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Simultaneous Determination of a New Dihydropyridine Calcium Blocker and Its Pyridine Metabolite in Dog Plasma by Column Switching High-Performance Liquid Chromatography with Electrochemical and Ultraviolet Detection

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Abstract

A highly sensitive, selective and rapid reversed-phase liquid chromatographic method using a column-switching technique has been developed for the simultaneous determination of a new dihydropyridine calcium blocker, methyl (±)-4,7-dihydrc-3-isobutyl-6-methyl-4-(3-nitrophenyl)thieno[2,3-b)]pyridine-5-carboxylate (I) and its pyridine metabolite, methyl 3-isobutyl-6-methyl-4-(3-nitrophenyl)thieno[2,3-b]pyridine-5-carboxylate (II), in dog plasma. Compounds I and II were determined with electrochemical detection at +0.50 V and by ultraviolet detection at 250 nm, respectively. The limits of the determination for compounds I and II were 0.2 and 2 ng ml”1, respectively, using 1 ml of dog plasma. Good liner relationships were found between the peak areas and the plasma concentrations of compounds I and II in the ranges from the lower limit of determination to 200 ng ml-1. The method was applied to preliminary pharmacokinetic studies after oral administration of compound I to beagle dogs.

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Authors and Affiliations

  1. Shionogi Research Laboratories, Shionogi & Co., Ltd., Sagisu, Fukushima, Osaka, 553, Japan

    Kyoji Ueno, Hiroyuki Fujitomo, Ikuko Nishino & Tsuneji Umeda

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  1. Kyoji Ueno
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  2. Hiroyuki Fujitomo
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  3. Ikuko Nishino
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  4. Tsuneji Umeda
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Ueno, K., Fujitomo, H., Nishino, I. et al. Simultaneous Determination of a New Dihydropyridine Calcium Blocker and Its Pyridine Metabolite in Dog Plasma by Column Switching High-Performance Liquid Chromatography with Electrochemical and Ultraviolet Detection. ANAL. SCI. 7, 727–731 (1991). https://doi.org/10.2116/analsci.7.727

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  • DOI: https://doi.org/10.2116/analsci.7.727

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