Skip to main content
SpringerLink
Log in

Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease

  • Published:
Journal of Zhejiang University SCIENCE B Aims and scope Submit manuscript

Abstract

Objective

To investigate the effects of mycotoxin moniliformin (MON) on the metabolism of aggrecan and type II collagen in human chondrocytes in vitro and the relationship between MON and Kashin-Beck disease (KBD).

Methods

Human chondrocytes were isolated and cultured on bone matrix gelatin to form an artificial cartilage model in vitro with or without MON toxin. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of aggrecan and type II collagen in the cartilage was determined using immunocytochemical staining.

Results

MON toxin inhibited chondrocyte viability in dose-dependent and time-dependent manners. MON reduced aggrecan and type II collagen syntheses in the tissue-engineered cartilage. MON also increased the expression of matrix metalloproteinase-1 (MMP-1), MMP-13, BC4 epitopes, and CD44 in cartilages. However, the expression of 3B3(−) epitopes in cartilages was inhibited by MON. Selenium partially alleviated the damage of aggrecan induced by MON toxin.

Conclusion

MON toxin promoted the catabolism of aggrecan and type II collagen in human chondrocytes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aigner, T., Stöve, J., 2003. Collagens—major component of the physiological cartilage matrix, major target of cartilage degeneration, major tool in cartilage repair. Adv. Drug Del. Rev., 55(12):1569–1593. [doi:10.1016/j.addr.2003.08.009]

    Article  CAS  Google Scholar 

  • Cao, J.L., Mo, X.Y., Wang, C.Y., 1994. Effects of selenium and zinc on the growth metabolism of cultured chondrocytes. Chin. J. Endemiol., 14(10):628–630 (in Chinese).

    Google Scholar 

  • Cao, J.L., Zheng, B., Zhang, S.Y., Mo, D.X., 1995. The experimental study of moniliformin effects on the chondrocytes. Endemic. Dis. Bull., 10(4):5–7 (in Chinese).

    Google Scholar 

  • Cao, J.L., Zhang, A., Yang, B., Zhang, Z.T., Fu, Q., Hughes, C.E., Caterson, B., 2007. The effect of fungal moniliformin toxin and selenium supplementation on cartilage metabolism in vitro. Osteoarthritis and Cartilage, 15(Suppl. 3):C108. [doi:10.1016/S1063-4584(07)61815-9]

    Article  Google Scholar 

  • Cao, J.L., Li, S., Shi, Z., Yue, Y., Sun, J., Chen, J., Fu, Q., Hughes, C.E., Caterson, B., 2008. Articular cartilage metabolism in patients with Kashin-Beck disease: an endemic osteoarthropathy in China. Osteoarthritis and Cartilage, 16(6):680–688. [doi:10.1016/j.joca.2007.09.002]

    Article  CAS  PubMed  Google Scholar 

  • Caterson, B., Mahmoodian, F., Sorrel, J.M., Hardingham, T.E., Bayliss, M.T., Carney, S.L., Ratcliffe, A., Muir, H., 1990. Modulation of native chondroitin sulfate structure in tissue development and in disease. Cell Sci., 97(3):411–417.

    CAS  Google Scholar 

  • Caterson, B., Carl, R.F., Clare, E.H., Chris, B.L., 2000. Mechanisms involved in cartilage proteoglycan catabolism. Matr. Biol., 19(4):333–344. [doi:10.1016/S0945-053X(00)00078-0]

    Article  CAS  Google Scholar 

  • Cetin, Y., Bullerman, L.B., 2005. Cytotoxicity of fusarium mycotoxins to mammalian cell cultures as determined by the MTT bioassay. Food Chem. Toxicol., 43(5):755–764. [doi:10.1016/j.fct.2005.01.016]

    Article  CAS  PubMed  Google Scholar 

  • Chan, P., Wu, C., 2006. A peptide fragment of type II collagen induces the OA phenotype. Matr. Biol., 25(Supp. 1):S44. [doi:10.1016/j.matbio.2006.08.123]

    Article  Google Scholar 

  • Chen, J.H., Chu, Y.L., Cao, J.L., Yang, Z.T., Guo, X., Wang, Z.L., 2006. T-2 toxin induces apoptosis, and selenium partly blocks, T-2 toxin induced apoptosis in chondrocytes through modulation of the Bax/Bcl-2 ratio. Food Chem. Toxicol., 44(4):567–573. [doi:10.1016/j.fct.2005.09.004]

    Article  CAS  PubMed  Google Scholar 

  • Gelse, K., Poschl, E., Aigner, T., 2003. Collagens—structure, function, and biosynthesis. Adv. Drug Del. Rev., 55(12): 1531–1546. [doi:10.1016/j.addr.2003.08.002]

    Article  CAS  Google Scholar 

  • Hayes, A.J., Hughes, C.E., Caterson, B., 2008. Antibodies and immunohistochemistry in extracellular matrix research. Methods, 45(1):10–21. [doi:10.1016/j.ymeth.2008.01.011]

