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Application of Alkaline Phosphatases from Different Sources in Pharmaceutical and Clinical Analysis for the Determination of Their Cofactors; Zinc and Magnesium Ions

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Abstract

Prospects of using different alkaline phosphatases bearing zinc and magnesium ions in their catalytic and allosteric sites, respectively, in pharmaceutical and clinical analysis were demonstrated. Also their application for the determination of zinc in insulin to control injection quality and magnesium in human urine for the diagnosis and treatment of magnesium deficiency was shown. The reaction of p-nitrophenyl phosphate hydrolysis was chosen as an indicator. The choice of appropriate alkaline phosphatase was substantiated, the influence of the nature of buffer solutions on the behavior of the enzyme-metal systems was studied, and the conditions of the indicator reaction proceeding in the presence of sample matrixes were optimized. Simple, rapid, sensitive, and selective enzymatic procedures for determining zinc and magnesium based on their inhibiting and activating effects on the catalytic activity of alkaline phosphatases from seal and chicken intestine, respectively, were developed.

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References

  1. T. G. Spiro, in “Inorganic Biochemistry”, ed. G. L. Eichorn, 1973, Vol. 1, Elsevier, Amsterdam, 549.

  2. H. N. Frenley, in “The enzymes”, ed. P. D. Boyer, 3rd ed., 1971, Vol. 4, Academic Press, New York, 417.

  3. D. W. Moss, Clin. Chem., 1982, 28, 2007.

    Article  CAS  PubMed  Google Scholar 

  4. S. Iino, Nippon Rinsho (in Japanese), 1995, 53, 1157.

    CAS  Google Scholar 

  5. J. A. Lorente, J. Morote, C. Raventos, G. Encabo, and H. Valnzuela, J. Urol., 1996, 155, 1348.

    Article  CAS  PubMed  Google Scholar 

  6. J. M. Van Emon, J. AOAC Int., 2001, 84, 125.

    Article  PubMed  Google Scholar 

  7. D. Trau, T. Theueri, M. Wilner, M. Meusel, and F. Spener, Biosens. Bioelectron., 1997, 12, 499.

    Article  CAS  PubMed  Google Scholar 

  8. S. V. Muginova, A. M. Zharvoronkova, and T. N. Shekhovtsova, J. Anal. Chem., 2005, 60, 218.

    Article  CAS  Google Scholar 

  9. T. N. Shekhovtsova and S. V. Muginova, Anal. Bioanal. Chem., 2005, 381, 1328.

    Article  CAS  PubMed  Google Scholar 

  10. T. N. Shekhovtsova, S. V. Muginova, I. A. Veselova, and M. A. Karkeshkin, in Proceedings of International Conference on Instrumental Methods of Analysis, Modern Trends and Applications, 2005, University of Crete, Heraklion, Crete, 245.

    Google Scholar 

  11. R. L. Dean, Biochem. Mol. Biol. Educ., 2002, 30, 401.

    Article  CAS  Google Scholar 

  12. R. Cloetens, Arch. Int. Pharmacodyn., 1942, 68, 419.

    CAS  Google Scholar 

  13. R. Cloetens, Arch. Int. Pharmacodyn., 1944, 69, 389.

    Google Scholar 

  14. R. A. Anderson, W. F. Bosron, F. S. Kennedy, and B. L. Vallee, Biochemistry, 1975, 72, 2989.

    CAS  Google Scholar 

  15. N. A. Bagirova, S. V. Muginova, T. N. Shekhovtsova, I. G. Gazaryan, and R. B. van Huystee, Anal. Lett., 2006, 39, 521.

    Article  CAS  Google Scholar 

  16. P. V. Bychkov, T. N. Shekhovtsova, and E. R. Milaeva, Bioinorg. Chem. Appl., 2005, 3, 191.

    Article  CAS  PubMed Central  Google Scholar 

  17. T. N. Shekhovtsova, N. A. Bagirova, and R. B. van Huystee, Talanta, 2001, 55, 1151.

    Article  PubMed  Google Scholar 

  18. T. N. Shekhovtsova, S. V. Muginova, J. A. Luchinina, and A. Z. Galimova, Anal. Chim. Acta, 2006, 573–574, 125.

    Article  PubMed  Google Scholar 

  19. P. V. Bychkov and T. N. Shekhovtsova, Mendeleev Commun., 2003, 2, 75.

    Google Scholar 

  20. M. A. Koupparis and P. I. Anaqnostopoulou, Analyst, 1986, 111, 1311.

    Article  CAS  PubMed  Google Scholar 

  21. S. W. Hovorka, H. Biesiada, T. D. Williams, A. Hühmer, and C. Schöneich, Pharm. Res., 2002, 19, 530.

    Article  CAS  PubMed  Google Scholar 

  22. I. D. Molger, A. C. Truttmann, R. O. von Vigier, A. Bettinelli, G. P. Ramelli, and M. G. Bianchetti, Pediatr. Nephrol., 1999, 13, 50.

