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Sequence-selective Modification of DNA Cytosine by Using Junction-forming DNA Probes and Its Application to the Detection of Single Cytosine Methylation

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Abstract

Methylated DNA plays an important role in epigenetic gene regulation, and could therefore serve as a potentially promising biomarker for the diagnosis of cancer and other diseases. Therefore, the development of a simple method for analyzing cytosine methylation in a target gene is required. Here, we report on a new method for the sequence-selective chemical modification of a single cytosine in single-stranded DNA (ssDNA). This method is based on the formation of a DNA three-way junction of the ssDNA and two ssDNA probes, and was successfully applied for a simple polymerase chain reaction (PCR)-based assay for ‘pinpoint’ methylation analysis.

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Authors and Affiliations

  1. Graduate School of Bionics, Computer and Media Sciences, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo, 192-0982, Japan

    Kenta Takanashi & Teru Kato

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  1. Kenta Takanashi
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  2. Teru Kato
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Correspondence to Teru Kato.

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Takanashi, K., Kato, T. Sequence-selective Modification of DNA Cytosine by Using Junction-forming DNA Probes and Its Application to the Detection of Single Cytosine Methylation. ANAL. SCI. 30, 371–376 (2014). https://doi.org/10.2116/analsci.30.371

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  • DOI: https://doi.org/10.2116/analsci.30.371

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