Abstract
Fluorescence derivatization of the oligosaccharides released from glycoconjugates is widely used for precise structural characterization. To ensure labeling of the oligosaccharides, a large excess of fluorescence reagents is usually added to the reaction tube. Therefore, any excess reagents and by-products of the labeling reaction should be removed by several column chromatographies, including using a cellulose cartridge or spin columns. However, these purification steps are often time-consuming, expensive, and laborious. In this study, we found that 1,2-dichloroethane extraction could effectively and easily purify pyridylaminated oligosaccharides with a high recovery rate.
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Acknowledgments
This work was supported by Nihon University College of Science and Technology Grant in Aid for Fundamental Science Research (Y. S.), and partly supported by Grant-in-Aid for Young Scientists (B) No. 24750164 from MEXT Japan (Y. S.). The authors declare no conflicts of interest.
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Suzuki, Y., Okano, A., Kabayama, K. et al. Purification of Pyridylaminated Oligosaccharides Using 1,2-Dichloroethane Extraction. ANAL. SCI. 32, 487–490 (2016). https://doi.org/10.2116/analsci.32.487
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DOI: https://doi.org/10.2116/analsci.32.487