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Toxicity comparison of different active fractions extracted from radix Sophorae tonkinensis in zebrafish

使用斑马鱼模型比较山豆根不同有效部位的毒性

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Abstract

Radix Sophorae tonkinensis (RST) is a widely used herb in Traditional Chinese Medicine (TCM) for treating infectious and inflammatory diseases. However, the toxicity data for RST are limited. The aim of this work is to assess and compare the toxicity of the whole RST extract and its five active fractions using the zebrafish model. Five active fractions of RST were prepared using five different types of solvents, which included dealkalized water, ethanol, n-butyl ethanol, dichloromethane, and diethyl ether. The chemical profiles of the active fractions were determined by high-performance liquid chromatography (HPLC), and the toxicity observed in the zebrafish model was confirmed using mouse models. In the zebrafish model, cardiovascular toxicity was observed for the fraction extracted using diethyl ether, and hepatotoxicity was observed for the whole RST extract and the fractions extracted using water and ethanol, whereas both cardiovascular and hepatic toxicities were observed for the fractions extracted using n-butyl ethanol and dichloromethane. The hepatotoxicity of the fractions extracted using n-butyl ethanol and dichloromethane was also observed in mice. Our findings provide the toxicity data for RST and its five active fractions through modeling in a zebrafish, and indicate that the different fractions may each have a different toxicity, which is helpful for the optimal use of RST in clinical practice.

中文概要

目的

利用斑马鱼模型评价和比较山豆根不同提取方法 提取的有效部位的体内毒性。

创新点

首次在斑马鱼模型中证明山豆根提取方法不同, 有效部位的毒性有明显差异。研究结果有助于指 导山豆根的新药开发与临床应用。

方法

用五种不同的溶剂(去碱水、乙醇、正丁基乙醇、 二氯甲烷和乙醚)提取山豆根,然后通过高效液 相色谱法(HPLC)检测有效部位,将AB 品系斑 马鱼分为对照组(养鱼水处理)和实验组(山豆 根提取物)。实验组根据采用的提取溶剂不同, 分为以下六组:去碱水提取组、乙醇沉提取组、 正丁基乙醇提取组、二氯甲烷提取组和乙醚提取 组以及山豆根总组分组(对照),观察各种山豆 根提取物对斑马鱼的急性毒性与毒性靶器官。

结论

山豆根乙醚提取组诱发斑马鱼心血管毒性(图1); 山豆根去碱水提取组、乙醇沉提取组以及山豆根 总组分组诱发斑马鱼肝脏毒性(图3 和图4);而 山豆根正丁基乙醇提取组和二氯甲烷提取组诱 发斑马鱼心血管毒性(图1 和图2)和肝脏毒性 (图3 和图4)

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Authors and Affiliations

  1. Center for Drug Safety Evaluation and Research, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China

    Hong-cui Liu, Jiang-hua Chen & Zhong-ping Deng

  2. Hunter Biotechnology, Inc., Hangzhou, 311231, China

    Hong-cui Liu, Xiao-yu Zhu, Sheng-ya Guo & Chun-qi Li

Authors
  1. Hong-cui Liu
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  2. Xiao-yu Zhu
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  3. Jiang-hua Chen
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  4. Sheng-ya Guo
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  6. Zhong-ping Deng
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Correspondence to Chun-qi Li or Zhong-ping Deng.

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Project supported by the National Science and Technology Major Project of China (Nos. 2012ZX09505001-002 and 2015ZX09501004-002-002) and the Zhejiang Provincial Science and Technology Planning Project of China (No. 2014C03009)

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Liu, Hc., Zhu, Xy., Chen, Jh. et al. Toxicity comparison of different active fractions extracted from radix Sophorae tonkinensis in zebrafish. J. Zhejiang Univ. Sci. B 18, 757–769 (2017). https://doi.org/10.1631/jzus.B1600158

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  • DOI: https://doi.org/10.1631/jzus.B1600158

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