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Selenium Species Determination in Selenium-Enriched Pumpkin (Cucurbita pepo L.) Seeds by HPLC-UV-HG-AFS

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Abstract

Pumpkins were treated by spraying the leaves in the flowering period with a water solution containing 1.5 mg Se per liter in the form of Na2SeO4. The average total selenium content of seeds was found to be 0.19 μg g-1 in nontreated pumpkins and 1.1 μg g-1 in exposed ones. For speciation analysis, enzymatic hydrolysis with different amounts of Protease XIV was carried out. Under optimal conditions of enzymatic hydrolysis, 90% of the total selenium was found in soluble forms. Separation of species was performed using HPLC on anion and cation exchange columns and for detection UV-HG-AFS was applied. In enzymatic hydrolysis extracts, the main fraction of selenium was bound as selenomethionine (SeMet), representing on average of 81 ± 8% of the total Se content in the sample.

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Authors and Affiliations

  1. Jžzef Stefan Institute, Jamova 39, Ljubljana, Slovenia

    Polona Smrkolj & Vekoslava Stibilj

  2. Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, Ljubljana, Slovenia

    Ivan Kreft

  3. Department of Applied Chemistry, Faculty of Food Science, Corvinus University of Budapest, Villdnyiut 29-33, Budapest, Hungary

    Emese Kapolna

Authors
  1. Polona Smrkolj
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  2. Vekoslava Stibilj
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  3. Ivan Kreft
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  4. Emese Kapolna
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Correspondence to Vekoslava Stibilj.

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Smrkolj, P., Stibilj, V., Kreft, I. et al. Selenium Species Determination in Selenium-Enriched Pumpkin (Cucurbita pepo L.) Seeds by HPLC-UV-HG-AFS. ANAL. SCI. 21, 1501–1504 (2005). https://doi.org/10.2116/analsci.21.1501

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  • DOI: https://doi.org/10.2116/analsci.21.1501

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