    Article  CAS  PubMed  Google Scholar 

  • Khan, I.M., Gilbert, S.J., Caterson, B., Sandell, L.J., Archer, C.W., 2008. Oxidative stress induces expression of osteoarthritis markers procollagen IIA and 3B3(−) in adult bovine articular cartilage. Osteoarthritis and Cartilage, 16(6):698–707. [doi:10.1016/j.joca.2007.10.004]

    Article  CAS  PubMed  Google Scholar 

  • Knudson, C.B., Knudson, W., 2001. Cartilage proteoglycans. Sem. Cell Dev. Biol., 12(2):69–78. [doi:10.1006/scdb.2000.0243]

    Article  CAS  Google Scholar 

  • Li, S.J., Yang, L.S., Li, Y.H., Wang, W.L., Xirao, R.D., 2006. Relationship between the content of selenium in grains and the Kaschin-Beck disease in Tibet China. Chin. J. Endemiol., 25(6):673–674 (in Chinese).

    CAS  Google Scholar 

  • Li, S.Y., Cao, J.L., Shi, Z.L., Chen, J.H., Zhang, Z.T., Hughes, C.E., Caterson, B., 2008. Promotion of the articular cartilage proteoglycan degradation by T-2 toxin and selenium protective effect. J. Zhejiang Univ.-Sci. B, 9(1):22–33. [doi:10.1631/jzus.B071322]

    Article  CAS  PubMed  Google Scholar 

  • Li, X.D., Li, J., Balian, G., Laurencin, C.T., Anderson, D.G., 2006. Demineralized bone matrix gelatinas scaffold for osteochondral tissue engineering. Biomaterials, 27(11):2426–2433. [doi:10.1016/j.biomaterials.2005.11.040]

    Article  CAS  PubMed  Google Scholar 

  • Lin, P.M., Chen, C.T.C., Torzilli, P.A., 2004. Increased stromelysin-1 (MMP-3), proteoglycan degradation (3B3- and 7D4) and collagen damage in cyclically load-injured articular cartilage. Osteoarthritis and Cartilage, 12(6):485–496. [doi:10.1016/j.joca.2004.02.012]

    Article  PubMed  Google Scholar 

  • Little, C.B., Hughes, C.E., Curtis, C.L., Janusz, M.J., Bohne, R., Wang-Weigand, S., Taiwo, Y.O., Mitchell, P.G., Otterness, I.G., Flannery, G.R., Caterson, B., 2002. Matrix metalloproteinases are involved in C-terminal and interglobular domain processing of cartilage aggrecan in late stage cartilage degradation. Matr. Biol., 21(3):271–288. [doi:10.1016/S0945-053X(02)00004-5]

    Article  CAS  Google Scholar 

  • Mario Geysen, H., 1985. Antigen-antibody interactions at the molecular level: adventures in peptide synthesis. J. Immunol. Today, 6(12):364–369. [doi:10.1016/0167-5699(85)90096-9]

    Article  Google Scholar 

  • Mo, D.X., Ding, D.X., Wang, Z.L., Zhang, J., Bai, C., 1997. Twenty-year research on selenium related to Kashin-Beck Disease. Xi’an Med. Univ., 9(1):79–89 (in Chinese).

    CAS  Google Scholar 

  • Moreno, R.R., 2009. Iodine, Selenium Deficiency and Kashin-Beck Disease. Comprehensive Handbook of Iodine. Nutritional, Biochemical, Pathological and Therapeutic Aspects, Brussels, Belgium, p.685–700.

  • Nathanson, M.A., Robert Hilfer, S., Searls, R.L., 1978. Formation of cartilage by non-chondrogenic cell types. Dev. Biol., 64(1):99–117. [doi:10.1016/0012-1606(78)90063-5]

    Article  CAS  PubMed  Google Scholar 

  • National KBD Surveillance Group, 2001. The monitoring report of KBD prevalence rate of the whole country in 2001. Chin. J. Endemiol., 20(5):350–353 (in Chinese).

    Google Scholar 

  • Ra, H.J., Parks, W.C., 2007. Control of matrix metalloproteinase catalytic activity. Matr. Biol., 26(8):587–596. [doi:10.1016/j.matbio.2007.07.001]

    Article  CAS  Google Scholar 

  • Roughley, P., Hoemann, C., Rosiers, E.D., Mwale, F., Antoniou, J., Alini, M., 2006. The potential of chitosan-based gels containing intervertebral disc cells for nucleus pulposus supplementation. Biomaterials, 27(3):388–396. [doi:10.1016/j.biomaterials.2005.06.037]

    Article  CAS  PubMed  Google Scholar 

  • Shi, Z.L., Cao, J.L., Chen, J.H., Li, S.Y., Zhang, Z.T., Yang, B., Peng, S.Q., 2009. Butenolide induced cytotoxicity by disturbing the prooxidant-antioxidant balance, and antioxidants partly quenchin human chondrocytes. Toxicol. in Vitro, 23(1):99–104. [doi:10.1016/j.tiv.2008.11.002]

