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Hes-1-targeting siRNA inhibits the maturation of murine myeloid-derived dendritic cells

  • Research Article
  • Published:
Central European Journal of Biology

Abstract

Activation of Notch by Jagged-1 may plays a pivotal role in maturation of dendritic cells (DCs), but the mechanism has not been completely defined. In the present study, Hes-1 (Hairy/enhancer-of-split)-targeting siRNA was used to confirm a role of Jagged-1-Notch signaling pathway activation in maturation of murine bone marrow-derived DCs and to search for a target that plays a critical role. The results showed that compared with the control, lipopolysaccharide or Zymosan A groups, Jagged-1 (a soluble Jagged 1/Fc chimera protein) effectively increased expression of Hes-1 and Deltex-1 mRNA, which could be reversed by DAPT (2, 4-diamino-5-phenylthiazole), a specific inhibitor of the Notch signaling pathway. Hes-1-targeting siRNA could successfully down-regulate the endogenous Hes-1 expression in the DCs. Concurrently, a significant down-regulation of CD40, CD80, CD86 and MHC-II expressions on the surface of the DCs was found with the reduction of IL-12 yielded by the DCs. Our results demonstrate that Hes-1-targeting siRNA can inhibit the maturation of the DCs induced by Jagged-1, indicating Hes-1 may be an important target of Notch signaling mediating the maturation of DCs.

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Authors and Affiliations

  1. Institute of Tissue Transplantation and Immunology, Department of Immunobiology, Jinan University, 510632, Guangzhou, China

    Guoliang Chen, Chunyan Yu, Feiyue Xing, Pengtao You, Jingfang Di & Shan Zeng

  2. Department of Stomatology, Jinan University, 510632, Guangzhou, China

    Jing Liu

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  1. Guoliang Chen
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  2. Chunyan Yu
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  3. Feiyue Xing
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  4. Pengtao You
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  5. Jingfang Di
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  6. Shan Zeng
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  7. Jing Liu
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Correspondence to Feiyue Xing or Jing Liu.

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Chen, G., Yu, C., Xing, F. et al. Hes-1-targeting siRNA inhibits the maturation of murine myeloid-derived dendritic cells. cent.eur.j.biol. 8, 1102–1111 (2013). https://doi.org/10.2478/s11535-013-0231-8

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  • DOI: https://doi.org/10.2478/s11535-013-0231-8

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