Abstract
A new, selective and accurate direct spectrophotometric procedure was developed for the determination of L-ascorbic acid (AA) in pharmaceuticals. Background correction was based on the oxidation of AA by potassium peroxodisulfate in the presence of Cu(II) as a catalyst. The molar absorptivity of the proposed procedure was 1.04 × 104 L/(mol cm) at 262 nm. Beer’s law was obeyed in the concentration range of 0.68–16.00 μg/mL for AA. The detection limit was 0.20 μg/mL, and the relative standard deviation was 0.91% (n = 7) for 8.00 μg/mL AA. Other compounds commonly found in pharmaceutical preparations did not interfere with the detection of AA. The proposed procedure was successfully applied to the determination of AA in pharmaceuticals, and the results obtained agreed with those obtained by iodine titration.
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Fujita, Y., Mori, I., Yamaguchi, T., Hoshino, M., Shigemura, Y., and Shimano, M., Anal. Sci., 2001, vol. 17, no. 7, p. 853.
Janghel, E.K., Gupta, V.K., Rai, M.K., and Rai, J.K., Talanta, 2007, vol. 72, no. 3, p. 1013.
Szpikowska-Sroka, B. and Poledniok, J., J. Anal. Chem., 2011, vol. 66, no. 10, p. 941.
Kato, T., Ohno, O., Nagoshi, T., Ichinose, Y., and Igarashi, S., Anal. Sci., 2005, vol. 21, no. 5, p. 579.
Wu, X., Diao, Y., Sun, C., Yang, J., Wang, Y., and Sun, S., Talanta, 2003, vol. 59, no. 1, p. 95.
Herrero-Martinez, J.M., Simó-Alfonso, E., Deltoro, V.I., Calatayud, A., and Ramis-Ramos, G., Anal. Biochem., 1998, vol. 265, no. 2, p. 275.
Raoof, J.B., Ojani, R., Hosseinzadeh, R., and Ghasemi, V., Anal. Sci., 2003, vol. 19, no. 9, p. 1251.
Iwase, H., Talanta, 2003, vol. 60, no. 5, p. 1011.
Fung, Y.S. and Luk, S.F., Analyst, 1985, vol. 110, no. 2, p. 201.
bin Saari, N., Osman, A., Selamat, J., and Fujita, S., Food Chem., 1999, vol. 66, no. 1, p. 57.
Tsumura, F., Ohsako, Y., Haraguchi, Y., Kumagai, H., Sakurai, H., and Ishii, K., J. Food Sci., 1993, vol. 58, no. 3, p. 619.
Lau, O.W., Luk, S.F., and Wong, K.S., Analyst, 1987, vol. 112, no. 7, p. 1023.
Lau, O.W., Luk, S.F., and Wong, K.S., Analyst, 1986, vol. 111, no. 6, p. 665.
Jung, M.Y., Kim, S.K., and Kim, S.Y., J. Food Sci., 1995, vol. 60, no. 2, p. 360.
Kimura, M., Kobayashi, A., and Boku, K., Bull. Chem. Soc. Jpn., 1982, vol. 55, no. 7, p. 2068.
Davies, M.B., Austin, J., and Partridge, D.A., Vitamin C: Its Chemistry and Biochemistry, London: Royal Society of Chemistry, 1991.
Eitenmiller, R.R. and Landen, W.O., Vitamin Analysis for the Health and Food Sciences, USA: CRC Press, 1999.
Pal, T. and Das, P.K., Anal. Lett., 1988, vol. 21, no. 12, p. 2333.
Hassan, S.S.M., Abd El Fattah, M.M., and Zaki, M.T.M., Fresenius’ Z. Anal. Chem., 1975, vol. 277, no. 5, p. 369.
Backheet, E.Y., Emara, K.M., Askal, H.F., and Saleh, G.A., Analyst, 1991, vol. 116, no. 8. p. 861.
Abdelmageed, O.H., Khashaba, P.Y., Askal, H.F., Saleh, G.A., and Refaat, I.H., Talanta, 1995, vol. 42, no. 4, p. 573.
Arya, S.P., and Mahajan, M., Anal. Lett., 1997, vol. 30, no. 14, p. 2541.
Salinas, F., and Diaz, T.G., Analyst, 1988, vol. 113, no. 11, p. 1657.
Jaselskis, B. and Nelapaty, S.J.J., Anal. Chem., 1972, vol. 44, no. 2, p. 379.
Arya, S.P. and Mahajan, M., Anal. Sci., 1996, vol. 12, no. 6, p. 941.
Ferreira, S.L.C., Bandeira, M.L.S.F., Lemos, V.A., dos Santos, H.C., Costa, A.C.S., and de Jesus, D.S., Fresenius’ J. Anal. Chem., 1997, vol. 357, no. 8, p. 1174.
Fritz, J.S. and Schenk, G.H., Quantitative Analytical Chemistry, New Jersey: Prentice Hall, 1987.
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Salkić, M. Spectrophotometric Determination of L-Ascorbic Acid Based on Its Oxidation by Potassium Peroxodisulfate in the Presence of Cu(II) as Catalyst. J Anal Chem 71, 153–157 (2016). https://doi.org/10.1134/S1061934816020088
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DOI: https://doi.org/10.1134/S1061934816020088