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Spectrophotometric Assay of Yeast Vitality Using 2,3,5,6-Tetramethyl-1,4-benzoquinone and Tetrazolium Salts

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Abstract

A method for the spectrophotometric assay of yeast vitality was developed using 2,3,5,6-tetramethyl-1,4-benzoquinone and tetrazolium salts. The metabolic efficiency of 2,3,5,6-tetramethyl-1,4-benzoquinone by yeast cells was used as an index of yeast vitality. 2,3,5,6-Tetramethyl-1,4-benzoquinone was reduced to 2,3,5,6-tetramethyl-1,4-hydroquinone by yeast cells. Then, the superoxide anion radicals generated from O2 by reduction with 2,3,5,6-tetramethyl-1,4-hydroquinone under alkaline conditions reduced tetrazolium salts to formazan, which exhibited absorbance maxima at 440 nm. A linear relationship between the absorbance and viable cell density was obtained in the range of 1.0 × 105-2.0 × 107 cells/ml for a sample solution. During the cultivation of yeast cells, the absorbance showed almost an anti-parallel change with that of glucose in yeast growth and fermentation, suggesting that the absorbance change reflected the vitality of yeast cells.

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Authors and Affiliations

  1. Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, Kurume, 839-0861, Japan

    Tadayuki Tsukatani & Takahiro Oba

  2. Department of Bioresources Science, Faculty of Agriculture, Kochi University, Nankoku, 783-8502, Japan

    Hiroyuki Ukeda

  3. Division of Food Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School Kyushu University, Fukuoka, 812-8581, Japan

    Kiyoshi Matsumoto

Authors
  1. Tadayuki Tsukatani
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  2. Takahiro Oba
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  3. Hiroyuki Ukeda
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  4. Kiyoshi Matsumoto
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Correspondence to Tadayuki Tsukatani.

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Tsukatani, T., Oba, T., Ukeda, H. et al. Spectrophotometric Assay of Yeast Vitality Using 2,3,5,6-Tetramethyl-1,4-benzoquinone and Tetrazolium Salts. ANAL. SCI. 19, 659–664 (2003). https://doi.org/10.2116/analsci.19.659

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  • DOI: https://doi.org/10.2116/analsci.19.659

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