Skip to main content
SpringerLink
Log in

Assay of NAD+-Isocitrate Dehydrogenase in Extracts of Bread Yeast with a Surface Acoustic Wave Impedance Sensor

  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

The regulatory property of NAD+-isocitrate dehydrogenase (NADMCDH) was studied by means of a new device, a surface acoustic wave (SAW) impedance sensor, which comprises a 61 MHz SAW resonator and a pair of parallel platinum electrodes. The NAD+-ICDH is an allosterically regulated enzyme which shows sigmoidal kinetic for DL-isocitrate. The Hill coefficient was estimated to be 2.93. A linear relationship between the frequency response and the enzyme concentration was obtained, and the experimental detection limit was evaluated to be 45 mlU/ml. A comparison between the SAW impedance sensor and the conductometric method was carried out. The effects of AMP, ADP and ATP on the kinetic property of NAD+-ICDH were investigated; some other factors, such as the temperature and pH value, are also discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. L. A. Sazanov and J. B. Jackson, FEBS Lett., 344, 109 (1994).

    Article  CAS  Google Scholar 

  2. R. C, Jill and L. McAlister-Henn, Biochemistry, 32, 9323 (1993).

    Article  Google Scholar 

  3. Beijing University Biochemistry Teaching and Researching Group, “Guide of biochemistry experiments (in Chinese)”, p. 177, Higher Education Press, Beijing, 1988.

    Google Scholar 

  4. M. S. Nieuwenhuizen and A. J. Nederlof, Anal Chem., 60, 236 (1988).

    Article  CAS  Google Scholar 

  5. A. D. Amico and E. Verona, Seneors Actuators, 17, 55 (1989).

    Article  Google Scholar 

  6. C. G. Fox and J. F. Alder, Analyst [London], 114, 997 (1989).

    Article  CAS  Google Scholar 

  7. D. S. Ballantine and H. Wohltjen, Anal Chem. 61,704A (1989).

    CAS  Google Scholar 

  8. D. S. Ballantine, S. L. Rose, J. W. Grate and H. Wohltjen, Anal. Chem., 58, 3058 (1986).

    Article  CAS  Google Scholar 

  9. A. J, Ricco, S. J. Matin and T. E. Zipperian, Sensors Actuators, 8, 319 (1985).

    Article  CAS  Google Scholar 

  10. J. E. Roederer and G. J. Bastiaans, Anal Chem., 55, 2333 (1983).

    Article  CAS  Google Scholar 

  11. G. S. Calabrese, H. Wohltjen and M. K. Roy, Anal Chem., 59, 833 (1987).

    Article  CAS  Google Scholar 

  12. S. Yao, K. Chen and L. Nie, Anal Chim. Acta, 289, 47 (1994).

    Article  CAS  Google Scholar 

  13. S. Yao, K. Chen, F. Zhu, D. Shen and L. Nie, Anal Chim. Acta, 287, 65 (1994).

    Article  CAS  Google Scholar 

  14. D. Liu, K. Chen, K. Ge, L. Nie and S. Yao, Chinese J. Chem., 13, 232 (1995).

    Google Scholar 

  15. K. Ge, D. Liu, K. Chen, L. Nie and S. Yao, Anal Biochem., 226, 207 (1995).

    Article  CAS  Google Scholar 

  16. K. Ge, D. Liu, K. Chen, L. Nie and S. Yao, Fresenius’ J. Anal Chem., (in press) (1995).

    Google Scholar 

  17. T. Palme, “Understanding Enzyme”, Chap. 9, Ellis Horwood, Chichester, 1981.

    Google Scholar 

  18. J. N. Benjamin, R, M. Michel and M. D. Richard, Biochem. J., 303, 461 (1994).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China

    Ronghui Wang, Qingyun Cai, Lihua Nie & Shouzhuo Yao

Authors
  1. Ronghui Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qingyun Cai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lihua Nie
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shouzhuo Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, R., Cai, Q., Nie, L. et al. Assay of NAD+-Isocitrate Dehydrogenase in Extracts of Bread Yeast with a Surface Acoustic Wave Impedance Sensor. ANAL. SCI. 12, 449–453 (1996). https://doi.org/10.2116/analsci.12.449

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.12.449

Keywords

Search

Navigation