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On-Line Sample Enrichment and Cleanup for High Performance Liquid Chromatography with Column Switching Technique A Review

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Abstract

The usability of column-switching techniques of high performance liquid chromatography is reviewed with special interest given to the enrichment and cleanup of biological samples. In Section 2, band broadening and a fully automated operation system are especially noted. In Sections 3 and 4, it is emphasized that the recovery of on-line solid extraction in a precolumn is quantitative (100%), depending upon the combination of the stationary phase and the mobile phase. In Section 5, are elucidated the deproteinization and recovery from protein-bound entities by a size exclusion coupled with internal-surface reversed-phase techniques or by hydrophobic interaction. These methods are extensively applied to the biological samples, such as whole blood and tissue homogenates, which contain minute particles, by using a large-bore endfitting filter for the precolumn.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Fukuyama University, Higashimuracho, Fukuyama, 729-02, Japan

    Hideo Imai, Tsutomu Masujima & Gen Tamai

  2. Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Kasumi, Hiroshima, 734, Japan

    Ikue Morita-Wada

Authors
  1. Hideo Imai
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  2. Tsutomu Masujima
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  3. Ikue Morita-Wada
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  4. Gen Tamai
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Imai, H., Masujima, T., Morita-Wada, I. et al. On-Line Sample Enrichment and Cleanup for High Performance Liquid Chromatography with Column Switching Technique A Review. ANAL. SCI. 5, 389–393 (1989). https://doi.org/10.2116/analsci.5.389

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  • DOI: https://doi.org/10.2116/analsci.5.389

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