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Impact of Saliva Collection and Processing Methods on Aspartate Aminotransferase, Creatin Kinase and Lactate Dehydrogenase Activities

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Abstract

We aimed to investigate the impact of saliva collection and processing methods on AST, CK and LDH. Saliva was collected from 17 healthy participants by a passive drool. Each saliva sample was distributed into 3 aliquots: not treated, centrifuged, and passed through cotton. Centrifugation improved the precision of assays and produced lower values of AST and CK. The use of cotton resulted in decreased levels of LDH. This data stress the importance of the standardization of sample processing to measure enzymes in saliva.

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Acknowledgments

The authors acknowledge Isabel Rodriguez for her technical assistance. Financial support was provided by the Program “Juan de la Cierva” of “Ministerio de Economia y Competitividad”, Spain, through a postdoctoral grant. In addition, the Seneca Foundation of Murcia Region and García Cugat Foundation supported this research.

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Authors and Affiliations

  1. Interdisciplinary Laboratory, Interlab-UMU, Campus of Excellence Mare Nostrum, University of Murcia, Spain

    Tomás Barranco, Jose J. Cerón, Pía López-Jornet, Fernando Tecles & Asta Tvarijonaviciute

  2. Departament de Medicina i Cirugia Animals, Universidad Autonoma de Barcelona, Barcelona, Spain

    Josep Pastor

  3. Department of Animal Medicine and Surgery, Universidad CEU Cardenal Herrera, Edificio Seminario s/n, 46113, Moncada, Valencia, Spain

    Jose M. Carrillo & Mónica Rubio

  4. Clinical Analysis, University Hospital "Virgen de la Arixaca", Murcia, Spain

    Pedro L. Tornel

  5. Department of Orthopaedic Surgery and Traumatology, Hospital Quirón, Plaça d'Alfonso Comin, 5-7 08023, Spain, Barcelona

    Ramón Cugat

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  1. Tomás Barranco
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  2. Jose J. Cerón
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  3. Pía López-Jornet
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  4. Josep Pastor
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  5. Jose M. Carrillo
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  6. Mónica Rubio
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  7. Pedro L. Tornel
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  8. Ramón Cugat
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  9. Fernando Tecles
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  10. Asta Tvarijonaviciute
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Corresponding author

Correspondence to Fernando Tecles.

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Barranco, T., Cerón, J.J., López-Jornet, P. et al. Impact of Saliva Collection and Processing Methods on Aspartate Aminotransferase, Creatin Kinase and Lactate Dehydrogenase Activities. ANAL. SCI. 34, 619–622 (2018). https://doi.org/10.2116/analsci.17N035

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  • DOI: https://doi.org/10.2116/analsci.17N035

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