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Spectrophotometric Determination of Ascorbic Acid with Iron(III) and p-Carboxyphenylfluorone in a Cationic Surfactant Micellar Medium

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Abstract

A simple and highly sensitive spectrophotometric method for the determination of ascorbic acid (AA) was established by using iron(III) and p-carboxyphenylfluorone (PCPF) in a cationic surfactant micellar medium. The apparent molar absorptivity of the proposed method, which does not require an extraction procedure, was 2.05 × 106 dm3 mol–1 cm–1 at 655 nm. Beer’s law was obeyed in the concentration range of 0.02–0.12 µg/cm3 for AA. The procedure was successfully applied to assays of AA in pharmaceutical preparations. It is suggested that the method is based on a coupled redox-complexation reaction in which the first step is the oxidation of AA by iron(III), and the second step includes the formations of the iron(II)-PCPF (1:2) complex and the dehydroascorbic acid-iron(III)-PCPF (1:1:2) complex.

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References

  1. E. Kimoto, “The l-ascorbate cascade”, 1994, Kaisei Shuppan, Tokyo.

    Google Scholar 

  2. L. A. Pachla and D. L. Reynolds, J. Assoc. Off. Anal. Chem., 1985, 68, 1.

    CAS  PubMed  Google Scholar 

  3. S. P. Arya, M. Mahajan, and P. Jain, Anal. Sci., 1998, 14, 889.

    Article  CAS  Google Scholar 

  4. S. P. Arya, M. Mahajan, and P. Jain, Anal. Chim. Acta, 2000, 417, 889.

    Article  Google Scholar 

  5. M. C. Yebra-Biurrum, Talanta, 2000, 52, 367.

    Article  Google Scholar 

  6. H. A. Tajmir-Riahi, J. Inorg. Biochem., 1990, 40, 181.

    Article  CAS  Google Scholar 

  7. H. A. Tajmir-Riahi, J. Inorg. Biochem., 1991, 42, 47.

    Article  CAS  Google Scholar 

  8. Y. Fujita, I. Mori, M. Yoyoda, and K. Taguchi, Bunseki Kagaku, 1993, 42, T1.

    Article  CAS  Google Scholar 

  9. Y. Fujita, I. Mori, and T. Matsuo, Anal. Sci., 1996, 12, 115.

    Article  CAS  Google Scholar 

  10. Y. Nakagawa, I. Mori, T. Matsuo, Y. Shigemura, and Y. Fujita, Bunseki Kagaku, 1999, 48, 935.

    Article  CAS  Google Scholar 

  11. H. Sano, Bull. Chem. Soc. Jpn., 1958, 31, 974.

    Article  CAS  Google Scholar 

  12. Y. Fujita, I. Mori, and T. Matsuo, Anal. Sci., 1998, 14, 1157.

    Article  CAS  Google Scholar 

  13. H. Kohara, Bunseki Kagaku, 1975, 24, 525.

    Article  CAS  Google Scholar 

  14. Y. Fujita, I. Mori, and S. Kitano, Bunseki Kagaku, 1983, 32, E199.

    Article  CAS  Google Scholar 

  15. S. P. Arya and M. Mahajan, Anal. Lett., 1997, 30, 2541.

    Article  CAS  Google Scholar 

  16. M. Tabata and H. Morita, Talanta, 1997, 44, 151.

    Article  CAS  Google Scholar 

  17. P. B. Issopoulos and S. E. Salta, Fresenius J. Anal. Chem., 1997, 357, 317.

    Article  CAS  Google Scholar 

  18. O.-W. Lau, S.-F. Luk, and K.-S. Wong, Analyst [London], 1986, 111, 665.

    Article  CAS  Google Scholar 

  19. T. Pal and T. S. Maity, Anal. Lett., 1985, 18, 1131.

    Article  CAS  Google Scholar 

  20. S. L. C. Ferreira, M. L. S. F. Bandeira, V. A. Lemos, H. C. dos Santos, A. C. S. Costa, and D. S. de Jesus, Fresenius J. Anal. Chem., 1997, 357, 1174.

    Article  CAS  Google Scholar 

  21. J. V. Das, V. K. Gupta, and M. K. Rai, Chem. Anal. [Warsaw], 1998, 43, 85.

    CAS  Google Scholar 

  22. “Commentary of the Japanese Pharmacopoeia, Thirteenth Edition”, 1996, Hirokawa Shoten, Tokyo, C-45.

  23. P. W. Washko, Y. Wang, and M. Levine, J. Biol. Med., 1993, 268, 15531.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Osaka University of Pharmaceutical Sciences, Nasahara, Takatsuki, Osaka, 569-1094, Japan

    Yoshikazu Fujita, Itsuo Mori, Takako Yamaguchi, Mitsuru Hoshino, Yasuyuki Shigemura & Motonobu Shimano

Authors
  1. Yoshikazu Fujita
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  2. Itsuo Mori
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  3. Takako Yamaguchi
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  4. Mitsuru Hoshino
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  5. Yasuyuki Shigemura
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  6. Motonobu Shimano
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Corresponding author

Correspondence to Yoshikazu Fujita.

Additional information

A part of this work was presented at the 58th Symposium on Analytical Chemistry, Fukuoka, 1997.

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Fujita, Y., Mori, I., Yamaguchi, T. et al. Spectrophotometric Determination of Ascorbic Acid with Iron(III) and p-Carboxyphenylfluorone in a Cationic Surfactant Micellar Medium. ANAL. SCI. 17, 853–857 (2001). https://doi.org/10.2116/analsci.17.853

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  • DOI: https://doi.org/10.2116/analsci.17.853

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