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Extraction of Proteins Based on Phase Separation in Aqueous Triblock Copolymer Surfactant Solutions

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Abstract

Triblock copolymer surfactants, Pluronic L61 (L61) and Pluronic 25R2 (25R2), consisting of poly(ethylene oxide) (PEO) and poly(propylene oxide) (PPO) were utilized for the extraction of proteins. The extraction was based on phase separation into surfactant-depleted and -condensed phases (an aqueous phase and a surfactant-rich phase) upon warming aqueous solutions of L61 and 25R2. Hydrophilic cytochrome c was not extracted into the surfactant-rich phase in either Pluronics. However, hydrophobic cytochrome b5 was extracted with L61, while not with 25R2. The difference in the extractability of cytochrome b5 and cytochrome c is probably due to the structural difference in L61 and 25R2

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References

  1. T. Nakagawa and K. Shinoda, in “Colloidal Surfactants: Some Physicochemical Properties”, ed. K. Shinoda, T. Nakagawa, B. Tamamushi and T. Isemura, p. 97, Academic Press, New York, 1963.

  2. H. Watanabe and H. Tanaka, Talanta, 25, 585 (1978).

    Article  CAS  Google Scholar 

  3. W. L. Hinze and E. Pramauro, Crit. Rev. Anal. Chem., 24, 133 (1993).

    Article  CAS  Google Scholar 

  4. A. Sáchez-Ferrer, R. Bru and F. García-Carmona, Crit. Rev. Biochem. Mol. Biol, 29, 275 (1994).

    Article  Google Scholar 

  5. T. Saitoh, H. Tani, T. Kamidate and H. Watanabe, Trends Anal. Chem., 14, 213 (1995).

    CAS  Google Scholar 

  6. H. Tani, T. Kamidate and H. Watanabe, J. Chromatogr. A, in press (1997).

    Google Scholar 

  7. T. Saitoh, M. Ohba, T. Kamidate and H. Watanabe, Anal. Sci., 10, 379 (1994).

    Article  CAS  Google Scholar 

  8. T. Saitoh, H. Tani, T. Kamidate, T. Kamataki and H. Watanabe, Anal. Sci., 10, 299 (1994).

    Article  CAS  Google Scholar 

  9. H. Tani, T. Saitoh, T. Kamidate, T. Kamataki and H. Watanabe, Biotecknol. Bioeng., in press (1997).

    Google Scholar 

  10. H. Walter, D. E. Brooks and D. Fisher (ed.), “Partitioning in Aqueous Two-Phase Systems - Theory, Methods, Uses, and Applications to Biotechnology”, Academic Press, Orlando, 1985.

    Google Scholar 

  11. P.-A. Albertsson, “Partition of Cell Particles and Macromolecules”, Wiley-Interscience, New York, 1986.

    Google Scholar 

  12. U. Sivars, K. Bergfeldt, L. Piculell and F. Tjerneld, J. Chromatogr. B, 680, 43 (1996).

    Article  CAS  Google Scholar 

  13. I. R. Schmolka, in “Nonionic Surfactants”, ed. M. J. Schick, p. 300, Marcel Dekker, Inc., New York, 1967.

  14. Z. Zhou and B. Chu, J. Colloid Interface Sci., 126, 171 (1988).

    Article  CAS  Google Scholar 

  15. P. N. Hurter, J. M. H. M. Scheutjens and T. A. Hatton, Macromolecules, 26, 5592 (1993).

    Article  CAS  Google Scholar 

  16. P. Linse, Macromolecules, 26, 4437 (1993).

    Article  CAS  Google Scholar 

  17. P. Linse, J. Phys. Chem., 97, 13896 (1993).

    Article  CAS  Google Scholar 

  18. K. Mortensen and J. S. Pedersen, Macromolecules, 26, 805 (1993).

    Article  CAS  Google Scholar 

  19. K. Mortensen, W. Brown and E. Jørgensen, Macromolecules, 27, 5654 (1994).

    Article  CAS  Google Scholar 

  20. F. Gadelle, W. J. Koros and R. S. Schechter, Macromolecules, 28, 4883 (1995).

    Article  CAS  Google Scholar 

  21. A. V. Kabanov, I. R. Nazarova, I. V. Astafieva, E. V. Batrakova, V. Y. Alakhov, A. A. Yaroslavov and V. A. Kabanov, Macromolecules, 28, 2303 (1995).

    Article  CAS  Google Scholar 

  22. K. Zhang and A. Khan, Macromolecules, 28, 3807 (1995).

    Article  CAS  Google Scholar 

  23. K. Mortensen, Macromolecules, 30, 503 (1997).

    Article  CAS  Google Scholar 

  24. M. Svensson, F. Joabsson, P. Linse and F. Tjerneld, J. Chromatogr. A, 761, 91 (1997).

    Article  CAS  Google Scholar 

  25. T. Kamataki, K. Maeda, Y. Yamazoe, T. Nagai and R. Kato, Biochem. Biophys. Res. Commun., 103, 1 (1981).

    Article  CAS  Google Scholar 

  26. A. Nag, G. Mitra and P. C. Ghosh, Anal. Biochem., 237, 224 (1996).

    Article  CAS  Google Scholar 

  27. Product Information Publication, “Pluronic and Tetronic Surfactants”, BASF, 1992.

  28. K. Weckström and M. Zulauf, J. Chem. Soc. Faraday Trans. 1, 81, 2947 (1985).

    Article  Google Scholar 

  29. C. R. Parish, B. J. Classon, J. Tsagaratos, I. D. Walker, L. Kirszbaum and I. F. C. McKenzie, Anal. Biochem., 156, 495 (1986).

    Article  CAS  Google Scholar 

  30. W. J. Horvath and C. W. Hiue, Talanta, 39, 487 (1992).

    Article  CAS  Google Scholar 

  31. B. Fricke, Anal Biochem., 212, 154 (1993).

    Article  CAS  Google Scholar 

  32. A. N. Semenov, J.-F. Joanny and A. R. Khokhlov, Macromolecules, 27, 5654 (1994).

    Article  Google Scholar 

  33. T. Saitoh and W. L. Hinze, Talanta, 42, 119 (1995).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Bioanalytical Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, 060, Japan

    Hirofumi Tani, Akira Matsuda, Tamio Kamidate & Hiroto Watanabe

Authors
  1. Hirofumi Tani
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  2. Akira Matsuda
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  3. Tamio Kamidate
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  4. Hiroto Watanabe
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Tani, H., Matsuda, A., Kamidate, T. et al. Extraction of Proteins Based on Phase Separation in Aqueous Triblock Copolymer Surfactant Solutions. ANAL. SCI. 13, 925–929 (1997). https://doi.org/10.2116/analsci.13.925

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  • DOI: https://doi.org/10.2116/analsci.13.925

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