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Antioxidant activity of alcohol aqueous extracts of Crypthecodinium cohnii and Schizochytrium sp.

隐甲藻和裂殖壶藻醇水提物不同极性萃取物的抗氧化活性研究

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Abstract

Crypthecodinium cohnii (dinoflagellate) and Schizochytrium sp. (thraustochytrid) are the main sources for docosahexaenoic acid (DHA). The present study aimed to evaluate the antioxidant activity of petroleum ether, ethyl acetate, n-butanol, and water fractions of alcohol aqueous extracts of these two microalgae and to provide a theoretical basis for comprehensive utilization. The antioxidant activity was determined by total antioxidant capacity (TAC) determination, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, ferrous ion-chelating ability (FICA) assay, and reducing power (RP) assay. The total phenolic content (TPC) and total flavonoid content (TFC) were also measured by the Folin-Ciocalteu and spectrophotometry methods, respectively. The results indicated that the extracts from these two microalgae possessed good antioxidant capacity. Analysis showed that most antioxidant performance indicators (TAC, DPPH, and RP) were positively correlated with the TPC of the extracts, suggesting that the phenolics might be the major components in C. cohnii and Schizochytrium sp., contributing to their antioxidative function. Therefore, the polar fractions of C. cohnii and Schizochytrium sp. could be further examined and considered for application in health products or cosmetics.

中文概要

目的

评价隐甲藻和裂殖壶藻醇水提物的石油醚萃取 相、乙酸乙酯萃取相、正丁醇萃取相和水相的抗 氧化活性水平,为综合利用两种微藻提供理论依 据。

创新点

隐甲藻和裂殖壶藻是二十二碳六烯酸(DHA)的 重要原料,然而提取DHA 后剩余的藻渣未得到 充分利用。本文首次对两种微藻的极性提取物的 不同极性部位进行抗氧化活性评价,并初步确定 多酚类化合物是其发挥抗氧化作用的主要物质成 分,为综合利用两种微藻提供了理论依据和参考。

方法

用70%乙醇浸提隐甲藻和裂殖壶藻藻粉,所得粗 提物分别使用石油醚、乙酸乙酯、正丁醇和水依 次萃取,得到不同极性组分萃取物。采用总抗氧 化能力、2,2-二苯基-1-三硝基苯肼(DPPH)自由 基清除能力、亚铁离子螯合能力及总还原力等方 法对不同极性组分的萃取物进行抗氧化活性评 价。采用Folin-Ciocalteu 方法测定样品中总多酚 含量,采用分光光度法测定样品中总黄酮含量。

结论

本实验结果显示,隐甲藻和裂殖壶藻醇水提物的 不同极性组分具有较好的抗氧化作用(图2~5)。 相关性分析结果表明萃取物中的多酚类化合物 与其抗氧化水平显著相关(表2 和表3),因此, 我们推测多酚类化合物是两种微藻的主要抗氧 化成分。综上所述,隐甲藻和裂殖壶藻具有综合 开发利用潜力,可深入研究。

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Authors and Affiliations

  1. Institute of Genome Engineered Animal Models for Human Disease, Dalian Medical University, Dalian, 116044, China

    Jin-hui Yu, Jie Sun & Ying-jie Wu

  2. Shandong Center of Crop Germplasm Resources, Jinan, 250100, China

    Jin-hui Yu

  3. School of Life Sciences, Shandong University, Jinan, 250100, China

    Yu Wang

  4. College of Integrative Medicine, Dalian Medical University, Dalian, 116044, China

    Jin-hui Yu, Jie Sun & Ying-jie Wu

  5. Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, 250100, China

    Jin-hui Yu, Fei Bian, Gao Chen, Yan Zhang & Yu-ping Bi

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  1. Jin-hui Yu
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  2. Yu Wang
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Correspondence to Yu-ping Bi or Ying-jie Wu.

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Project supported by the Shandong Academy of Agriculture Sciences (No. 2015YQN32), the Ministry of Science and Technology of China (No. 2014DFA32120), and the National Natural Science Foundation of China (No. 81471000)

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Yu, Jh., Wang, Y., Sun, J. et al. Antioxidant activity of alcohol aqueous extracts of Crypthecodinium cohnii and Schizochytrium sp.. J. Zhejiang Univ. Sci. B 18, 797–806 (2017). https://doi.org/10.1631/jzus.B1600367

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