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Stable Lipid Bilayers Based on Micro- and Nano-Fabrication as a Platform for Recording Ion-Channel Activities

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Abstract

In this review, we will discuss our recent approaches for the formation of mechanically stable bilayer lipid membranes (BLMs) by combining with BLM formation and micro- and nano-fabrication techniques. BLMs were prepared across a microaperture fabricated in silicon (Si) chips or nanoporous alumina films using a minimized amount of organic solvent. Although BLMs spanned over the porous alumina film showed better electrical properties, such as background current noise and current transient, BLMs suspended in a thin Si3N4 septum showed a much superior BLM stability. The BLMs showed tolerance to a high voltage of ±1 V, a membrane lifetime of >40 h, and tolerance to repetitive solution exchanges. Application to a drug screening system has been examined by using the human ether-a-go-go-related gene (hERG) potassium channel as an illustrative example. The potentiality of the present system as a platform of the high-throughput analysis for ion-channel protein is also discussed.

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Authors and Affiliations

  1. Graduate School of Biomedical Engineering, Tohoku University, 6-6 Aoba, 980-8579, Aramaki, Aoba, Sendai, Miyagi, Japan

    Ayumi Hirano-Iwata, Azusa Oshima, Hideki Mozumi & Michio Niwano

  2. PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho, 332-0012, Kawaguchi, Saitama, Japan

    Ayumi Hirano-Iwata

  3. Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University, 2-1-1 Katahira, 980-8577, Sendai, Miyagi, Japan

    Yasuo Kimura & Michio Niwano

Authors
  1. Ayumi Hirano-Iwata
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  2. Azusa Oshima
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  3. Hideki Mozumi
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  4. Yasuo Kimura
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  5. Michio Niwano
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Correspondence to Ayumi Hirano-Iwata.

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Hirano-Iwata, A., Oshima, A., Mozumi, H. et al. Stable Lipid Bilayers Based on Micro- and Nano-Fabrication as a Platform for Recording Ion-Channel Activities. ANAL. SCI. 28, 1049–1057 (2012). https://doi.org/10.2116/analsci.28.1049

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  • DOI: https://doi.org/10.2116/analsci.28.1049

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