Skip to main content
SpringerLink
Log in

Use of multivariate statistical techniques to optimize the separation of isoflavones by liquid chromatography

  • Short Communication
  • Published:
Central European Journal of Chemistry

Abstract

The aim of this paper is to optimize and validate a high performance liquid chromatography (HPLC) method for separation and quantification of five isoflavones. A statistical central composite design was used to separate all peaks. These multivariate procedures were efficient in determining the optimal separation condition using resolution, capacity factor, asymmetry and number of theoretical plates.

The effective separation of the examined compounds was applied on a Develosil RP Aqueous AR 5 RP-30 column with a gradient mobile phase system and a DAD detector.

The isolation and preconcentration of the isoflavones from urine and plasma samples were conducted by means of the solid-phase extraction (SPE). For optimize SPE conditions various sorbents were tested. Furthermore, high recoveries and good relative standard deviations were obtained when the samples were passed through the Oasis HLB column. The developed method was validated and successfully applied for determination of isoflavones in urine and plasma.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. D.F. Birt, S. Hendrich, W. Wang, Pharm. Ther. 90, 157 (2001)

    Article  CAS  Google Scholar 

  2. B.H. Havsteen, Pharmacol. Ther. 96, 67 (2002)

    Article  CAS  Google Scholar 

  3. T. Walle, Free Rad. Biol. Med. 36, 829 (2004)

    Article  CAS  Google Scholar 

  4. L.W. Lu, L.D. Broemeling, M.V. Marshall, V.S. Ramanujam, Cancer Epidemiol. Biomark. Prev. 4, 497 (1995)

    CAS  Google Scholar 

  5. K. Setchell, M. Faughnan, T. Avades, L. Zimmer-Nechemias, N. Brown, B. Wolfe, W. Brashear, P. Desai, M. Oldfield, N. Botting, A. Cassid, Am. J. Clin. Nutr. 77, 411 (2003)

    CAS  Google Scholar 

  6. A.A. Franke, L.J. Custer, Clin. Chem. 42, 955 (1996)

    CAS  Google Scholar 

  7. S. Yamamoto, T. Sobue, S. Sasaki, M. Kobayashi, Y. Arai, M. Uehara, H. Adlercreutz, S. Watanabe, T. Takahashi, Y. Iitoi, Y. Iwase, M. Akabane, S. Tsugane, J. Nutr. 131, 2741 (2001)

    CAS  Google Scholar 

  8. J. Maubach, M.E. Bracke, A. Heyerick, H.T. Depypere, R.F. Serreyn, M.M. Mareel, D. De Keukeleire, J. Chromatogr. B 784, 137 (2003)

    Article  CAS  Google Scholar 

  9. Z. Ma, Q. Wu, D.Y.W. Lee, M. Tracy, S.E. Lukas, J. Chromatogr. B 823, 108 (2005)

    Article  CAS  Google Scholar 

  10. B. Yan, D. Xing, Y. Ding, J. Tao, L. Du, J. Pharm. Biomed. Anal. 37, 297 (2005)

    Article  CAS  Google Scholar 

  11. S. Vega-López, K.J. Yeum, J.L. Lecker, L.M. Ausman, E.J. Johnson, S. Devaraj, I. Jialal, A.H. Lichtenstein, Am. J. Clin. Nutr. 81, 43 (2005)

    Google Scholar 

  12. C.L. Holder, M.I. Churchwell, D.R. Doerge, J. Agric. Food Chem. 47, 3764 (1999)

    Article  CAS  Google Scholar 

  13. N. Fang, S. Yu, T.M. Badger, J. Agric. Food Chem. 50, 2700 (2002)

    Article  CAS  Google Scholar 

  14. P.B. Grace, J.I. Taylor, N.P. Botting, T. Fryatt, M.F. Oldfield, S.A. Bingham, Anal. Biochem. 315, 114 (2003)

    Article  CAS  Google Scholar 

  15. P.B. Grace, J.I. Taylor, N.P. Botting, T. Fryatt, M.F. Oldfield, N. Al-Maharik, S.A. Bingham, Rapid Commun. Mass Spectrom. 17, 1350 (2003)

