Skip to main content
SpringerLink
Log in

Optimization of an Online Post-Column Derivatization System for Ultra High-Performance Liquid Chromatography (UHPLC) and Its Applications to Analysis of Biogenic Amines

  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

We investigated the efficacy and utility of a post-column derivatization method for the detection of amines with o-phthalaldehyde (OPA) in ultra high-performance liquid chromatography (UHPLC). Since it was anticipated that a derivatization reaction system placed downstream of the column would give considerable band broadening to narrow peaks generated by a UHPLC column, we examined the contributions of the dimensions of the reaction tube, the fluorescence (FL) detector flow cell, and the column to the extra-column peak broadening, and optimized the post-column derivatization system in UHPLC. We successfully separated amines within 6 min with gradient elution by using the post-column derivatization system connected to a UHPLC system. The analysis time was reduced by a factor of 7 compared with that by conventional HPLC. The detection limits were 18.0 - 105.7 fmol, depending on the compounds; the reproducibilities were 0.11 – 0.82% RSD for the retention times and 0.78 – 1.66% RSD for the peak areas (n = 10), respectively. The linear dynamic ranges were up to 12, 20, and 40 pmol/μL, depending on the compounds, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. I. S. Krull, Z. Deyl, and H. Lingeman, J. Chromatogr., B, 1994, 659, 1.

    Article  CAS  Google Scholar 

  2. S. Hernández-Cassou and J. Saurina, J. Chromatogr., B, 2011, 879, 1270.

    Article  Google Scholar 

  3. R. S. Deelder, M. G. F. Kroll, and J. H. M. Van den Berg, J. Chromatogr., A, 1976, 125, 307.

    Article  CAS  Google Scholar 

  4. R. S. Deelder, M. G. F. Kroll, A. J. B. Beeren, and J. H. M. Van den Berg, J. Chromatogr., A, 1978, 149, 669.

    Article  CAS  Google Scholar 

  5. R. S. Deelder, A. T. J. M. Kuijpers, and J. H. M. Van den Berg, J. Chromatogr., A, 1983, 255, 545.

    Article  CAS  Google Scholar 

  6. M. Yoshioka, Z. Tamura, M. Senda, and T. Miyazaki, J. Chromatogr., B, 1985, 344, 345.

    Article  CAS  Google Scholar 

  7. T. Takeuchi, S. Saito, and D. Ishii, J. Chromatogr., A, 1983, 258, 125.

    Article  CAS  Google Scholar 

  8. M. Zhang, T. Caragine, H. Wang, P. S. Cohen, G. Botchkina, K. Soda, M. Bianchi, P. Ulroch, A. Cerami, B. Sherry, and K. J. Tracey, J. Exp. Med., 1997, 185, 1759.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. K. Soda, Y. Kano, T. Nakamura, K. Kasono, M. Kawakami, and F. Konishi, J. Immunol., 2005, 175, 237.

    Article  CAS  PubMed  Google Scholar 

  10. H. M. H. van Eijk, D. R. Rooyakkers, and N. E. P. Deutz, J. Chromatogr., A, 1996, 730, 115.

    Article  PubMed  Google Scholar 

  11. M. Venza, M. Visalli, D. Cicciu, and D. Teti, J. Chromatogr., B, 2001, 757, 111.

    Article  CAS  Google Scholar 

  12. K. B. Male and J. H. T. Luong, J. Chromatogr., A, 2001, 926, 309.

    Article  CAS  PubMed  Google Scholar 

  13. A. Zotou, Z. Loukou, E. Soufleros, and I. Stratis, Chromatographia, 2003, 57, 429.

    Article  CAS  Google Scholar 

  14. Z. Loukou and A. Zotou, J. Chromatogr., A, 2003, 996, 103.

    Article  CAS  PubMed  Google Scholar 

  15. V. Lozanov, S. Petrov, and V. Mitev, J. Chromatogr., A, 2004, 1025, 201.

    Article  CAS  PubMed  Google Scholar 

  16. M. C. Vidal-Carou, F. Lahoz-Portolés, S. Bover-Cid, and A. Mariné-Font, J. Chromatogr., A, 2003, 998, 235.

    Article  CAS  PubMed  Google Scholar 

  17. T. Lavizzari, M. T. Veciana-Nogués, S. Bover-Cid, A. Mariné-Font, and M. C. Vidal-Carou, J. Chromatogr., A, 2006, 1129, 67.

