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A Novel Solution-auto-introduction Electrophoresis Microchip Based on Capillary Force

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Abstract

A novel solution-auto-introduction electrophoresis microchip based on capillary force aimed at improving portability is proposed in this paper. Two kinds of materials with micropores, poly(vinyl alcohol) (PVA)-sponge and nano-sponge, were employed as suction pumps that realized the introduction of a running buffer into the reservoirs. The surfaces of the microchannels in the microchips were modified by PVA to improve the moving velocity of the running buffer and the detection performance of the microchip. The introduction velocity of a running buffer in the PVA-coated microchannels was increased by two times compared with that in the native microchannels. The electrophoresis detection performance of several microchips composed of different microchannels and suction materials were evaluated comparatively. The results indicated that the surface coating of PVA can significantly improve the repeatability of the detection results by 20–40%, and the noise of the detected signals in the PVA-coated microchips is much lower than that in the native microchips. The proposed solution-auto-introduction electrophoresis microchip is a successful attempt that completely avoids the external connectors to accomplish the auto-introduction of running buffer. The solution-auto-introduction method provides a new train of thought for portable detection instruments with electrophoresis microchips in the future.

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References

  1. F. Kitagawa, T. Kawai, and K. Otsuka, Anal. Sci., 2013, 29, 1129.

    Article  CAS  PubMed  Google Scholar 

  2. T. G. Henares, S. Funano, K. Sueyoshi, T. Endo, and H. Hisamoto, Anal. Sci., 2014, 30, 7.

    Article  CAS  PubMed  Google Scholar 

  3. F. Kitagawa, S. Nakagawara, I. Nukatsuka, Y. Hori, K. Sueyoshi, and K. Otsuka, Anal. Sci., 2015, 31, 1171.

    Article  CAS  PubMed  Google Scholar 

  4. Y. Shimada and T. Kaneta, Anal. Sci., 2018, 34, 65.

    Article  CAS  PubMed  Google Scholar 

  5. I. Charhrouchni, A. Pallandre, I. L. Potier, C. Deslouis, and A. M. Haghiri-Gosnet, Electrophoresis, 2013, 34, 725.

    Article  CAS  PubMed  Google Scholar 

  6. X. Yan, W. Liu, Y. Yuan, and C. Chen, Anal. Methods, 2015, 7, 5295.

    Article  CAS  Google Scholar 

  7. Z. Xu, X. Wang, R. J. Weber, R. Kumar, and L. Dong, IEEE Sens. J., 2017, 17, 4330.

    Article  CAS  Google Scholar 

  8. A. Floris, S. Staal, S. Lenk, E. Staijen, D. Kohlheyer, J. Eijkel, and A. Berg, Lab Chip, 2010, 10, 1799.

    Article  CAS  PubMed  Google Scholar 

  9. K. Ansari, J. Y. S. Ying, P. C. Hauser, N. F. Rooij, and I. Rodriguez, Electrophoresis, 2013, 34, 1390.

    Article  CAS  PubMed  Google Scholar 

  10. M. Smolka, D. Puchberger-Enengl, M. Bipoun, A. Klasa, M. Kiczkajlo, W. Smiechowski, P. Sowinski, C. Krutzler, F. Keplinger, and M. J. Vellekoop, Precis. Agric., 2017, 18, 152.

    Article  Google Scholar 

  11. A. J. Gaudry, Y. H. Nai, R. M. Guijt, and M. C. Breadmore, Anal. Chem., 2014, 86, 3380.

    Article  CAS  PubMed  Google Scholar 

  12. L. J. Cheng and H. C. Chang, Lab Chip, 2014, 14, 979.

    Article  CAS  PubMed  Google Scholar 

  13. T. Knipfer, I. F. Cuneo, J. M. Earles, C. Reyes, C. R. Brodersen, and A. J. McElronea, Plant Physiol., 2017, 175, 1649.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. W. Wu and A. Manz, RSC Adv, 2015, 5, 70737.

    Article  CAS  Google Scholar 

  15. K. Yong-Hoon and T. Urisu, Appl. Surf. Sci., 2011, 257, 9314.

    Article  Google Scholar 

  16. T. J. Clark, P. H. McPherson, and K. F. Buechler, Point of Care, 2002, 1, 42.

    Article  Google Scholar 

  17. J. Liu, J. Wang, Z. Chen, Y. Yu, X. Yang, X. Zhang, Z. Xu, and C. Liu, Lab Chip, 2011, 11, 969.

    Article  CAS  PubMed  Google Scholar 

  18. M. Yang, Z. Huang, and H. You, Soc. Open Sci., 2018, 5, 171687.

    Article  Google Scholar 

  19. H. Yu, Z. Z. Chong, S. B. Tor, E. Liu, and N. H. Loh, RSC Adv., 2015, 5, 8377.

    Article  CAS  Google Scholar 

  20. H. Tsai, R. J. Yang, C. H. Tai, and L. M. Fu, Electrophoresis, 2005, 26, 674.

    Article  CAS  PubMed  Google Scholar 

  21. H. Sakamoto, R. Hatsuda, K. Miyamura, and S. Sugiyama, Micro Nano Lett., 2012, 7, 64.

    Article  CAS  Google Scholar 

  22. C. R. Crick and I. P. Parkin, Thin Solid Films, 2011, 519, 2181.

    Article  CAS  Google Scholar 

  23. M. L. Hupert, W. J. Guy, S. D. Llopis, H. Shadpour, S. Rani, D. E. Nikitopoulos, and S. A. Soper, Microfluid. Nanofluid., 2007, 3, 1.

    Article  CAS  Google Scholar 

  24. T. Yasui, M. R. Mohamadi, N. Kaji, Y. Okamoto, M. Tokeshi, and Y. Baba, Biomicrofluidics, 2011, 5, 044114.

    Article  PubMed Central  Google Scholar 

  25. H. Yoshiki, A. Oki, H. Ogawa, and Y. Horiike, J. Vac. Sci. Technol., A, 2002, 20, 24.

    Article  CAS  Google Scholar 

  26. N. Vourdas, A. Tserepi, A. G. Boudouvis, and E. Gogolides, Microelectron. Eng., 2008, 85, 1124.

    Article  CAS  Google Scholar 

  27. M. Mori, F. Ishikawara, T. Tomoda, S. Yamada, M. Okamoto, H. Itabashi, Y. Seki, R. Matsumoto, Y. Shoho, L. Martha, H. Sumino, and M. Murakami, J. Chromatogr. B, 2016, 1012-1013, 178.

    Article  Google Scholar 

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Acknowledgments

We are grateful for the funding support from the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (No. SQ2015BA0800060) and the Science and Technology Research Project of Anhui Province, China (No. 1501031088).

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Authors and Affiliations

  1. Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, 230031 Anhui, China

    Mingpeng Yang, Zhe Huang, Jianguo Chang & Hui You

  2. University of Science and Technology of China, Hefei, 230026 Anhui, China

    Mingpeng Yang, Zhe Huang, Jianguo Chang & Hui You

Authors
  1. Mingpeng Yang
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  2. Zhe Huang
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  3. Jianguo Chang
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  4. Hui You
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Correspondence to Hui You.

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Yang, M., Huang, Z., Chang, J. et al. A Novel Solution-auto-introduction Electrophoresis Microchip Based on Capillary Force. ANAL. SCI. 34, 1285–1290 (2018). https://doi.org/10.2116/analsci.18P199

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  • DOI: https://doi.org/10.2116/analsci.18P199

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