/s11536-013-0173-6

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