    Article  CAS  PubMed  Google Scholar 

  18. M. L. Latorre-Moratalla, J. Bosch-Fusté, T. Lavizzari, S. Bover-Cid, M. T. Veciana-Nogués, and M. C. Vidal-Carou, J. Chromatogr., A, 2009, 1216, 7715.

    Article  CAS  PubMed  Google Scholar 

  19. S. Moret and L. S. Conte, J. Chromatogr., A, 1996, 729, 363.

    Article  CAS  PubMed  Google Scholar 

  20. T. Sakamoto, K. Akaki, and H. Hiwaki, Food Hyg. Saf. Sci., (in Japanese with English summary) 2010, 51, 115.

    Article  CAS  Google Scholar 

  21. C. Buteau, C. L. Duitschaever, and G. C. Ashton, Am. J. Enol. Vitic., 1984, 35, 228.

    Article  CAS  Google Scholar 

  22. C. S. Ough and C. E. Daudt, Am. J. Enol. Vitic., 1981, 32, 185.

    Article  CAS  Google Scholar 

  23. P. M. Izquierdo Cañas, E. Garcia Romero, S. Gómez Alonso, M. Fernández González, and M. L. L. Palop Herreros, J. Food Comp. Anal., 2008, 21, 731.

    Article  Google Scholar 

  24. M. C. Vidal-Carou, A. Ambatlle-Espunyes, M. C. Ulla- Ulla, and A. Mariné-Font, Am. J. Enol. Vitic., 1990, 41, 160.

    Article  CAS  Google Scholar 

  25. C. Ancín-Azpilicueta, A. González-Marco, and N. Jiménez-Moreno, Crit. Rev. Food Sci. Nutr., 2008, 48, 257.

    Article  PubMed  Google Scholar 

  26. A. P. Marques, M. C. Leitáo, and M. V. San Romão, Food Chem., 2008, 107, 853.

    Article  CAS  Google Scholar 

  27. F. Cecchini and M. Morassut, Food Chem., 2010, 123, 263.

    Article  CAS  Google Scholar 

  28. L. Manfroi, P. H. A. Silva, L. A. Rizzon, P. S. Sabaini, and M. B. A. Glória, Food Chem., 2009, 116, 208.

    Article  CAS  Google Scholar 

  29. I. Rosi, F. Nannelli, and G. Giovani, LWT–Food Sci. Technol., 2009, 42, 525.

    Article  CAS  Google Scholar 

  30. A. Ibe, Ann. Rep. Tokyo Metr. Inst. P.H. (in Japanese), 2004, 55, 13.

    CAS  Google Scholar 

  31. M. Nakazato, C. Kobayashi, Y. Yamajima, Y. Tateishi, Y. Kawai, and K. Yasuda, Ann. Rep. Tokyo Metr. Res. Lab. P.H. (in Japanese), 2002, 53, 95.

    Google Scholar 

  32. R. Stute, K. Petridis, H. Steinhart, and G. Biernoth, Eur. Food Res. Technol., 2002, 215, 101.

    Article  CAS  Google Scholar 

  33. N. G. Sanceda, E. Suzuki, M. Ohashi, and T. Kurata, J. Agric. Food Chem., 1999, 47, 3596.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. LC and SFC Applications Laboratory, JASCO Corporation, 2967-5, Tokyo 192-8537, Ishikawamachi, Hachioji, Japan

    Satoe Iijima, Yasuyo Sato, Masao Bounoshita, Toshihiko Miyaji & Muneo Saito

  2. JASCO Incorporated, 28600 Mary’s Court, 21601, Easton, MD, USA

    David J. Tognarelli

Authors
  1. Satoe Iijima
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yasuyo Sato
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Masao Bounoshita
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Toshihiko Miyaji
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. David J. Tognarelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Muneo Saito
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Satoe Iijima.

Additional information

This article was presented in part as poster No. P-475-Wed at HPLC 2012, June 16 – 21 held in Anaheim, CA, USA.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Iijima, S., Sato, Y., Bounoshita, M. et al. Optimization of an Online Post-Column Derivatization System for Ultra High-Performance Liquid Chromatography (UHPLC) and Its Applications to Analysis of Biogenic Amines. ANAL. SCI. 29, 539–545 (2013). https://doi.org/10.2116/analsci.29.539

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.29.539

Search

Navigation