Abstract
Worldwide there has been a significant increase in the incidence of oropharyngeal squamous cell carcinoma (OPSCC) etiologically attributed to oncogenic human papillomavirus (HPV). Reliable and accurate identification and detection tools are important as the incidence of HPV-related cancer is on the rise. Several HPV detection methods for OPSCC have been developed and each has its own advantages and disadvantages in regard to sensitivity, specificity, and technical difficulty. This review summarizes our current knowledge of molecular methods for detecting HPV in OPSCC, including HPV DNA/RNA polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), p16 immunohistochemistry (IHC), and DNA/RNA in situ hybridization (ISH) assays. This summary may facilitate the selection of a suitable method for detecting HPV infection, and therefore may help in the early diagnosis of HPV-related carcinoma to reduce its mortality, incidence, and morbidity.
概要
在世界范围内,人乳头瘤病毒(HPV)的致癌性 导致口咽鳞状细胞癌(OPSCC)的发病率显著增 加。随着HPV 相关癌症发病率的上升,寻找可 靠且准确的识别和检测HPV 的工具变得非常重 要。目前,虽然在OPSCC 组织中检测HPV 的方 法已有多种,但是在灵敏度、特异性和技术难度 等方面各有优缺点。本文综述了近年来应用于 OPSCC 组织中检测HPV 的方法,包括HPV DNA/RNA 聚合酶链反应(PCR)、环介导等温扩 增(LAMP)、肿瘤蛋白16(p16)免疫组化(IHC) 和DNA/RNA 原位杂交分析(ISH),对选择合适 的HPV检测方法具有指导意义,从而有助于HPV 相关癌症的早期诊断,降低其死亡率和发病率。
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We thank Ms. Syaidatul Akmal SYAIFUDDIN from School of Dental Sciences, Universiti Sains Malaysia for her efforts in editing the figures.
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Fatin Hazwani FAUZI and Nural Izzati HAMZAN analyzed the literature and prepared the first draft of the manuscript. Nurhayu Ab RAHMAN and Siti SURAIYA edited and checked the final version. Suharni MOHAMAD designed, revised, edited, and checked the final version. All authors have read and approved the final manuscript and, therefore, have full access to all the data in the study and take responsibility for the integrity and security of the data.
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Fatin Hazwani FAUZI, Nurul Izzati HAMZAN, Nurhayu Ab RAHMAN, Siti SURAIYA, and Suharni MOHAMAD declare that they have no conflict of interest.
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Project supported by the Universiti Sains Malaysia Research University Grant (1001/PPSG/8012345)
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Fauzi, F.H., Hamzan, N.I., Rahman, N.A. et al. Detection of human papillomavirus in oropharyngeal squamous cell carcinoma. J. Zhejiang Univ. Sci. B 21, 961–976 (2020). https://doi.org/10.1631/jzus.B2000161
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DOI: https://doi.org/10.1631/jzus.B2000161