    Article  Google Scholar 

  23. C. Wallace, Emergency Medicine, 2003, 15, 92.

    Article  PubMed  Google Scholar 

  24. M. M. Farajollahi, D. B. Cook, and C. H. Self, Iran. Biomed. J., 2002, 6, 105.

    CAS  Google Scholar 

  25. R. Cloetens, Biochem. Z., 1941, 307, 352.

    CAS  Google Scholar 

  26. A. M. Zharvoronkova, S. V. Muginova, and T. N. Shekhovtsova, Moscow University Bulletin, 2003, 44, 123.

    Google Scholar 

  27. R. M. C. Dawson, D. C. Elliott, W. H. Elliott, and K. M. Jons, “Data for Biochemical Research”, 1986, Clarendon Press, Oxford, 592.

    Google Scholar 

  28. A. Mildvan, in “The enzymes”, ed. P. D. Boyer, 3rd ed., 1971, Vol. 2, Academic Press, New York, 448.

  29. G. I. Tsizin, E. M. Sedykh, L. N. Bannykh, N. M. Sorokina, and Yu. A. Zolotov, J. Anal. Chem., 1995, 50, 72.

    CAS  Google Scholar 

  30. J. J. Jasaitis, V. J. Rasumas, and J. J. Kulys, Anal. Chim. Acta, 1983, 152, 271.

    Article  CAS  Google Scholar 

  31. V. P. Zhivopistsev and E. A. Selesneva, “Analyticheskaya Khimiya Tsinka (Analytical Chemistry of Zinc, in Russian)”, 1975, Nauka, Moscow.

    Google Scholar 

  32. T. N. Shekhovtsova, S. V. Muginova, and I. A. Veselova, Ross. Khim. Zh., 2004, XLVIII, 95.

    Google Scholar 

  33. D. L. Tsalev, “Atomic-Absorption Spectrometry in Occupational and Environmental Health Practice”, Vol. III, “Progress in Analytical Methodology”, 1995, CRC Press, Boca Raton, Florida, 349.

    Google Scholar 

  34. G. Armas, A. Cladera, E. Beccerra, J. M. Estela, and V. Cerda, Talanta, 2000, 52, 77.

    Article  PubMed  Google Scholar 

  35. T. N. Shekhovtsova, S. V. Chernetskaya, N. V. Belkova, and I. F. Dolmanova, J. Anal. Chem., 1994, 49, 709.

    Google Scholar 

  36. C. Gazzarrini, N. Stagni, P. Pollesello, P. D. Andrea, and B. De Bernard, Acta Diabetol. Lat., 1989, 26, 321.

    Article  CAS  PubMed  Google Scholar 

  37. C. K. Mann and J. H. Yoe, Anal. Chem., 1956, 28, 202.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Chemistry Department, M. V. Lomonosov Moscow State University, Leninskie Gory, Moscow, 119992 GSP-2, Russia

    Svetlana V. Muginova, Anna M. Zhavoronkova, Alexei E. Polyakov & Tatyana N. Shekhovtsova

Authors
  1. Svetlana V. Muginova
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  2. Anna M. Zhavoronkova
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  3. Alexei E. Polyakov
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  4. Tatyana N. Shekhovtsova
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Correspondence to Tatyana N. Shekhovtsova.

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Muginova, S.V., Zhavoronkova, A.M., Polyakov, A.E. et al. Application of Alkaline Phosphatases from Different Sources in Pharmaceutical and Clinical Analysis for the Determination of Their Cofactors; Zinc and Magnesium Ions. ANAL. SCI. 23, 357–363 (2007). https://doi.org/10.2116/analsci.23.357

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  • DOI: https://doi.org/10.2116/analsci.23.357

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