Abstract
The electrochemical reduction mechanism of ethidium bromide was first studied by spectroelectrochemistry. This reduction was proved to be a two-step process by cyclic voltammetry, differential pulse voltammetry and spectroelectrochemistry, in which each step was proved to be a one-electron transfer process by a spectropotentiostatic fluorescence technique. Hydroethidine was confirmed to be the final product by comparing the spectrum of the product of the electrochemical reduction to that of the product of the chemical reduction of ethidium bromide, and a carbon-centered radical was concluded to be a reasonable intermediate product during the electrochemical reduction of ethidium bromide.
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M. Feng, Y. L. Yang, P. G. He, and Y. Z. Fang, Spectrochim. Acta Part A, 2000, 56, 581.
G. F. Chen, H. Y. Qu, P. G. He, and Y. Z. Fang, Chem. World, 2000, S1, 158.
P. J. Kulesza, M. A. Malik, A. Denca, and J. Strojek, Anal. Chem., 1996, 68, 2442.
M. J. Shaw and W. E. Geiger, Organometallics, 1996, 15, 13.
P. Rapta and L. Dunsch, J. Electroanal. Chem., 2001, 507, 287.
D. H. Jones and A. S. Hinman, Can. J. Chem., 1996, 74, 1403.
J. Losada, I. DelPeso, and L. Beyel, Inorg. Chim. Acta, 2001, 321, 107.
P. Rapta and L. Dunsch, J. Electroanal. Chem., 2001, 507, 287.
M. Feng, D. J. Long, and Y. Z. Fang, Anal. Chim. Acta, 1998, 363, 67.
H. Y. Chen and Y. T. Long, Anal. Chim. Acta, 1999, 382, 171.
Y. C. Zhu, G. J. Cheng, and S. J. Dong, Biophys. Chem., 2000, 87, 103.
I. Taniguchi, T. Fujiwara, and M. Tominaga, Chem. Lett., 1992, 12, 1217.
T. Y. Zhou and J. S. Yu, Chem. J. Chin. Univ., 1998, 19, 207.
M. J. Waring, Biochim. Biophys. Acta, 1964, 87, 358.
M. J. Waring, J. Mol. Biol., 1965, 13, 269.
W. Bauer and J. Vinograd, J. Mol. Biol., 1968, 33, 141.
G. P. Kreishman, S. I. Chan, and W. Baucer, J. Mol. Biol., 1971, 61, 45.
X. G. Sun, C. E. Cao, Y. J. He, and J. F. Qin, Sci. China Series B, 1999, 42, 62.
S. Neidle and Z. Abraham, CRC Crit. Rev. Biochem., 1984, 17, 73.
D. J. Patel and L. I. Kanuel, Proc. Natl. Acad. Sci. U.S.A., 1976, 73, 3343.
C. C. Tsai, S. C. Jain, and H. M. Sobel, J. Mol. Biol., 1977, 114, 301.
J. B. Lepecq and C. Paoletti, J. Mol. Biol., 1967, 27, 87.
J. Olmsted, Biochemistry, 1977, 16, 3647.
D. A. Vondyke and H. Y. Cheng, Anal. Chem., 1989, 61, 633.
C. Xu, P. G. He, and Y. Z. Fang, Chem. J. Chin. Univ., 2000, 21, 1187.
D. M. Anjo, M. Kahr, M. M. Khodabakhsh, and S. Nowinski, Anal. Chem., 1989, 61, 2603.
G. Thomas and B. Roques, Fed. Eur. Biochem. Soc. Lett., 1972, 26, 169.
P. M. Gallop, M. A. Paz, E. Henson, and S. A. Latt, Biotechniques, 1984, 3, 32.
V. P. Bindokas, J. Jordan, C. C. Lee, and R. J. Miller, J. Neurosci., 1996, 16, 1324.
L. Benov, L. Sztejnberg, and I. Fridovich, Free Radical Biol. Med., 1998, 25, 826.
M. Gomberg, Ber., 1900, 33, 3150.
S. Jockusch, T. Hirano, Z. Q. Liu, and N. J. Turro, J. Phys. Chem. B, 2000, 104, 1212.
N. J. Turro, A. McDermott, X. G. Lei, W. Li, L. Abrams, M. F. Ottaviani, H. S. Beard, K. N. Houk, B. R. Beno, and P. S. Lee, Chem. Commun., 1998, 697.
T. Hirano, W. Li, L. Abrams, P. J. Krusic, M. F. Ottaviani, and N. J. Turro, J. Am. Chem. Soc., 1999, 121, 7170.
T. Hirano, W. Li, L. Abrams, P. J. Krusic, M. F. Ottaviani, and N. J. Turro, J. Org. Chem., 2000, 65, 1319.
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Hu, X., Wang, Q., He, P. et al. Spectroelectrochemistry Study on the Electrochemical Reduction of Ethidium Bromide. ANAL. SCI. 18, 645–650 (2002). https://doi.org/10.2116/analsci.18.645
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DOI: https://doi.org/10.2116/analsci.18.645