Skip to main content
SpringerLink
Log in

Structural Determination of Saponins Extracted from Starfish by Fast Atom Bombardment Collision-Induced Dissociation Mass Spectrometry

  • Notes
  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

Three saponins were extracted and isolated from starfish by reversed-phase high performance liquid chromatography (HPLC), and analyzed by fast atom bombardment mass spectrometry (FAB-MS). Their molecular weight information could be obtained by the presence of abundant [M+Na]+ ions and weak [M+H]+ ions in FAB-MS spectra. Moreover, high resolution mass measurements of their [M+Na]+ ions were performed at the resolution of 10000 to elucidate the element composition of extracted saponins. The collision-induced dissociation (CID) of sodium-adducted molecules [M+Na]+ yielded diverse product ions via dissociated processes. In the collision-induced dissociation (CID)-MS/MS analysis of [M+Na]+ ion, the sulfate-containing saponins produced characteristic ions such as SO4Na+, [NaHSO4+Na]+, [M+Na-sugar]+ and [M+Na-2sugar]+ ions, whereas the sulfate-free compound showed characteristic ions produced by cleavage of sugar moiety and side chain of aglycone. The fragmentation patterns could provide information on the linkage position of sugar groups in aglycone and sulfate groups.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. K. Hostettmann and A. Marston, “Saponins”, 1995, Cambridge University Press, Cambridge, 1.

    Book  Google Scholar 

  2. G. R. Fenwick, K. R. Price, C. Tsukamoto, and K. Okubo, “Toxic Substances in Crop Plants”, ed. J. P. F. D’Mello, C. M. Duffus, and J. H. Duffus, 1991, The Royal Society of Chemistry, Cambridge, 285.

  3. M.-R. Lee, C.-M. Chen, B.-H. Hwang, and L.-M. Hsu, J. Mass Spectrom., 1999, 34, 804.

    Article  CAS  Google Scholar 

  4. M. Cui, W. Sun, F. Song, Z. Liu, and S. Liu, Rapid Commun. Mass Spectrom., 1999, 13, 873.

    Article  CAS  Google Scholar 

  5. D. C. van Setten, G. J. ten Hove, E. J. H. J. Wiertz, J. P. Kamerling, and G. van de Werken, Anal. Chem., 1998, 70, 4401.

    Article  Google Scholar 

  6. H. D. Chludil, A. M. Seldes, and M. S. Maier, J. Nat. Prod., 2002, 65, 153.

    Article  CAS  Google Scholar 

  7. X.-G. He, J. Chromatogr., A, 2000, 880, 203.

    Article  CAS  Google Scholar 

  8. S. Fang, C. Hao, W. Sun, Z. Liu, and S. Liu, Rapid Commun. Mass Spectrom., 1998, 12, 589.

    Article  CAS  Google Scholar 

  9. C. Perret, J.-L. Wolfender, and K. Hostettmann, Phytochem. Anal., 1999, 10, 272.

    Article  CAS  Google Scholar 

  10. M. Iorizzi and R. Riccio, “Naturally Occurring Glycosides”, ed. R. Ikan, 1999, John Wiley and Sons, Chichester, 345.

  11. A. Casapullo, E. Finamore, L. Minale, F. Zollo, J. B. Carré, C. Debitus, D. Laurent, A. Folgore, and F. Galdiero, J. Nat. Prod., 1993, 56, 105.

    Article  CAS  Google Scholar 

  12. A. J. Roccatagliata, M. S. Maier, A. M. Seldes, M. Iorizzi, and L. Minale, J. Nat. Prod., 1994, 57, 747.

    Article  Google Scholar 

  13. N. Fusetani, Y. Kato, K. Hashimoto, T. Komori, Y. Itakura, and T. Kawasaki, J. Nat. Prod., 1984, 47, 997.

    Article  CAS  Google Scholar 

  14. M. S. Maier, A. Roccatagliata, and A. M. Seldes, J. Nat. Prod., 1993, 56, 939.

    Article  CAS  Google Scholar 

  15. M. Iorizzi, L. Minale, R. Riccio, M. Debray, and J. L. Menou, J. Nat. Prod., 1986, 49, 67.

    Article  CAS  Google Scholar 

  16. S. de Marino, M. Iorizzi, E. Palagiano, F. Zollo, and C. Roussakis, J. Nat. Prod., 1998, 61, 1319.

    Article  Google Scholar 

  17. R. Higuchi, Y. Tokimitsu, and T. Komori, Phytochemistry, 1988, 27, 1165.

    Article  CAS  Google Scholar 

  18. C. Lavaud, G. Massiot, L. Le Men-Olivier, A. Viari, P. Vigny, and C. Delaude, Phytochemistry, 1992, 31, 3177.

    Article  CAS  Google Scholar 

  19. R. Higuchi and T. Komori, Phytochemistry, 1987, 26, 2357.

    Article  CAS  Google Scholar 

  20. Y. Z. Chen, N. Y. Chen, H. Q. Li, F. Z. Zhao, and N. Chen, Biomed. Environ. Mass Spectrom., 1987, 14, 9.

    Google Scholar 

  21. T. W. D. Chan, P. P. H. But, S. W. Cheng, I. M. Y. Kwok, F. W. Lau, and H. X. Xu, Anal. Chem., 2000, 72, 1281.

    Article  CAS  Google Scholar 

  22. M. Cui, F. Song, Z. Liu, and S. Liu, Rapid Commun. Mass Spectrom., 2001, 15, 586.

    Article  CAS  Google Scholar 

  23. W. Wang, F. Li, N. Alam, Y. Liu, J. Hong, C. K. Lee, K. S. Im, and J. H. Jung, J. Nat. Prod., 2002, 65, 1649.

    Article  CAS  Google Scholar 

  24. M. R. Lee, J. S. Lee, J. C. Wang, and G. R. Waller, “Saponins Used in Food and Agriculture”, ed. G. R. Waller and K. Yamasaki, 1996, Plenum Press, New York, 331.

Download references

Author information

Authors and Affiliations

  1. Hazardous Substance Research Team, Korea Basic Science Institute, Seoul, 136-701, Korea

    Ji Hye Gil

  2. College of Pharmacy, Pusan National University, Pusan, 609-735, Korea

    Jee H. Jung

  3. Department of Chemistry, University of Korea, Seoul, 136-701, Korea

    Kang-Jin Kim

  4. College of Pharmacy, Kyung Hee University, Hoegi-Dong, Dongdaemoon-Ku, Seoul, 130-701, Korea

    Min-Sun Kim & Jongki Hong

Authors
  1. Ji Hye Gil
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jee H. Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kang-Jin Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Min-Sun Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jongki Hong
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jongki Hong.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gil, J.H., Jung, J.H., Kim, KJ. et al. Structural Determination of Saponins Extracted from Starfish by Fast Atom Bombardment Collision-Induced Dissociation Mass Spectrometry. ANAL. SCI. 22, 641–644 (2006). https://doi.org/10.2116/analsci.22.641

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.22.641

Search

Navigation