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Interaction of serum albumin with vinyl sulfonate azo dye

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Central European Journal of Chemistry

Abstract

The interaction between bovine serum albumin and the mono azo reactive dye Orange ZT has been investigated using absorption difference spectroscopy. The influence of pH and ionic strength of the solution on the stability of the dye-protein complex has been determined. At 25°C, the complex dissociation constants were equal to 24.0, 28.0, 7.0, 11.0, 17.6 and 46.0 μM at pH 7.0, 6.5, 6.0, 5.5, 5.0 and 4.3, respectively. In the presence of 0.1, 0.2, 0.3 M KCl, at pH 6.0 and 25°C, the complex dissociation constants were 8.8, 20.0, 18.0 μM, respectively. The protein-dye complex dissociation constants show that Orange ZT could be used as an affinity ligand for protein purification.

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Authors and Affiliations

  1. Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, LT-10223, Vilnius-40, Lithuania

    Jolanta Sereikaite & Vladas-Algirdas Bumelis

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  1. Jolanta Sereikaite
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  2. Vladas-Algirdas Bumelis
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Correspondence to Jolanta Sereikaite.

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Sereikaite, J., Bumelis, VA. Interaction of serum albumin with vinyl sulfonate azo dye. cent.eur.j.chem. 6, 509–512 (2008). https://doi.org/10.2478/s11532-008-0066-8

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  • DOI: https://doi.org/10.2478/s11532-008-0066-8

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