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Determination of the Degree of Branching of Amylopectin-Like Glucans Evaluated by Quinoxaline Formation with o-Phenylenediamine under Alkaline and Deoxygenated Conditions

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Abstract

Quinoxaline derivatives derived from glycogen and amylopectin-like glucans by treatment with acid or β-amylase, were used for evaluation of the degree of branching by an alkaline o-phenylenediamine (OPD) method. Ion-exchange resin column chromatography of quinoxalines derived from an amylopectin-β-limit dextrin showed that the glucan had an average chain length (C̅L̅) of 9.9 on the C-chain and 1.55 units of branched chains. A glycogen-β-limit dextrin had the C̅L̅ of 7.8 on the C-chain, with 2.38 units of branch. The degree of branching obtained from this alkaline OPD method agreed with those estimated by other conventional methods.

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Authors and Affiliations

  1. Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture, Mozu-Umemachi, Sakai, Osaka, 591, Japan

    Masanosuke Takagi, Yoshiyuki Daido, Masao Kuriyama & Naofumi Morita

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  1. Masanosuke Takagi
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  2. Yoshiyuki Daido
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  3. Masao Kuriyama
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  4. Naofumi Morita
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Takagi, M., Daido, Y., Kuriyama, M. et al. Determination of the Degree of Branching of Amylopectin-Like Glucans Evaluated by Quinoxaline Formation with o-Phenylenediamine under Alkaline and Deoxygenated Conditions. ANAL. SCI. 6, 529–534 (1990). https://doi.org/10.2116/analsci.6.529

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  • DOI: https://doi.org/10.2116/analsci.6.529

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