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On-Line Solid-Phase Extraction Capillary Electrophoresis Mass Spectrometry for Preconcentration and Clean-Up of Peptides and Proteins

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Capillary Electrophoresis of Proteins and Peptides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1466))

Abstract

One of the major drawbacks of capillary electrophoresis (CE) and other microscale separation techniques, for the analysis of low abundant peptides and proteins in complex samples, are the poor concentration limits of detection. Several strategies have been developed to improve CE sensitivity. Here, we describe an on-line solid-phase extraction capillary electrophoresis mass spectrometry method with a commercial C18 sorbent for clean-up and preconcentration of neuropeptides from highly diluted biological samples.

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Acknowledgement

This work was supported by grants from the Spanish Ministry of Economy and Competitiveness (CTQ2011-27130 and CTQ2014-56777-R).

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Authors and Affiliations

  1. Department of Analytical Chemistry, University of Barcelona, Av. Diagonal 647, Barcelona, 08028, Spain

    Fernando Benavente, Silvia Medina-Casanellas, Estela Giménez & Victoria Sanz-Nebot

Authors
  1. Fernando Benavente
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  2. Silvia Medina-Casanellas
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  3. Estela Giménez
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  4. Victoria Sanz-Nebot
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Corresponding author

Correspondence to Fernando Benavente .

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Editors and Affiliations

  1. Institut Galien Paris-Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Université Paris-Saclay, Châtenay-Malabry, France

    Nguyet Thuy Tran

  2. Institut Galien Paris-Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Université Paris-Saclay, Châtenay-Malabry, France

    Myriam Taverna

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Benavente, F., Medina-Casanellas, S., Giménez, E., Sanz-Nebot, V. (2016). On-Line Solid-Phase Extraction Capillary Electrophoresis Mass Spectrometry for Preconcentration and Clean-Up of Peptides and Proteins. In: Tran, N., Taverna, M. (eds) Capillary Electrophoresis of Proteins and Peptides. Methods in Molecular Biology, vol 1466. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-4014-1_6

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  • DOI: https://doi.org/10.1007/978-1-4939-4014-1_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-4012-7

  • Online ISBN: 978-1-4939-4014-1

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