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Reactivity of Ferrocenylcarbodiimide with DNA Duplex Containing Single-mismatched Base Pairs

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Abstract

Ferrocenylcarbodiimide (1), which is known to react with a guanine (G) or thymine (T) base of single stranded DNA, was allowed to react with DNA duplex having a single mismatched base pair of G-T, T-T, or T-cytosine (C). Electrophoreograms of the reaction mixture showed that 1 could react with G or T base of the mismatched sites on the DNA duplex. However, 1 also reacted with the G base of the terminal site on the DNA duplex. This showed that 1 can react with an unpaired base or unstable base pair such as a terminal or mismatched base on the DNA duplex. Electrochemical mismatch detection could be achieved after hybridization of the ferrocenylated mismatched DNA duplex with a selected DNA probe-immobilized electrode. These results revealed that 1 has a potentiality of serving as a labeling reagent of mismatched bases on the DNA duplex, which is important in the search for heterozygous single nucleotide polymorphisms (SNPs).

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Authors and Affiliations

  1. Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, 819-0395, Japan

    Kosuke Mukumoto & Takahiko Nojima

  2. Department of Applied Chemistry, Faculty of Engineering, Kyushu Institute of Technology, Kitakyushu, 804-8550, Japan

    Sadayoshi Watanabe, Michinori Waki & Shigeori Takenaka

Authors
  1. Kosuke Mukumoto
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  2. Sadayoshi Watanabe
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  3. Takahiko Nojima
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  4. Michinori Waki
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  5. Shigeori Takenaka
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Corresponding author

Correspondence to Shigeori Takenaka.

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Mukumoto, K., Watanabe, S., Nojima, T. et al. Reactivity of Ferrocenylcarbodiimide with DNA Duplex Containing Single-mismatched Base Pairs. ANAL. SCI. 23, 645–649 (2007). https://doi.org/10.2116/analsci.23.645

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  • DOI: https://doi.org/10.2116/analsci.23.645

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