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Determination of Methamphetamine and Amphetamine in Urine by Headspace Gas Chromatography/Mass Spectrometry

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Abstract

The sensitivity of combined headspace gas chromatography (HSGC) and mass spectrometry (MS) for a stimulant (methamphetamine) and its urinary metabolite (amphetamine) was investigated. The analytical methods consisted of a combination of (1) HSGC and electron impact (EI)-MS, (2) HSGC, on-column trifluoroacetyl (TFA) derivatization of stimulants and EI-MS and (3) HSGC and chemical ionization (Cl)-MS using isobutane as a reagent gas. The combination of HSGC and EI-MS after on-line TFA-derivatization of stimulants (2) was the most informative method in these three procedure, but HSGC/CI-MS (3) was the most sensitive method. Detection limits of methamphetamine (MA) and amphetamine (AP) in urine by HSGC/CI-MS were found to be about 10 ng/ml (S/N>10) by monitoring quasimolecular ions of these compounds; m/z 150 and 136 observed on their mass spectra, respectively. Furthermore, the most sensitive CI-MS method was applied to quantivative analysis of these drugs excreted in urine by the selected ion monitoring (HSGC/CI-SIM).

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Authors and Affiliations

  1. Forensic Science Laboratory, Osaka Prefectural Police Head-Quarters, Otemae, Osaka, 540, Japan

    Hitoshi Tsuchihashi, Kunio Nakajima & Mayumi Nishikawa

  2. Identification Center, Kinki Regional Police Bureau, Otemae, Osaka, 540, Japan

    Shin-ichi Suzuki

  3. Analytical Application Laboratory, Shimadzu Corporation, Nishinokyo, Kuwabara-cho, Kyoto, 604, Japan

    Koichi Shiomi & Seiji Takahashi

Authors
  1. Hitoshi Tsuchihashi
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  2. Kunio Nakajima
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  3. Mayumi Nishikawa
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  4. Shin-ichi Suzuki
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  5. Koichi Shiomi
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  6. Seiji Takahashi
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Tsuchihashi, H., Nakajima, K., Nishikawa, M. et al. Determination of Methamphetamine and Amphetamine in Urine by Headspace Gas Chromatography/Mass Spectrometry. ANAL. SCI. 7, 19–22 (1991). https://doi.org/10.2116/analsci.7.19

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  • DOI: https://doi.org/10.2116/analsci.7.19

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