Abstract
There is an urgent need to develop novel in-vitro models to mimic the disease conditions in pulmonary hypertension (PH). We developed a microfluidic cell culture device for PH studies that withstood high shear stress. Techniques were also developed for cell recovery from the microchannel and mRNA isolation from the collected cells. Using this device, we found that shear stress caused a 7.5-fold increase in the transcription levels of a PH-related molecule, Cyclin DI.
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Acknowledgments
This study was supported in part, by Banyu Foundation Research Grant. The Science Research Promotion Fund. Suzuken Memorial Foundation. Shiseido Female Researcher Science Grant. Koyanagi Foundation Research Grant, and a Grant-in-Aid for Scientific Research (JSPS. KAKENHI; Grant Numbers 25600065 to K. S„ 16HO417O to K. S.. and 16K09488 toA. O.).
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Sato, K., Nakajima, M., Tokuda, S. et al. Fluidic Culture and Analysis of Pulmonary Artery Smooth Muscle Cells for the Study of Pulmonary Hypertension. ANAL. SCI. 32, 1217–1221 (2016). https://doi.org/10.2116/analsci.32.1217
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DOI: https://doi.org/10.2116/analsci.32.1217