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Development of a rapid resolution HPLC method for the separation and determination of 17 phenolic compounds in crude plant extracts

  • Research Article
  • Published:
Central European Journal of Chemistry

Abstract

Rapid Resolution HPLC/DAD method, on a 1.8 µm, 4.6×50 mm column, was developed to enable a rapid separation of a mixture of 17 compounds, which consisted of hydroxybenzoic acids, hydroxycinnamic acids, flavones, flavonols, flavanone, flavonol-glycoside and antraquinone, in a single run, within 22 minutes. The developed method is precise, accurate and sensitive enough for simultaneous quantitative evaluation of major compounds in crude and hydrolyzed extracts of parsley, buckthorn, mint, caraway and birch. In order to overcome the inability to quantify all the phenolic compounds present in the samples caused by lack of external standards, HPLC approaches for the total phenolic content estimation based on sum of all integrated peak areas were made. These results were compared with the total phenolic content determined by Folin-Cioacalteu method. Although the correlation between the series of data was not significant (p<0.05), the difference between the results of total phenolic content obtained spectrophotometrically and by HPLC was not high in the case of parsley, buckthorn and mint extract. Regarding the obtained results, the HPLC approach could serve as an excellent tool for total phenolic content estimation, without the need for complete identification of the individual compounds.

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Authors and Affiliations

  1. Institute for Food Technology, University of Novi Sad, 21000, Novi Sad, Serbia

    Aleksandra Č. Mišan, Anamarija I. Mandić, Marijana B. Sakač, Ivan Lj. Milovanović & Ivana J. Sedej

  2. Faculty of Science, Department of Chemistry, University of Novi Sad, 21000, Novi Sad, Serbia

    Neda M. Mimica-Dukić

Authors
  1. Aleksandra Č. Mišan
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  2. Neda M. Mimica-Dukić
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  3. Anamarija I. Mandić
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  4. Marijana B. Sakač
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  5. Ivan Lj. Milovanović
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  6. Ivana J. Sedej
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Correspondence to Aleksandra Č. Mišan.

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Mišan, A.Č., Mimica-Dukić, N.M., Mandić, A.I. et al. Development of a rapid resolution HPLC method for the separation and determination of 17 phenolic compounds in crude plant extracts. cent.eur.j.chem. 9, 133–142 (2011). https://doi.org/10.2478/s11532-010-0126-8

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  • DOI: https://doi.org/10.2478/s11532-010-0126-8

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