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In Vitro Immunological Effects of CXCR3 Inhibitor AMG487 on Dendritic Cells

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Archivum Immunologiae et Therapiae Experimentalis Aims and scope

Abstract

AMG 487 is the targeted blocker of chemokine receptor CXCR3 and improves inflammatory symptoms by blocking the inflammatory cycle. Here we investigated whether AMG 487 affects dendritic cell (DC) biology and function. The expression of co-stimulatory markers on DCs was reduced, indicating the semi-mature state of DC when AMG 487 was added throughout the in vitro differentiation period. Additionally, when added solely during the final lipopolysaccharide-induced activation step, AMG 487 inhibited DC activation, as demonstrated by a decreased expression of activation markers. AMG487 also promoted the expression of PD-L2 and impaired the ability to induce antigen-specific T cell responses. Our results demonstrated that AMG 487 significantly affects DC maturity in vitro and function leading to impaired T cell activation, inducing DCs to have characteristics similar to tolerogenic DCs. AMG 487 may directly play an immunomodulatory role during DC development and functional shaping.

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Acknowledgements

We are grateful to Rhoda and Ed Perozzi for providing language help of this article.

Funding

This work was supported by the National Natural Science Foundation of China [No. 81570160 & No. 8197160].

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Authors and Affiliations

  1. Department of Hematology, Peking University First Hospital, 8 Xishiku Street, Xicheng District, Beijing, 100034, China

    Chenchen Qin, Huihui Liu, Bo Tang, Min Cao, Zhengyu Yu, Beichen Liu, Wei Liu, Yujun Dong & Hanyun Ren

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  1. Chenchen Qin
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  2. Huihui Liu
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  3. Bo Tang
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  4. Min Cao
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  7. Wei Liu
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Correspondence to Hanyun Ren.

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Qin, C., Liu, H., Tang, B. et al. In Vitro Immunological Effects of CXCR3 Inhibitor AMG487 on Dendritic Cells. Arch. Immunol. Ther. Exp. 68, 11 (2020). https://doi.org/10.1007/s00005-020-00577-3

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