Abstract
A simple and rapid quantification method was developed for determining nisoldipine in plasma. After a simple protein precipitation with a mixture of 10% of zinc sulfate and methanol, the analytes were chromatographed on a reversed-phase C18 column, and detected by MS/MS. The assay precision was less than 10.7%, and the accuracy ranged from 86 to 112%. The limit of quantification was 0.1 ng/ml. This method was used to measure the plasma concentration of nisoldipine from healthy subjects after a single 5-mg oral dose of nisoldipine.
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References
J. van Harten, P. van Brummelon, R. R. Zeegers, M. Danhof, and D. Breimer, Br. J. Clin. Pharmacol., 1988, 25, 709.
Z. Zhang, Y. Tian, L. Ni, and J. Li, J. Chromatogr. Sci., 2004, 42, 501.
R. Heinig, V. Muschalek, and G. Ahr, J. Chromatogr., B, 1994, 655, 286.
M. P. Marques, N. A. Santos, E. B. Coelho, P. S. Bonato, and V. L. Lanchote, J. Chromatogr., B, 2001, 762, 87.
C. Polson, P. Sarkar, B. Incledon, V. Raguvaran, and R. Crant, J. Chromatogr., B, 2003, 785, 263.
L. H. Miglioranca, R. E. Barrientos-Astigarraga, B. S. Schug, H. H. Blume, A. S. Pereira, and G. De Nucci, J. Chromatogr., B, 2005, 814, 217.
H. Wang, L. Zhang, Q. Wang, Z. Yuan, and M. Li, J. Chromatogr., B, 2006, 835, 71.
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Kang, W. Simple and Sensitive Determination of Nisoldipine in Plasma Using Liquid Chromatography-Tandem Mass Spectrometry. ANAL. SCI. 22, 1597–1599 (2006). https://doi.org/10.2116/analsci.22.1597
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DOI: https://doi.org/10.2116/analsci.22.1597