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Detection of an Antibody to Avian Influenza Virus by an Electrochemical Immunoassay (eELISA)

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Abstract

The detection of an antibody against nucleoprotein (NP) of the avian influenza virus (AIV) was attempted by an electrochemical immunoassay by combining a secondary antibody-alkaline phosphatase conjugate and 4-aminophenylphosphate. Released 4-aminophenol was quantitated electrochemically, and the current derived from oxidation of the hydrolysis product increased linearly over a wide primary antibody concentration range (10–1000 ng/ml). The detection limit of this electrochemical immunoassay for the antibody against the NP of AIV was found to be 10 ng/ml.

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References

  1. R. A. M. Fouchier, V. Munster, A. Wallensten, T. M. Bestebroer, S. Herfst, D. Smith, G. F. Rimmelzwaan, B. Olsen, and A. D. M. E. Osterhaus, J. Virol, 2005, 79, 2814.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. K. G. Nicholson, J. M. Wood, and M. Zambon, Lancet, 2003, 362, 1733.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. E. Carraro, D. F. Neto, D. Benfica, A. H. SittaPerosa, C. F. H. Granato, and N. C. J. Bellei, Diagn. Micr. Infec. Dis., 2007, 57, 53.

    Article  CAS  Google Scholar 

  4. D. E. Swayne and D. A. Halvorson, in “Diseases of Poultry”, ed. Y. M. Saif, 11th ed., 2003, Iowa State Press, Ames, 135.

  5. R. A. M. Fouchier, T. M. Bestebroer, S. Herfst, L. V. D. Kemp, G. F. Rimmelzwaan, and A. D. M. E. Osterhaus, J. Clin. Microbiol, 2000, 38, 4096.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. B. Chaharaein, A. R. Omar, I. Aini, K. Yusoff, and S. S. Hassan, Arch. Virol, 2006, 151, 2447.

    Article  CAS  PubMed  Google Scholar 

  7. F. R. Brishai and R. Galli, J. Clin. Microbiol, 1978, 8, 648.

    Article  Google Scholar 

  8. A. L. Shafer, J. B. Katz, and K. A. Eernisse, Avian Dis., 1998, 42, 28.

    Article  CAS  PubMed  Google Scholar 

  9. E.-M. Zhou, M. Chan, R. A. Heckert, J. Riva, and M.-F. Cantin, Avian Dis., 1998, 42, 517.

    Article  CAS  PubMed  Google Scholar 

  10. E. Starick, O. Werner, H. Schirrmeier, B. Kollner, R. Riebe, and E. Mundt, J. Vet. Med. B Infect. Dis. Vet. Public Health, 2006, 53, 370.

    Article  CAS  PubMed  Google Scholar 

  11. R. Wu, S. Hu, Y. Xiao, Z. Li, D. Shi, and D. Bi, Vet. Res. Commun., 2007, 31, 631.

    Article  CAS  PubMed  Google Scholar 

  12. M. Yang, Y. Berhane, T. Salo, M. Li, K. Hole, and A. Clavijo, J. Virol. Methods, 2008, 147, 265.

    Article  CAS  PubMed  Google Scholar 

  13. P. Skladal, Electro analysis, 1997, 9, 737.

    Article  CAS  Google Scholar 

  14. A. Warsinke, A. Benkert, and F. W. Scheller, Fresenius J. Anal. Chem., 2000, 366, 622.

    Article  CAS  PubMed  Google Scholar 

  15. L. Nyholm, Analyst, 2005 130, 599.

    Article  CAS  PubMed  Google Scholar 

  16. E. P. Gil, H. T. Tang, H. B. Halsall, W. R. Heineman, and A. S. Misiego, Clin. Chem., 1990, 36, 662.

    Article  CAS  PubMed  Google Scholar 

  17. O. Niwa, Y. Xu, H. B. Halsall, and W. R. Heineman, Anal. Chem., 1993, 65, 1559.

    Article  CAS  PubMed  Google Scholar 

  18. J. E. Frew, N. C. Foulds, M. Wilshere, N. J. Forrow, and M. J. Green, J. Electroanal. Chem., 1989, 266, 309.

    Article  CAS  Google Scholar 

  19. K. Inoue, P. Ferrante, Y Hirano, T. Yasukawa, H. Shiku, and T. Matsue, Talanta, 2007, 73, 886.

    Article  CAS  PubMed  Google Scholar 

  20. D. Ogasawara, Y Hirano, T. Yasukawa, H. Shiku, K. Kobori, K. Ushizawa, S. Kawabata, and T. Matsue, Biosens. Bioelectron., 2006, 21, 1784.

    Article  CAS  PubMed  Google Scholar 

  21. F M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl, “Current Protocols in Molecular Biology”, 1987, Vol. 2, Green Publishing Associates, Inc., and Joh. Wile. & Sons, Inc., Hoboken, NJ.

    Google Scholar 

  22. J. Lahiri, L. Isaacs, J. Tien, and G. M. Whitesides, Anal. Chem., 1999, 71, 111.

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Authors and Affiliations

  1. Department of Applied Chemistry, Kyushu Institute of Technology, 1-1 Sensui, Tobata, Kitakyushu, 804-8550, Japan

    Keiichi Ohtsuka & Shigeori Takenaka

  2. Hano Manufacturing Co. Ltd., 1-1-12 Hakomatsu, Higashi, Fukuoka, 812-0061, Japan

    Hiroshi Endo & Kimio Morimoto

  3. Research Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, 080-8555, Japan

    Bui N. Vuong, Haruko Ogawa & Kunitoshi Imai

Authors
  1. Keiichi Ohtsuka
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  2. Hiroshi Endo
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  3. Kimio Morimoto
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  4. Bui N. Vuong
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  5. Haruko Ogawa
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  6. Kunitoshi Imai
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  7. Shigeori Takenaka
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Correspondence to Shigeori Takenaka.

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Ohtsuka, K., Endo, H., Morimoto, K. et al. Detection of an Antibody to Avian Influenza Virus by an Electrochemical Immunoassay (eELISA). ANAL. SCI. 24, 1619–1622 (2008). https://doi.org/10.2116/analsci.24.1619

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  • DOI: https://doi.org/10.2116/analsci.24.1619

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