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A novel Bod-mediator biosensor based on Ferrocene and Debaryomyces hansenii yeast cells

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Abstract

The mediators ferrocene, 1,1'-dimethylferrocene, ferrocene carboxaldehyde, ferrocene acetonitrile, neutral red, 2,6-dichlorophenolindophenol, thionine, methyl blue, and potassium ferricyanide were used in combination with Debaryomyces hansenii yeast cells to create a biosensor receptor for biochemical oxygen demand (BOD) assays. In this eukaryote, ferrocene and neutral red were observed to efficiently transfer electrons. The biosensor based on ferrocene was characterized by long-term stability (39 days) and a wide range of substrate specificity. The lower detected concentration boundary was 25.2 mg O2/dm3. A high correlation (R = 0.9971) was observed between the results obtained with water samples by this approach and the standard method. This is the first attempt to create a combination of yeast cells and a mediator. The biosensor can be employed for further research on the possibilities of its conventional use.

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Authors and Affiliations

  1. Tula State University, Tula, 300012, Russia

    A. S. Zaitseva, V. A. Arlyapov, N. Yu. Yudina, N. M. Nosova, V. A. Alferov & A. N. Reshetilov

  2. G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    A. N. Reshetilov

Authors
  1. A. S. Zaitseva
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  2. V. A. Arlyapov
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  3. N. Yu. Yudina
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  4. N. M. Nosova
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  5. V. A. Alferov
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  6. A. N. Reshetilov
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Correspondence to A. S. Zaitseva.

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Original Russian Text © A.S. Zaitseva, V.A. Arlyapov, N.Yu. Yudina, N.M. Nosova, V.A. Alferov, A.N. Reshetilov, 2017, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2017, Vol. 53, No. 3, pp. 341–348.

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Zaitseva, A.S., Arlyapov, V.A., Yudina, N.Y. et al. A novel Bod-mediator biosensor based on Ferrocene and Debaryomyces hansenii yeast cells. Appl Biochem Microbiol 53, 381–387 (2017). https://doi.org/10.1134/S0003683817030152

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  • DOI: https://doi.org/10.1134/S0003683817030152

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