    Article  Google Scholar 

  16. P.B. Grace, N.S. Mistry, M.H. Carter, A.J.C. Leathem, P. Teale, J. Chromatogr. B 853, 138 (2007)

    Article  CAS  Google Scholar 

  17. L. Gu, M. Laly, H.C. Chang, R.L. Prior, N. Fang, M.J.J. Ronis, T.M. Badger, J. Agric. Food Chem. 53, 6858 (2005)

    Article  CAS  Google Scholar 

  18. J.K. Prasain, K. Jones, N. Brissie, R. Moore, J.M. Wyss, S. Barnes, J. Agric. Food Chem. 52, 3708 (2004)

    Article  CAS  Google Scholar 

  19. B. Klejdus, J. Vacek, V. Adam, J. Zehnálek, R. Kizek, L. Trnková, V. Kubáň, J. Chromatogr. B 806, 101 (2004)

    Article  CAS  Google Scholar 

  20. T. Nurmi, H. Adlercreutz, Anal. Biochem. 274, 110 (1999)

    Article  CAS  Google Scholar 

  21. J.A. Starkey, Y. Mechref, C.K. Byun, R. Steinmetz, J.S. Fuqua, O.H. Pescovitz, M.V. Novotny, Anal. Chem. 74, 5998 (2002)

    Article  CAS  Google Scholar 

  22. F. Kohen, S. Lichter, B. Gayer, J. De Boever, L.J.W. Lu, J. Steroid Biochem. Molec. Biol. 64, 217 (1998)

    Article  CAS  Google Scholar 

  23. M. Uehara, O. Lapcík, R. Hampl, N. Al-Maharik, T. Mäkelä, K. Wähälä, H. Mikola, H. Adlercreutz, J. Steroid. Biochem. Molecular. Biol. 72, 273 (2000)

    Article  CAS  Google Scholar 

  24. I. Baranowska, S. Magiera, Anal. Bioanal. Chem. 399, 3211 (2011)

    Article  CAS  Google Scholar 

  25. I. Baranowska, S. Magiera, J. Baranowski, J. Chromatogr. B 879, 615 (2011)

    Article  CAS  Google Scholar 

  26. A.R. Lomasney, C. Guillo, A.M. Sidebottom, M.G. Roper, Anal. Bioanal. Chem. 394, 313 (2009)

    Article  CAS  Google Scholar 

  27. E. Nemutlu, S. Kır, D. Katlan, M.S. Beksaç, Talanta 80, 117 (2009)

    Article  CAS  Google Scholar 

  28. A. Müller, D. Flottmann, W. Schulz, W. Seitz, W.H. Weber, Anal. Bioanal. Chem. 390, 1317 (2008)

    Article  Google Scholar 

  29. C. Hartmann, J. Smeyers-Verbeke, D.L. Massart, R.D. McDowall, J. Pharm. Biomed. Anal. 17, 193 (1998)

    Article  CAS  Google Scholar 

  30. H.F. Cueto-Rojas, N.O. Pérez, G. Pérez-Sánchez, I. Ocampo-Juárez, E. Medina-Rivero, J. Chromatogr. B 878, 1019 (2010)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Analytical Chemistry, Chemical Faculty, Silesian University of Technology, 44-100, Gliwice, Poland

    Irena Baranowska & Sylwia Magiera

  2. Department of Clinical Physiology, University Hospital, SE-581 85, Linköping, Sweden

    Jacek Baranowski

Authors
  1. Irena Baranowska
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sylwia Magiera
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jacek Baranowski
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Irena Baranowska.

About this article

Cite this article

Baranowska, I., Magiera, S. & Baranowski, J. Use of multivariate statistical techniques to optimize the separation of isoflavones by liquid chromatography. cent.eur.j.chem. 9, 972–981 (2011). https://doi.org/10.2478/s11532-011-0093-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2478/s11532-011-0093-8

Keywords

Search

Navigation