    Article  CAS  PubMed  Google Scholar 

  • Slater, R.R., Michael, T., Bayliss, M.T., Lachiewicz, P.F., Visco, D.M., Caterson, B., 1995. Monoclonal antibodies that detect biochemical markers of arthritis in humans. Arthritis Rheum., 38(5):655–659. [doi:10.1002/art.1780380513]

    Article  PubMed  Google Scholar 

  • Stadtman, L.C., 1980. Biological functions of selenium. Trends Biochem. Sci., 5(8):203–206. [doi:10.1016/S0968-0004(80)80008-9]

    Article  CAS  Google Scholar 

  • Stanton, H., Fosang, A.J., 2002. Matrix metalloproteinases are active following guanidine hydrochloride extraction of cartilage: generation of DIPEN neoepitope during dialysis. Int. Soc. Matr. Biol., 21(5):425–428. [doi:10.1016/S0945-053X(02)00035-5]

    Article  CAS  Google Scholar 

  • Sumer, E.U., Sondergaard, B.C., Rousseau, J.C., Delmas, P.D., Fosang, A.J., Karsdal, M.A., Christiansen, C., Qvist, P., 2007. MMP and non-MMP-mediated release of aggrecan and its fragments from articular cartilage: a comparative study of three different aggrecan and glycosaminoglycan assays. Osteoarthritis and Cartilage, 15(5):594–595. [doi:10.1016/j.joca.2007.02.011]

    Article  Google Scholar 

  • Tadashi, Y., Elena, T., Kunitaka, O., Peter, J.R., William, W., Aisha, M., Mirela, I., Isabelle, P., Robin, P.A., 2006. Peptides of type II collagen can induce the cleavage of type II collagen and aggrecan in articular cartilage. Int. Soc. Matr. Biol., 25:419–429.

    Google Scholar 

  • Tan, J.A., Zhu, W.Y., Wang, W.Y., Li, R.B., Hou, S.F., Wang, D.H., Yang, L.S., 2002. Selenium in soil and endemic diseases in China. Sci. Total Environ., 284(1–3):227–235. [doi:10.1016/S0048-9697(01)00889-0]

    Article  CAS  PubMed  Google Scholar 

  • Warren, K., Geraldine, C., Cheryl, B.K., 2002. CD44-mediated uptake and degradation of hyaluronan. Int. Soc. Matr. Biol., 21:15–23.

    Google Scholar 

  • Xiong, Y.M., Mo, D.X., Li, S.C., Guo, X., Zhang, S.Y., Wang, Z.L., Bi, H.Y., Xu, J.R., 1998. The experimental study of moniliformin and selenium effects on articular cartilage of Chinese experimental mini pig. Endemic Dis. Bull., 3(2):1–3 (in Chinese).

    Google Scholar 

  • Yang, J.B., 2002. Mycotoxins and human diseases. Chin. J. Endemiol., l21(4):314–317 (in Chinese).

    Google Scholar 

  • Zhao, X.J., 2002. Research progress moniliformin. Progress in Veterinary Medicine, 23(4):19–22.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Endemic Diseases, School of Medicine, Xi’an Jiaotong University, Xi’an, 710061, China

    An Zhang, Jun-ling Cao, Bo Yang, Jing-hong Chen, Zeng-tie Zhang & Qiang Fu

  2. Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Xi’an Jiaotong University, Xi’an, 710061, China

    An Zhang, Jun-ling Cao, Bo Yang, Jing-hong Chen, Zeng-tie Zhang & Qiang Fu

  3. Key Laboratory of Microelement and Endemic Disease of Ministry of Health, Xi’an Jiaotong University, Xi’an, 710061, China

    An Zhang, Jun-ling Cao, Bo Yang, Jing-hong Chen, Zeng-tie Zhang & Qiang Fu

  4. Laboratory of Connective Tissue Biology, School of Biosciences, Cardiff University, Cardiff, CF10 3US, UK

    Si-yuan Li, Clare E. Hugnes & Bruce Caterson

Authors
  1. An Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jun-ling Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bo Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jing-hong Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zeng-tie Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Si-yuan Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Qiang Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Clare E. Hugnes
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Bruce Caterson
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Jun-ling Cao or Bruce Caterson.

Additional information

Project supported by the National Natural Science Foundation of China (Nos. 30872187, 30471499, and 30170831), the Ministry of Education of China (No. Key 03152), and the Science Foundation of Shaanxi Province of China (No. 2004KW-20)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, A., Cao, Jl., Yang, B. et al. Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease. J. Zhejiang Univ. Sci. B 11, 200–208 (2010). https://doi.org/10.1631/jzus.B0900074

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1631/jzus.B0900074

Key words

CLC number

Search

Navigation