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Zone Electrophoresis in an Inner-cooling Wide-bore Electrophoresis System with UV Detection

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Abstract

A novel, high-performance wide-bore electrophoresis (WE) system with inner-cooling has been developed. By introducing the mode of a shell and tube heat exchanger into this system to remove Joule heat generated during electrophoresis, it is feasible to extend electrophoresis from the conventional capillary (i.d. <100 µm) to a wide-bore tube (i.d. >1000 µm). The wide tube allows the loading of over 1.0 µL of the sample with an LOD of 3.0 × 10-4 mg/mL (signal-to-noise ratio, 3:1). Satisfactory separations of model compounds have been achieved on the WE system.

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References

  1. A. R. Timerbaev, Electrophoresis, 2002, 23, 3884.

    Article  CAS  Google Scholar 

  2. A. Cifuentes, Electrophoresis, 2006, 27, 283.

    Article  CAS  Google Scholar 

  3. J. W. Cooper, Y. Wang, and C. S. Lee, Electrophoresis, 2004, 25, 3913.

    Article  CAS  Google Scholar 

  4. D. Perrett, Ann. Clin. Biochem., 1999, 36, 133.

    Article  CAS  Google Scholar 

  5. K. W. Ro, K. Lim, B. C. Shim, and J. H. Hahn, Anal. Chem., 2005, 77, 5160.

    Article  CAS  Google Scholar 

  6. S. N. Bendrysheva, M. A. Proskurnin, U. Pyell, and W. Faubel, Anal. Bioanal. Chem., 2006, 385, 1492.

    Article  CAS  Google Scholar 

  7. S. P. Porras, M. Jussila, K. Sinervo, and M. L. Riekkola, Electrophoresis, 1999, 20, 2510.

    Article  CAS  Google Scholar 

  8. I. E. Valko, S. P. Porras, and M. L. Riekkola, J. Chromatogr., A, 1998, 813, 179.

    Article  CAS  Google Scholar 

  9. M. L. Riekkola, M. Jussila, S. P. Porras, and I. E. Valko, J. Chromatogr., A, 2000, 892, 155.

    Article  CAS  Google Scholar 

  10. H. F. Yin, C. Keely-Templin, and D. J. McManigill, J. Chromatogr., A, 1996, 744, 45.

    Article  CAS  Google Scholar 

  11. J. R. Chen, R. N. Zare, E. C. Peters, F. Svec, and J. J. Frechet, Anal. Chem., 2001, 73, 1987.

    Article  CAS  Google Scholar 

  12. Q. S. Qu, Y. Z. He, W. E. Gan, N. Deng, and X. Q. Lin, J. Chromatogr., A, 2003, 983, 255.

    Article  CAS  Google Scholar 

  13. Q. S. Qu, R. J. Qu, Q. Xu, Y. B. Wang, X. R. Wang, and Y. Z. He, J. Sep. Sci., 2004, 27, 725.

    Article  CAS  Google Scholar 

  14. Y. G. Guo, D. N. Liu, H. F. Wang, R. J. Yuan, and J. J. Bao, Chem. Lett., 2006, 35, 1386.

    Article  CAS  Google Scholar 

  15. M. A. Bosse and P. Arce, Electrophoresis, 2000, 21, 1018.

    Article  CAS  Google Scholar 

  16. M. A. Bosse and P. Arce, Electrophoresis, 2000, 21, 1026.

    Article  CAS  Google Scholar 

  17. G. Y. Tang, C. Yang, J. C. Chai, and H. Q. Gong, Int. J. Heat Mass Transfer, 2004, 47, 215.

    Article  CAS  Google Scholar 

  18. E. Grushka, R. M. McCormick, and J. J. Kirkland, Anal. Chem., 1989, 61, 241.

    Article  CAS  Google Scholar 

  19. X. C. Xuan, G. Q. Hu, and D. Q. Li, Electrophoresis, 2006, 27, 3171.

    Article  CAS  Google Scholar 

  20. S. P. Porras, E. Marziali, B. Gas, and E. Kenndler, Electrophoresis, 2003, 24, 1553.

    Article  CAS  Google Scholar 

  21. N. J. Petersen, R. P. Nikolajsen, K. B. Mogensen, and J. P. Kutter, Electrophoresis, 2004, 25, 253.

    Article  CAS  Google Scholar 

  22. X. C. Xuan and D. Q. Li, J. Micromech. Microeng., 2004, 14, 1171.

    Article  CAS  Google Scholar 

  23. J. P. Holman, “Heat Transfer”, 9th ed., 2002, McGraw-Hill Companies, Inc., New York.

  24. F. Kreith, R. F. Boehm, G. D. Raithby, K. G. T. Hollands, N. V. Suryanarayana, M. F. Modest, V. P. Carey, J. C. Chen, N. Lior, R. K. Shah, K. J. Bell, R. J. Moffat, A. F. Mills, A. E. Bergles, L. W. Swanson, V. W. Antonetti, T. F. Irvine, Jr., and M. Capobianchi, “Heat and Mass Transfer”, 1999, CRC Press LLC, Boca Raton.

    Google Scholar 

  25. A. Klinkenberg, “Gas Chromatography”, 1960, Butterworths Scientific Publications, London.

    Google Scholar 

  26. Y. Mechref, G. K. Ostrander, and Z. El Rassia, J. Chromatogr., A, 1997, 792, 75.

    Article  CAS  Google Scholar 

  27. W. H. Chen and C. Y. Liu, J. Chromatogr., A, 1999, 848, 401.

    Article  CAS  Google Scholar 

  28. Z. El Rassi, J. Postlewait, Y. Mechref, and G. K. Ostrander, Anal. Biochem., 1997, 244, 283.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. School of Material Science and Chemical Engineering, Tianjin Polytechnic University, 63 Chenglinzhuang Road, 300160, Tianjin, China

    Yugao Guo & James Jianmin Bao

  2. School of Pharmaceutical Science & Technology, Tianjin University, 92 Weijin Road, 300072, Tianjin, P. R. China

    Yugao Guo, Danning Liu, Huaifeng Wang & Ruijuan Yuan

Authors
  1. Yugao Guo
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  2. Danning Liu
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  3. Huaifeng Wang
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  4. Ruijuan Yuan
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  5. James Jianmin Bao
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Corresponding author

Correspondence to James Jianmin Bao.

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Guo, Y., Liu, D., Wang, H. et al. Zone Electrophoresis in an Inner-cooling Wide-bore Electrophoresis System with UV Detection. ANAL. SCI. 24, 1025–1030 (2008). https://doi.org/10.2116/analsci.24.1025

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  • DOI: https://doi.org/10.2116/analsci.24.1025

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