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An Arabidopsis mutant atcsr-2 exhibits high cadmium stress sensitivity involved in the restriction of H2S emission

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Abstract

The gene AtCSR encodes peptidyl-prolyl cis/trans isomerases (PPIases) that accelerate energetically unfavorable cis/trans isomerization of the peptide bond preceding proline production. In our studies, we found that AtCSR was associated with cadmium (Cd)-sensitive response in Arabidopsis. Our results show that AtCSR expression was triggered by Cd-stress in wild type Arabidopsis. The expression of some genes responsible for Cd2+ transportation into vacuoles was induced, and the expression of the iron-regulated transporter 1 (IRT1) related to Cd2+ absorption from the environment was not induced in wild type with Cd2+ treatment. The expression of Cd-transportation related genes was not in response to Cd-stress, whereas IRT expression increased dramatically in atcsr-2 with Cd2+ treatment. The expression of glutathione 1 (GSH1) was consistent with GSH being much lower in atcsr-2 in comparison with the wild type with Cd2+ treatment. Additionally, malondialdehyde (MDA), hydrogen peroxide, and Cd2+ contents, and activities of some antioxidative enzymes, differed between the wild type and atcsr-2. Hydrogen sulfide (H2S) has been confirmed as the third gas-transmitter over recent years. The findings revealed that the expression pattern of H2S-releasing related genes and that of Cd-induced chelation and transportation genes matched well in the wild type and atcsr-2, and H2S could regulate the expression of the Cd-induced genes and alleviate Cd-triggered toxicity. Finally, one possible suggestion was given: down-regulation of atcsr-2, depending on H2S gas-transmitter not only weakened Cd2+ chelation, but also reduced Cd2+ transportation into vacuoles, as well as enhancing the Cd2+ assimilation, thus rendering atcsr-2 mutant sensitive to Cd-stress.

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Authors and Affiliations

  1. School of Life Science, Shanxi University, Taiyuan, 030006, China

    Ya-wei Li, Ze-hua Gong, Yao Mu, Yi-xian Zhang, Zeng-jie Qiao, Li-ping Zhang, Zhu-ping Jin & Yan-xi Pei

  2. School of Chemical Engineering and Environment, North University of China, Taiyuan, 030051, China

    Zhu-ping Jin

  3. School of Environmental Science and Resources, Shanxi University, Taiyuan, 030006, China

    Hua Li

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  1. Ya-wei Li
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  2. Ze-hua Gong
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  3. Yao Mu
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Correspondence to Yan-xi Pei.

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Project supported by the Research Fund for the Doctoral Program of Higher Education of China (No. 20091401110004), the Science and Technology Special Project of Shanxi Province, China (2012, to Qiang ZHANG), and the Shanxi Scholarship Council of China (No. 2011-007)

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Li, Yw., Gong, Zh., Mu, Y. et al. An Arabidopsis mutant atcsr-2 exhibits high cadmium stress sensitivity involved in the restriction of H2S emission. J. Zhejiang Univ. Sci. B 13, 1006–1014 (2012). https://doi.org/10.1631/jzus.B1200089

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