Abstract
We describe here a method of affinity capillary electrophoresis in which oligodeoxynucleotide (ODN) was immobilized onto the inner surface of the capillary. The immobilized ODN functioned successfully as an affinity ligand for sequence-based DNA separation. Six- or 12-mer ODN with a sequence complementary to one of the c-K-ras gene was used as an immobilized ligand. When the 12-mer ODN was used, the detection peak for the complementary ODN disappeared selectively, while the single-base mutant was detected as usual. In contrast, when the 6-mer ODN was used as the affinity ligand with a mixture of the complementary ODN and its single-base mutant, it was possible to detect both as completely separate peaks. That is, the separation mode was dependent on the base number of the immobilized ODN used as an affinity ligand.
Similar content being viewed by others
References
A. J. Brookes, Gene, 1999, 234, 177.
N. Risch and K. Merikangas, Science, 1996, 273, 1516.
U. Landegren, M. Nilsson, and P. Y. Kwok, Genome Res., 1998, 8, 769.
P. G. Righetti and C. Gelfi, J. Biochem. Biophys. Methods, 1999, 41, 75.
L. S. Kutach, S. Bolshakov, and H. N. Ananthaswamy, Electrophoresis, 1999, 20, 1204.
K. J. Ulfelder, H. E. Schwaltz, J. M. Hall, and F. J. Sunzeri, Anal. Biochem., 1992, 200, 260.
J. Ren, J. Chromatogr. B, 2000, 741, 115.
D. J. Harrison, K. Fluri, K. Seiler, Z. Fan, C. S. Effenhauser, and A. Manz, Science, 1993, 261, 895.
G. J. M. Bruin, Electrophoresis, 2000, 21, 3931.
S. C. Jacobson and J. M. Ramsey, Anal. Chem., 1996, 68, 720.
C. S. Effenhauser, A. Paulus, A. Manz, and H. M. Widmer, Anal. Chem., 1994, 66, 2949.
A. T. Wooley and R. A. Mathies, Proc. Natl. Acad. Sci. USA, 1994, 91, 11348.
M. T. Lam, Q. H. Wan, C. A. Boulet, and X. C. Le, J. Chromatogr. A, 1999, 854, 545.
Q. H. Wan and X. C. Le, Anal. Chem., 2000, 72, 5583.
V. Pavski and X. C. Le, Anal. Chem., 2001, 73, 6070.
Y. Baba, M. Tsuhako, T. Sawa, M. Akashi, and E. Yashima, Anal. Chem., 1992, 64, 1920.
A. Muscate, F. Natt, A. Paulus, and M. Ehrat, Anal. Chem., 1998, 70, 1419.
Y. Katayama, T. Arisawa, Y. Ozaki, and M. Maeda, Chem. Lett., 2000, 106.
T. Anada, T. Arisawa, Y. Ozaki, T. Takarada, Y. Katayama, and M. Maeda, Electrophoresis, 2002, 23, 2267.
Y. Ozaki, Y. Katayama, T. Ihara, and M. Maeda, Anal. Sci., 1999, 15, 389.
T. Anada, Y. Ozaki, T. Takarada, Y. Katayama, and M. Maeda, Anal. Sci., 2001, 17 (supplement), i1311.
C. R. Cantor, M. M. Warshaw, and H. Shapiro, Biopolymers, 1970, 9, 1059.
S. J. Hjerten, Chromatogr., 1985, 347, 191.
K. Shimizu, M. Goldfarb, Y. Suard, M. Perucho, Y. Li, T. Kamata, J. Feramisco, E. Stavnezer, J. Fogh, and M. H. Wigler, Proc. Natl. Acad. Sci. USA, 1983, 80, 2112.
A. Hall, C. J. Marshall, N. K. Spurr, and R. A. Weiss, Nature, 1983, 303, 396.
H. Land, L. F. Parada, and R. A. Weinberg, Science, 1983, 222, 771.
A. Z. Li, H. Huang, X. Re, L. J. Qi, and K. A. Marx, Biophys. J., 1998, 74, 964.
G. S. Manning, J. Phys. Chem., 1981, 85, 1508.
H. Nenner and C. H. Zimmer, Biopolymers, 1966, 4, 321.
H. Krakauer, Biopolymers, 1971, 4, 321.
H. Krakauer, Biochemistry, 1974, 13, 2579.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Anada, T., Ogawa, M., Yokomizo, H. et al. Oligodeoxynucleotide-Modified Capillary for Electrophoretic Separation of Single-Stranded DNAs with a Single-Base Difference. ANAL. SCI. 19, 73–77 (2003). https://doi.org/10.2116/analsci.19.73
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.2116/analsci.19.73