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Investigation of the Chemico-physical Characteristics of the Active Components in the Chinese Herb Gastrodia elata Bl. by Capillary Zone Electrophoresis

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Abstract

A CE method for investigating the chemico-physical characteristics of the active components of low molecular weight in Gastrodia elata Bl. is described. First, the dissociation constants of five active components were determined based on the relation between the effective mobility of the solutes and the buffer pH value. Second, an equation that describes the relation of the migration time and the molecular weight was developed and used to predict the migration order and to calculate the electroosmotic velocity. The results predicted by theory agreed well with that from experiments.

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Authors and Affiliations

  1. Department of Chemistry, Hebei University, Baoding, 071002, P. R. China

    Dexian Wang, Gengliang Yang, Baohui Li & Zhiwei Li

  2. Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Beijing, 100080, P. R. China

    Yi Chen

  3. Hebei Medical College for Continue Education, Baoding, 071000, P. R. China

    Dexian Wang

Authors
  1. Dexian Wang
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  2. Gengliang Yang
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  3. Baohui Li
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  4. Zhiwei Li
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  5. Yi Chen
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Correspondence to Gengliang Yang.

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Wang, D., Yang, G., Li, B. et al. Investigation of the Chemico-physical Characteristics of the Active Components in the Chinese Herb Gastrodia elata Bl. by Capillary Zone Electrophoresis. ANAL. SCI. 18, 409–412 (2002). https://doi.org/10.2116/analsci.18.409

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  • DOI: https://doi.org/10.2116/analsci.18.409

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