摘要
目的
通过转录组学分析挖掘与新疆梨果中绿原酸生物合成相关的功能基因, 为未来新疆梨的功能性育种提供科学依据。
创新点
明确了在三种新疆梨果发育过程中, 与绿原酸含量变化趋势和绿原酸生物合成相关的差异基因及关键限制性酶。
方法
在鸭梨、库尔勒香梨和圆黄等三种梨果的不同发育阶段采集果实样品, 经液相色谱法分析不同发育期果品的绿原酸含量和熊果苷含量差异, 然后选择花后88、103和163天这三个绿原酸含量变化典型的时间点样品提取RNA, 进行转录组测序, 采取无参转录组学方法挖掘梨果中绿原酸生物合成途径的关键酶基因。
结论
绿原酸含量总体随着梨果的发育呈下降趋势。筛选得到10个与绿原酸生物合成相关的差异基因, 分别为2个4CL基因、1个C4H基因、2个C3’H基因和5个HCT基因, 其中绿原酸生物合成下游途径的C3’H和HCT是关键的限速酶基因。
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Acknowledgments
This work was supported by the Major Scientific and Technological Projects of the Xinjiang Production and Construction Corps (Nos. 2017DB006 and 2020KWZ-012). We thank Prof. Liang LIU from Department of Statistics, the University of Georgia, the United States, for his helps on designing research and revising manuscript.
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Hao WEN performed the experimental research and data analysis, wrote and edited the manuscript. Xi JIANG, Wenqiang WANG, Minyu WU, and Hongjin BAI collected and analyzed the data. Cuiyun WU and Lirong SHEN designed the experimental research, and wrote and revised the manuscript. All authors have read and approved the final manuscript, and therefore, have full access to all the data in the study and take responsibility for the integrity and security of the data.
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Hao WEN, Xi JIANG, Wenqiang WANG, Minyu WU, Hongjin BAI, Cuiyun WU, and Lirong SHEN declare that they have no conflict of interest.
This article does not contain any studies with human or animal subjects performed by any of the authors.
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Comparative transcriptome analysis of candidate genes involved in chlorogenic acid biosynthesis during fruit development in three pear varieties of Xinjiang Uygur Autonomous Region
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Wen, H., Jiang, X., Wang, W. et al. Comparative transcriptome analysis of candidate genes involved in chlorogenic acid biosynthesis during fruit development in three pear varieties of Xinjiang Uygur Autonomous Region. J. Zhejiang Univ. Sci. B 23, 345–351 (2022). https://doi.org/10.1631/jzus.B2100673
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DOI: https://doi.org/10.1631/jzus.B2100673