Skip to main content
SpringerLink
Log in

On-chip FRET Graphene Oxide Aptasensor: Quantitative Evaluation of Enhanced Sensitivity by Aptamer with a Double-stranded DNA Spacer

  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

We propose a molecular design for a biomolecular probe to realize an on-chip graphene oxide (GO) aptasensor with enhanced sensitivity. Here, GO works as an excellent acceptor for fluorescence resonance energy transfer. We inserted a rigid double-stranded DNA as a spacer between the GO surface and the aptamer sequence to extend the distance between a fluorescence dye and the GO surface during molecular recognition. We examined the dependence of the sensitivity on the length of the spacer quantitatively by using a 2×2 linear-array aptasensor. We used the modified aptamer with 10 and 30 base pair (bp) double-stranded DNA spacers. The signal with a 30bp-spacer was about twice as strong that with a 10bp-spacer as regards both thrombin and prostate specific antigen detections. The improvement in the sensitivity was supported by a model calculation that estimated the effect of spacer length on fluorescence recovery efficiency.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. C. N. R. Rao and A. K. Sood, “Grapheme: Synthesis, Properties, and Phenomena”, 1st ed., 2013, Wiley-VCH, Weinheim.

    Google Scholar 

  2. D. Li, M. B. Müller, S. Gilje, R. B. Kaner, and G. G. Wallace, Nat. Nanotechnol., 2008, 3, 101.

    Article  CAS  PubMed  Google Scholar 

  3. S. Park and R. S. Ruoff, Nat. Nanotechnol., 2009, 4, 217.

    Article  CAS  PubMed  Google Scholar 

  4. K. P. Loh, Q. Bao, G. Eda, and M. Chhowalla, Nat. Chem., 2010, 2, 1015.

    Article  CAS  PubMed  Google Scholar 

  5. K. Erickson, R. Erni, Z. Lee, N. Alem, W. Gannett, and A. Zettl, Adv. Mater., 2010, 22, 4467.

    Article  CAS  PubMed  Google Scholar 

  6. E. Treossi, M. Melucci, A. Liscio, M. Gazzano, P. Samorf, and V. Palermo, J. Am. Chem. Soc., 2009, 737, 15576.

    Article  Google Scholar 

  7. Y. Liu, C.-Y. Liu, and Y. Liu, Appl. Surf. Sci., 2011, 257, 5513.

    Article  CAS  Google Scholar 

  8. S. Husale, S. Sahoo, A. Radenovic, F. Traversi, P. Annibale, and A. Kis, Langmuir, 2010, 26, 18078.

    Article  CAS  PubMed  Google Scholar 

  9. M. Wu, R. Kempaiah, P.-J. J. Huang, V. Maheshwari, and J. Liu, Langmuir, 2011, 27, 2731.

    Article  CAS  PubMed  Google Scholar 

  10. S. Klussmann, “The Aptamer Handbook: Functional Oligonucleotides and Their Applications”, 1st ed., 2006, Wiley-VCH, Weinheim.

    Book  Google Scholar 

  11. E. Morales-Narváez and A. Merkoçi, Adv. Mater., 2012, 24, 3298, and references therein.

    Article  PubMed  Google Scholar 

  12. L. Gao, C. Lian, Y. Zhou, L. Yan, Q. Li, C. Zhang, L. Chen, and K. Chen, Biosens. Bioelectron., 2014, 60, 22, and references therein.

