Abstract
Ferrozine (FZ) preferentially stabilizes Fe(II) over Fe(III) to raise the ferric reduction potential and oxidize antioxidants. The advantages of the ferric-ferrozine method over other iron-based total antioxidant capacity assays were: (i) higher molar absorptivity and enhanced sensitivity, (ii) lower interference from foreign ions, (iii) wide pH tolerance (iv) additivity of the absorbances for mixtures. Solid-phase extraction (SPE) could be combined with spectrophotometry, because the magenta-colored anionic Fe(II)-FZ complex was quantitatively sorbed on Sephadex QAE A-25 resin. The sensitivity enhancement using the resin enabled us to conduct total antioxidant capacity (TAC) measurements of antioxidant-poor samples. The apparent molar absorptivity, linear concentration range and trolox equivalent antioxidant capacities (TEAC) of certain antioxidants were found. The calibration curves (lines) of trolox, rutin, and rosmarinic acid individually and in herbal infusions—by using the method of standard additions—were parallel, confirming that the added antioxidants did not interact with herbal constituents to cause chemical deviations from Beer’s law.
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References
R. L. Prior, X. Wu, and K. Schaich, J. Agric. Food Chem., 2005, 53, 4290.
R. Apak, K. Güçlü, B. Demirata, M. Özyürek, S. E. Çelik, B. Bektaşoğlu, K. I. Berker, and D. Özyurt, Molecules, 2007, 12, 1496.
M. G. Do Santo, C. V. Nunez, and H. D. Moya, Adv. Biol. Chem., 2013, 3, 525.
D. Huang, B. Ou, and R. L. Prior, J. Agric. Food Chem., 2005, 53, 1841.
M. Antolovich, P. D. Prenzler, E. Patsalides, S. Mcdonald, and K. Robards, Analyst, 2002, 127, 183.
M. Choupani and S. A. Delouee, Int. J. Adv. Biol. Biomed. Res., 2014, 2, 1340.
A. C. Payne, A. Mazzer, G. J. J. Clarkson, and G. Taylor, Food Sci. Nutr., 2013, 1, 439.
N. Pellegrini, M. Serafini, B. Colombi, D. Del Rio, S. Salvatore, M. Bianchi, and F. Brighenti, J. Nutr., 2003, 133, 2812.
K. I. Berker, K. Güçlü, İ. Tor, and R. Apak, Talanta, 2007, 72, 1157.
R. Apak, M. Özyürek, K. Güçlü, and E. Çapanoğlu, J. Agric. Food. Chem., 2016, 64, 997.
M. Oyaizu, Jpn. J. Nutr., 1986, 44, 307.
I. F. F. Benzie and J. J. Strain, Anal. Biochem., 1996, 239, 70.
L. L. Stookey, Anal. Chem., 1970, 42, 779.
J. C. Thompsen and H. A. Mottola, Anal. Chem., 1984, 56, 755.
E. Viollier, P. W. Inglett, K. Hunter, A. N. Roychoudhury, and P. Van Cappellen, Appl. Geochem., 2000, 15, 785.
D. L. Giokas, E. K. Paleologos, and M. I. Karayannis, Anal. Bioanal. Chem., 2002, 373, 237.
A. Molina-Diaz, I. Ortega-Carmona, and M. I. Pascual-Reguera, Talanta, 1998, 4, 531.
I. Gulcin, M. Oktay, E. Kirecci, and O. I. Kufrevioglu, Food Chem., 2003, 83, 371.
I. Gulcin, Amino Acids, 2007, 32, 431.
K. I. Berker, K. Guclu, B. Demirata, and R. Apak, Anal. Methods, 2010, 2, 1770.
P. O. Barrales, M. L. Fernândez de Cordova, and A. M. Diaz, Anal. Chim. Acta, 1998, 360, 143.
M. L. Fernández de Cordova, A. Ruqâz-Medina, and A. M. Diaz, J. Anal. Chem., 1997, 357, 44.
K. Yoshimura, H. Waki, and S. Ohashi, Talanta, 1976, 23, 449.
F. F. Naieni, B. Ebrahimi, H. R. Vakilian, and Z. Shahmoradi, Biol. Trace Elem. Res., 2012, 146, 30.
K. Mehta, Y.-H. Tu, and M. Shah, “Formulations Containing an Ionic Mineral-ion Exchange Resin Complex and Uses Thereof”, US Patent No: 8,287,848 B2, Oct. 16, 2012.
S. Karakaya, S. N. El, and A. A. Tas, Int. J. Food Sci. Nutr., 2001, 52, 501.
Acknowledgments
One of the authors (K. I. B.) is thankful to TUBITAK Research Fund for their support to the Project 112T 793. Another one of the authors (R. A.) extends his gratitude to the Centre for Measurement and Application of Food Antioxidants of Istanbul University (I. U. Gida Antioksidanlari Olcumu Uygulama ve Arastirma Merkezi) for the general support given to the development of analytical problem-focused antioxidant activity assays.
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Berker, K.I., Ozyurt, D., Demirata, B. et al. Solid-Phase Extraction Spectrophotometric Determination of Total Antioxidant Capacity in Antioxidant-poor Samples by Using the Ferric-Ferrozine Method. ANAL. SCI. 33, 683–689 (2017). https://doi.org/10.2116/analsci.33.683
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DOI: https://doi.org/10.2116/analsci.33.683