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Moderate intermittent hypoxia/hyperoxia: implication for correction of mitochondrial dysfunction

  • Research Article
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Central European Journal of Biology

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Abstract

The purpose of this study was to appreciate the acute hypoxia-induced mitochondrial oxidative damage development and the role of adaptation to hypoxia/hyperoxia (H/H) in correction of mitochondrial dysfunction. It was demonstrated that long-term sessions of moderate H/H [5 cycles of 5 min hypoxia (10% O2 in N2) alternated with 5 min hyperoxia (30% O2 in N2) daily for two weeks]_attenuated basal and Fe2+/ascorbate-induced lipid peroxidation (LPO) as well as production of carbonyl proteins and H2O2 in liver mitochondria of rats exposed to acute severe hypoxia (7% O2 in N2, 60 min) in comparison with untreated animals. It was shown that H/H increases the activity of glutathione peroxidase (GPx), reduces hyperactivation of Mn-SOD, and decreases Cu,Zn-SOD activity as compared with untreated rats. It has been suggested that the induction of Mn-SOD protein expression and the coordinated action of Mn-SOD and GPx could be the mechanisms underlying protective effects of H/H, which promote the correction of the acute hypoxia-induced mitochondrial dysfunction. The increase in Mn-SOD protein synthesis without changes in Mn-SOD mRNA level under H/H pretreatment indicates that the Mn-SOD activity is most likely dependent on its posttranslational modification or on the redox state of liver mitochondria.

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Authors and Affiliations

  1. Department of Hypoxic States, Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine, 01024, Kiev, Ukraine

    Olga A. Gonchar & Irina N. Mankovska

Authors
  1. Olga A. Gonchar
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  2. Irina N. Mankovska
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Correspondence to Olga A. Gonchar.

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Gonchar, O.A., Mankovska, I.N. Moderate intermittent hypoxia/hyperoxia: implication for correction of mitochondrial dysfunction. cent.eur.j.biol. 7, 801–809 (2012). https://doi.org/10.2478/s11535-012-0072-x

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  • DOI: https://doi.org/10.2478/s11535-012-0072-x

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