    Article  CAS  PubMed  Google Scholar 

  13. Z. Liu, B. Liu, J. Ding, and J. Liu, Anal. Bioanal. Chem., 2015, 406, 6885, and references therein.

    Article  Google Scholar 

  14. K. Furukawa, Y. Ueno, E. Tamechika, and H. Hibino, J. Mater. Chem. B, 2013, 7, 1119.

    Article  Google Scholar 

  15. R. J. Chen, Y. Zhang, D. Wang, and H. Dai, J. Am. Chem. Soc., 2001, 723, 3838.

    Article  Google Scholar 

  16. Y. Ohno, K. Maehashi, and K. Matsumoto, J. Am. Chem. Soc., 2010, 732, 18012.

    Article  Google Scholar 

  17. H. Chang, L. Tang, Y. Wang, J. Jiang, and J. Li, Anal. Chem., 2010, 82, 2341.

    Article  CAS  PubMed  Google Scholar 

  18. L. Cao, L. Cheng, Z. Zhang, Y. Wang, X. Zhang, H. Chen, B. Liu, S. Zhang, and J. Kong, Lab Chip, 2012, 72, 4864.

    Article  Google Scholar 

  19. Y. Ueno, K. Furukawa, K. Matsuo, S. Inoue, K. Hayashi, and H. Hibino, Anal. Chim. Acta, 2015, 866, 1.

    Article  CAS  PubMed  Google Scholar 

  20. R. Nutiu and Y. Li, J. Am. Chem. Soc., 2003, 725, 4771.

    Article  Google Scholar 

  21. L.-P. Qiu, Z.-S. Wu, G.-L. Shen, and R.-Q. Yu, Anal. Chem., 2011, 83, 3050.

    Article  CAS  PubMed  Google Scholar 

  22. S. Nakayama, Y. Luo, J. Zhou, T. K. Dayie, and H. O. Sintim, Chem. Commun., 2012, 48, 9059.

    Article  CAS  Google Scholar 

  23. Y. Ueno, K. Furukawa, K. Matsuo, S. Inoue, K. Hayashi, and H. Hibino, Chem. Commun., 2013, 49, 10346.

    Article  CAS  Google Scholar 

  24. F. Liu, J. Y. Choi, and T. S. Seo, Biosens. Bioelectron., 2010, 25, 2361.

    Article  CAS  PubMed  Google Scholar 

  25. F. Li, Y. Huang, Q. Yang, Z. Zhoung, D. Li, L. Wang, S. Song, and C. Fan, Nanoscale, 2010, 2, 1021.

    Article  CAS  PubMed  Google Scholar 

  26. H. Jang, Y.-K. Kim, H.-M. Kwon, W.-S. Yeo, D.-E. Kim, and D.-H. Min, Angew. Chem., Int. Ed., 2010, 49, 5703.

    Article  CAS  Google Scholar 

  27. L. C. Bock, L. C. Griffin, L. A. Latham, E. H. Vermaas, and J. J. Toole, Nature, 1992, 355, 564.

    Article  CAS  PubMed  Google Scholar 

  28. N. Savory, K. Abe, K. Sode, and K. Ikebukuro, Biosens. Bioelectron., 2010, 26, 1386.

    Article  CAS  PubMed  Google Scholar 

  29. P. J. J. Huang and J. Liu, Small, 2012, 8, 977.

    Article  CAS  PubMed  Google Scholar 

  30. B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, and P. Walter, “Molecular Biology of the Cell”, 5th ed., 2007, Garland Science, New York.

    Book  Google Scholar 

  31. S. Rinker, Y. Ke, Y. Liu, R. Chhabra, and H. Yan, Nat. Nanotechnol., 2008, 3, 418.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. NTT Basic Research Laboratories, NTT Corp., 3-1 Morinosato Wakamiya, Atsugi, Kanagawa, 243-0198, Japan

    Yuko Ueno, Kazuaki Furukawa, Andrew Tin & Hiroki Hibino

Authors
  1. Yuko Ueno
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kazuaki Furukawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Andrew Tin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hiroki Hibino
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yuko Ueno.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ueno, Y., Furukawa, K., Tin, A. et al. On-chip FRET Graphene Oxide Aptasensor: Quantitative Evaluation of Enhanced Sensitivity by Aptamer with a Double-stranded DNA Spacer. ANAL. SCI. 31, 875–879 (2015). https://doi.org/10.2116/analsci.31.875

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.31.875

Keywords

Search

Navigation